肽基位或 的英文怎麼說
中文拼音 [tàijīwèihuò]
肽基位或
英文
peptidyl site-
The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification
本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq
然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。Patients with above - median mpo levels in combination with above - median plasma amino - terminal pro - brain natriuretic peptide ( nt - probnp ) or below - median left ventricular ejection fraction ( lvef ) had significantly greater mortality compared with other patients
在mpo中位數以上水平伴血漿氨基末端前腦利鈉肽中位數水平以上病人或左心室射血分數在中位數以下病人相對于其他病人有更高的死亡率。These phage display peptides may be the tnfa mimotopes. part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding - peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides. we used c7c tnfa mimotope phage clone lcs - 7 ( c - rrpaqsg - c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa
3 、以特異性噬菌體克隆為靶篩選肽庫的研究? ?一種肽庫篩選新方法的建立：為了深入研究細胞因於與配基（受體或抗體）相互作用的關鍵殘基及其空間構象，在上述研究基礎上，我們以tnfa表位模擬肽克隆為靶篩選噬菌體十二肽庫，找尋與之結合的tnfa結合肽噬菌體克隆；並以此拓寬了噬菌體肽庫的應用范圍。Deuterium for hydrogen exchange at amide sites in proteins is a well - established means of probing the stability of certain proteins and the effects of interactions with ligands and other proteins
肽鍵位置上以氘代氫是檢測某些蛋白質穩定性及其與配基或其他蛋白質相互作用的成熟方法。分享友人