Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞
胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純
化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉
化大腸桿菌jm109感受態細胞,轉
化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1 - dimention and 2 - dimention nuclear magnetic resonance ( nmr ) methods. this work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process. also, it provided a new way to study the process of embryo developing
用一維及二維液體高分辨核磁共振( nuclearmagneticresonance , nmr )方法綜合分析了雞
胚羊水和尿囊液的成分,對其中的大多數譜峰進行了歸屬,發現了一些未經報道的小分子代謝物,為研究
胚胎發育過程中各個
胚囊中體液的交換和小分子的代謝以及進一步對發育過程中代謝物含量變
化的定量研究打下了基礎,並為研究
胚胎發育的過程提供了新的思路。
Impressive metabolic alternations have been undergone during embryogenesis.
在
胚胎發生期間經歷了深刻的代謝變
化。
It lasted for more than four months from initiation of the ovulate strobilus to formation of anatropous ovules
從雌球果原基發生到
胚珠分
化歷時4個多月。
The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells
研究初步表明:以細胞培養液m199 (含2既的小牛血清,常規量雙抗)為凍存稀釋液對泥鰍
胚胎細胞冷凍保存宜採取先慢后快的方式(例如,從0一一6 ,凍存速度為一0 . 05 / min ,再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍
胚胎細胞的成活率影響不明顯;囊
胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養,解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁,但只發現兩瓶培養細胞有明顯增殖現象產生許多未分
化的小細胞。
We specialize in manufacturing top grade bone china, intensified porcelain and white jade - like porcelain from the ambryonic design, coloured drawing or pattern and firing to design paper printing and packing
專業生產高檔骨瓷強
化瓷白玉瓷。從白
胚製作彩繪燒成至花紙印刷及包裝等一條生產。
The hatching enzyme from brine shrimp, artemia shrimp, is a pivotal protease which help the encysted embryo escape from its hatching membrane when hatching
鹵蟲孵
化酶( he )是由鹵蟲早期
胚胎特異性分泌的、在孵
化過程中起關鍵作用的一種蛋白酶。
A partially purified concentrate of the solid endosperm is active in inducing growth in carrot root tissue.
固體
胚乳的部分純
化濃縮物,對胡蘿卜根組織的誘導生長也是有用的。
The changing tendencies of the relative contents of phosphorous contained substances have been detected by in - vivo " p magnetic resonance spectroscopy ( in - vivo " p mrs ) during the whole hatching process. in - vivo ] p mrs proved the catabolism of adenosine 5 ' - triphosphate ( atp ), phosphorous ester and phosphocreatine ( pcr ) when the embryo dead. the results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed
用活體核磁共振定域氫譜( in - vivohmagneticresonancespectroscopy , in - vivohmrs )對
胚胎發育過程中羊水和蛋白、蛋黃的成分進行了分析;用活體磷譜( in - vivo 』 』 pmrs )的方法分析了在整個
胚胎發育過程中含磷代謝物的相對含量隨時間的變
化,表明了磷脂類物質及三磷酸腺苷( atp ) 、磷酸肌酸( pcr )在此過程中的變
化及可能的相互轉
化的趨勢,
胚胎死亡后的磷譜也證明了磷脂類物質及三磷酸腺苷( atp ) 、磷酸肌酸( pcr )在死亡過程中降解為無機磷的現象。
Two types of nondepolarizing - activated calcium channels on the spinal cholinergic neurons from embrynoic xenopus laevis
爪蟾
胚胎脊髓膽堿能神經元上兩類非去極
化激活的鈣通道
The origin and evolution of land plants was an important event in the history of earth life and has affected all other lives on the earth and global environment. during the past two decade, the new discoveries of fossil plants microfossils and megafossils from the mid - ordovician through all silurian to the lower devonian improved knowledge of the origin of land plants, provided a time framework of the basal groups for the land plants and the early evolution diversity of vascular plants. three new plant based epochs have been recognized. on the other hand, molecular sequence studies have provided insights into the phylogeny and early branches of land plants. a phylogenetic tree has been established by the joint of a study of comparative morphology and gene sequences. this paper summarizes recent advances and new knowledges, comments on the phylogenetic studies based on the cladistic analysis
陸生植物的起源和演
化是地球生命中的重大事件,它影響到地球上所有的其他生命和全球環境。在過去的20年中,從中奧陶世歷經整個志留紀至早泥盆世的巖層中,
化石植物微
化石和大
化石的新發現改變了人們對陸生植物起源的認識,並且為陸生植物和維管植物早期演
化分異提供了基部類群分
化的時間框架。據此人們識別出地史中的3個陸生植物的時代:始
胚植物時代始維管植物時代和真維管植物時代。
Ranging from 100 to 200 microns across, the width of several human hairs, these microscopic fossils are surprisingly complex and constitute almost a textbook example of a bilaterian, including the three major tissue layers ( the endoderm, mesoderm and ectoderm familiar from high school biology texts ), the presence of a gut with a mouth and anus, and paired coeloms ( body cavities ) surrounding the gut
這些微
化石的大小為100 ~ 200微米,相當于幾根頭發的寬度, ?們看起來出奇復雜,而且簡直就像兩側對稱動物的教科書般,展示了各種特徵:包括三個主要的組織層(內
胚層、中
胚層及外
胚層,都是中學生物課本中熟悉的名詞) ,前後連介面部與肛門的腸腔,腸腔外還圍繞著成對的體腔。
Observation by paraffin section showed that lipeng no. 2 embryo sac mother cells were almost entirely degenerated during megasporogenesis
切片觀察發現,麗? 2號
胚囊母細胞在四核期已完全退
化。
Prevention and cure of the derzsy ' s disease depended on vaccine and antiserum, antibodies of eggs. the vaccines includes goose embryo and duck embryo vaccines which were used 1n breed goose and goslings, and those vaccines have great effect in breeding goose, but the entire virion live vaccines and attenuated vaccines exist many deficiencies. such as preclinical infection, dissemination of virus, recovery of viru5, etc. those proplems can be sol / ed by producing genetic engineering " asclne
目前使用的疫苗分為種鵝用和雛鵝用的鵝胚和鴨胚化疫苗,這些疫苗在實際生產中發揮巨大作用,因其為全毒苗或弱毒苗,存在著潛伏感染,排毒散毒,毒力返祖的缺陷,基因工程疫苗的研製可解決上述問題。