胎牛血清 的英文怎麼說

中文拼音 [tāiniúxiěqīng]
胎牛血清 英文
dialyzed fbs
  • 胎 : 名詞1 （幼體） foetus; embryo 2 （懷孕或生育的次數） birth 3 （衣服、被褥等的面子和裡子之間的襯物...
  • 牛 : 名詞1. （哺乳動物） cattle; ox 2. （姓氏） a surname
  • 血 : 血名詞（血液 多用於口語） blood:吐血 spit （up） blood; 血的教訓 a lesson paid for [written] in b...
  • 清 : Ⅰ形容詞1 （純凈） unmixed; clear 2 （寂靜） quiet 3 （清楚） distinct; clarified 4 （一點不留） w...
  • 血清 : [免疫學] serum; blood serum血清病 serum sickness
  1. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  2. Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus, two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196. after 6 days cryopreservation, one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other, cryopreserved for 13 days, was 52

    以細胞培養液m199 （含20 %的小牛血清）為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，採取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞於一1 %的液氮中。第一組冷凍保存6天後解凍，成活率為72 % ，第二組冷凍保存13天後解凍，成活率為520 / 0 。
  3. The numhers of day - 4 ebs per milliliter culture media are 44. 83 1. 22 ( n = 6 ) and 43. 17 1. 05 ( n = 6 ) respectively when cultured by hyclone fbs and homemade green season fbs

    在含進口hyclone胎牛血清或國產四季青胎牛血清的培養液中培養， es d3細胞形成的4debs數量分另為44
  4. But in experiment ii, the obtained result of measurement and examination is not better than control group 。 conclusion : mixed animo acids can be safely applyed to vessel cryopreservation instead of calf serum 。 the effect of the groups of the rapid freezing method is not better than the groups of the rate - controled slow freezing

    而實驗ii的血管其內皮結構不清晰，內皮細胞超微結構變化較大。結論：復方氨基酸可代替胎牛血清用於血管的深低溫保存.速凍降溫方式對血管深低溫保存效果不及慢凍降溫方式。
  5. Method : 120 rabbit vessels were randomly divided into three groups : the vessel of control group in cryopreservative medium containing calf serum were subjeted to rate - controled slow freezing ; those of experiment group i in cryopreservative medium containing mixed animo acids were frozen by the same method as in control group ; and those of experiment group ii in the same cryopreserative medium as in control group were subjected to rapid freezing

    材料和方法：取中國純種家兔股動脈120段，隨機分為3組：對照組用含胎牛血清的保護液處理，以慢凍方式降溫；實驗組1用含復方氨基酸的保護液處理，以慢凍方式降溫；實驗組ii用含胎牛血清的保護液處理，用速凍方式降溫。
  6. Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell

    經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。
  7. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  8. Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum

    含胎牛血清膠原酶消化法培養兔成骨細胞
  9. Methods rabbit conjunctival epithelial tissues were explanted in serum - free system the morphology, gowth, proliferation and differentiation of outgrow cells were compared with those in serum - containing medium

    方法分別用含胎牛血清培養液和不含血清培養液進行組織塊培養，觀察兩種培養方法在細胞形態、細胞生長情況和增殖及細胞分化方面的差異。
  10. The culture results of the porcine ear skin fibroblasts was good by using dmem / f12 containing 20 % fetal bovine serum

    在培養條件方面選取dmem f12培養液+ 20胎牛血清培養豬耳皮膚成纖維細胞，效果較好。
  11. Pbmc were cultured in complete medium and supplemented with 1000u / ml rhgm - csf and 500u / ml rhil - 4, modc were collected on day 9. tumor cells were cultued with 10 % complete medium

    腫瘤細胞的培養將mgc803 、 ls174t 、 ec109細胞分別培養于含10胎牛血清青霉素100n分ml ，鏈黴素100n分ml的rpmi
  12. Results isolation, culture and purification of the mmscs typically, mmscs were isolated from bone marrow by their adherence to plastic and grew as fibroblastic cells that developed into visible symmetric colonies

    顯色15 30分鐘。結果1 mmscs分離、培養及純化用含10胎牛血清的dmem ? lg培養基在玻璃培養瓶中培養骨髓單個核細胞。
  13. Methods isolation, culture and purification of mouse mscs mmscs were isolated from the femurs of adult mice four weeks old and propagated in vitro. mmscs were originally cultured in dmem - lg with 10 % fbs, 100u / ml penicillion, 100mg / ml streptomycin

    在細胞沉澱中加人含10胎牛血清的dmem lg培養基，計數細胞數，調整細胞密度，按3x10vinl接種於25cm 『的玻璃培養瓶中。
  14. We can see a lot of new pictures of induced neuron - like cells, include neuron - like cells and glial - like cells, with the apparent nerve cells. material and methods rat bone marrow cell were collected, after sacrifice of a 3 months old wistar rat, from femurs and tibias by flushing the shaft with culture media ( dmem - 10 % fetal bovine serum ) using a syringe of 5ml

    無菌條件下取股骨和胚骨的骨髓b人snd含10胎牛血清的dmem培養液1000rpm離心10分鐘，吹打b人兩個培養瓶，置人37 j coh飽和濕度條件下培養，隔日更換培養液，當細胞達到70融合時，傳代。
  15. The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively

    主要內容和結果如下： （一）黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，採用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
  16. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  17. The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates

    加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。
  18. The enhancing factors of eb formation were studied. fifty thousand es - d3 cells per milliliter were incubated in dmem - high glucose supplemented with 20 % fbs and 2mm glutamine. the influencing factors which included hyclone fbs and homemade green season fbs, feeder cell layer, culture containers and 3 - mercaptoethanol ( - me ) were investigated

    以5x10 』加的eso3細胞種入含20胎牛血清和zinm谷氨酚胺的高糖dmem中，觀察進口胎牛血清和國產胎牛血清、飼養層、培養器皿以及卜硫基乙醇對es士3細胞形成的4debs數量的影響。
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