More than 80 species have been collected and 18 of them including six new species and 12 new geographical records have been worked out in china. they are : comesoma, comesomatidae ; pomponema, cyatholaimidae ; marylynnia, cyatholaimidae ; pterygonema, ceramoneniatidae ; metachromadora, desmodoridae ; desmoscolex, desmoscolecidae aponema, microlaimidae ; gammarinema, monhysteridae ; metadesmolaimus, xyalidae ; rhynchonema, xyalidae ; xyala, xyalidae ; viscosia, oncholaimidae we found divergence in the species composition in different habiting area ( mud flat and sand flat ). the investigation put emphasis on some area, try to know the distribution of marine nematodes for the later research of the position of marine nematodes in the benthic eecological system
這十一個屬包括:
聯體線蟲科( comesomatidae )的
聯體線蟲屬( comesoma ) ;杯咽線蟲科( cyatholaimidae )的華麗線蟲屬( pomponema ) ;杯咽線蟲科( cyatholaimidae )的瑪里林恩線蟲屬( marylynnia ) ;覆瓦線蟲科( ceramonematidae )的翼紋
體線蟲屬( pterygonema ) ;鏈環線蟲科( desmodoridae )的后菱光線蟲屬( metachromadora ) ;微咽線蟲科( microlaimidae )的厚甲線蟲( aponema ) ;單宮線蟲科( monhysteridae )的嗜鯨線蟲屬( gammarinema ) ;希阿利線蟲科( xyalidae )的后棘線蟲屬( metadesmolaimus ) ;希阿利線蟲科( xyalidae )的吻腔線蟲屬( rhynchonema ) ;希阿利線蟲科( xyalidae )的雙翼線蟲屬( xyala ) ;瘤線蟲科( oncholaimidae )的顯齒線蟲屬( viscosia )此外我們報道了六個新種,並對一些已經報道過的蟲種進行重新的描述和討論。
It was established that grb - ast3 concatemer are more easily expressed in the way of fusion expession
初步認為grb - ast _ 3串
聯體基因適宜採用融合表達載
體表達。
Is encoded by grb ~ ast7. the expression analysis of the concatemer was proceeded in the e. coli pet expression system
本文主要工作就是構建grb - ast _ 7串
聯體,將構建后的串
聯體導入大腸桿菌進行表達分析。
Neuropeptide ast2 is encoded by grb - ast3. pcr methods were tried to construct grb - ast3 concatemer. the expression analysis of the concatemer was proceeded in the e. coli pet expression system
本論文採用改進的pcr 「自身模板-引物」法進行構建方法構建grb - ast _ 3串
聯體,並將拼接好的串
聯體轉入大腸桿菌進行表達分析。
Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase. one concatemer of 360bp, 9 copies of grb - ast3, were obtained from the first pair of primer and three ones of 515bp, 750bp, 792bp, from the second one
從第一對引物的拼接產物中克隆到一個360bp含有9個拷貝數的grb - ast _ 3的串
聯體,從第二對引物的拼接產物中克隆到三個分別為515 、 750 、 792bp的grb - ast _ 3的串
聯體。
In this method, high fidelity dna polymerase ( pyrobesf * dna polymerase ) was used to ensure efficient and accurate amplification. after cloning and sequencing of the pcr products, one concatemer contains seventeen copies of grb ~ ast7 was attained. the grb - ast ? concatemer was cloned into pet22b expression vector
先用t4dnapolymerase構建成模板,再通過高保真dna聚合酶( pyrobesttmdnapolymerase ) pcr進行擴增,從中得到一個含有17個拷貝的grb - ast _ 7的串
聯體。
Objective to report a case of conjoined twins with peripheral angiography together with cardioangiographic technique
摘要目的報導1例胸腹
聯體兒心臟與外周血管造影檢查,介紹心血管造影技術。
Based on summing up construction controlling technology of cable - stayed bridges of home and abroad, taking qingdao danshan cable - stayed bridge as project background, this paper has a particular study to the construction controlling for the twin - united body one pylon cable - stayed bridge
本文在總結國內外斜拉橋施工控制技術的基礎上,以青島丹山斜拉橋為工程背景,就雙聯體獨塔斜拉橋施工控制問題,有針對性的展開研究。