胚卵期 的英文怎麼說
中文拼音 [pēiluǎnqī]
胚卵期
英文
ovigerm stage-
The effect of blastomere damage rate on the clinical outcome in frozen - thawed embryo transfer cycles
卵裂球受損對凍融胚胎移植周期臨床結局的影響Abstract : embryos at different developmental stages are obtained by transplanting ? xenopus laevis blastomere unclei to ? bufo raddei unfertilized eggs
文摘:將非洲爪蟾胚胎細胞核移入花背蟾蜍成熟未受精卵后,得到了發育至各期的胚胎。The fertilized egg entered the 4 - cell stage in 2hlomin, and the 8 - cell stage in 2h20min. the fertilized egg entered the 32 - cell stage in 3hl2min, and the cells distributed in different layers. the fertilized egg entered the multi - cell stage in 4h05min and the early blastula stage in 5h
1小時30分受精卵開始第一次分裂; 2小時10分進入4胞期; 2小時20分進入8胞期: 3小時12分進入32胞期,細胞出現分層現象; 4小時05分進入多細胞期; 5小時進入高囊胚期; 6小時50分進入低囊胚期。Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵,實驗結果如下: ( 1 )顯微注射后,根據胚胎發育分期,胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光,原腸期發熒光的胚胎比例為25 ,後期發育熒光率逐漸下降,肌肉效應期后又相對穩定。Protein kinase a is camp - dependent kinase, one of the most important signal transduction pathways, plays a pivotal role in growth, differentiation, tumor occur, cell cycle control, etc. pka activators such as camp, 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes, also in xenopus oocytes
蛋白激酶a ( proteinkinasea , pka )是依賴于camp的絲蘇氨酸蛋白激酶,是重要的信號傳導途徑之一,廣泛參與許多生命過程,包括生長、分化、腫瘤發生、細胞凋亡、細胞周期調控等。以小鼠卵母細胞為實驗對象,給予pka激動劑camp 、 8 - br - camp或磷酸二酯酶抑制劑imbx ( isomethylbutylxanthine )均可抑制小鼠卵母細胞的胚泡破裂及減數分裂成熟。Docytes are formed during intrauterine life.
卵母細胞是在胚胎時期形成的。The mice showed also ageing - associated decline in oocyte quality, as indicated by increased frequency of premature chromatid separation, spindle disruption and / or chromosome misalignments in mii oocytes of aged mice. the gv oocytes from aged mice normally matured in vitro. the percentages of pronuclear - stage embryos, 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice, but pronuclei formed 1 h later in aged mice than in young mice
老齡小鼠gv期卵母細胞能正常體外成熟;老齡小鼠m期卵母細胞經體外受精后形成原核期胚、 2 -細胞期胚和囊胚的比率與年輕小鼠比較無顯著差異,但原核形成時間延遲1小時;在老齡小鼠卵母細胞,注射牛精子抽提物或sr ~ ( 2 + )處理時所誘發的ca ~ ( 2 + )振蕩以及在減數分裂成熟及早期胚胎中的pkc分佈未發生改變。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件,以hprt為陽性對照,用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析,結果發現,轉錄sry基因的睪丸組織以及雄性胚胎,從受精卵發育到囊胚的過程中,基本上不轉錄xist基因;不轉錄sry基因的雌性卵母細胞和雌性胚胎,從出現原核開始,到發育至2 -細胞期的過程中, xist基因一直不轉錄,但是,從4 -細胞期開始,一直到孵化前囊胚階段,雌性胚胎都轉錄xist基因。There were no significant difference in fusion rate, cleavage rate and blastocyte development rate with different enucleation time of oocytes during ivm16 - 24h ( p > 0. 05 )
在體外成熟16 24h期間,水牛卵母細胞去核時間的不同對核移植后的融合率,分裂率和囊胚發育率沒有顯著影響。The local recent years also have opened the exhibition this research in a handful of laboratory. to find out implatation rate, prenancy rate and multiple - gestation pregnancy rate of blastocyst transferred, we adopted sequential culture system method in this study, 30 cases were performed blastocyst transferred and 30 cases were performed cleavage stage embryos transferred
本實驗採用序貫培養的方法,對30例ivf - et的患者進行囊胚培養及移植,並與30例在胚胎卵裂期移植的ivf - et患者作配對研究,以探討囊胚培養及移植的種植率、臨床妊娠率及多胎妊娠率。Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control
目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響,探討內毒素影響體外受精結局的環節及可能的機制,確定三個體外培養系統對內毒素的敏感性,以期為臨床和實驗室質量控制提供參考。Effects of different electrofusion parameters on activation and early development of mouse embryos reconstructed by cumulus cell nuclear transfer
不同電融合條件對小鼠卵丘細胞核移植重組胚融合和早期發育的影響Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明: ( 1 )脂質體包裹外源基因轉染精子的方法,可將外源基因導入受精卵中,能夠獲得轉基因動物,並得到了較高的轉基因陽性率; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。Obesity, pre - diabetes and diabetes can affect ejaculation, sperm production, ovulation, miscarriage rates and increase fetal malformation rates
肥胖、糖尿病前期和糖尿病都能影響射精、精子產生、排卵、流產幾率和增加胚胎發育不良的機會。Materials all the oocytes and embryos were recruited from reproductive medical center in the first affiliated hospital of zhengzhou university during a five - month period from april 2002 to august 2002. all couples included in this study gave written consent to experimentation on their excess or discarded oocytes or embryos. 1
Et妊娠結局的關系及傲光在輔助解出中的應用研究對象所有研究材料均來自鄭州大學第一附屬醫院生殖中心2002年4月一2002年8月間進行的ivf一et周期;所有用於研究的捐贈或廢棄的卵子胚胎均經患者知情同意。Objective : l. to measure the levels of basal serum follicle stimulating hormone ( fsh ), luteinizing hormone ( lh ), estradio ( e2 ) in peripheral blood in natural menstrual cycle and to investigate the correlations between the hormones and ivf - et outcome. 2. to measure the levels of fsh lh e2 in peripheral blood in controlled ovarian hyperstimulation ( coh ) cycle and to investigate the correlations between the hormones and ivf - et outcome
目的: 1 、測定控制超排卵患者控制超排卵周期前基礎外周血促卵泡素( fsh ) 、黃體生成素( lh ) 、雌二醇( e _ 2 )水平,並探討其與體外受精-胚胎移植( ivf - et )結果的相關關系。Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ,分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集,其餘胚胎的染色體則處于原核期;而只在融合后3h , 27 . 9 %重構胚被標記出組裝的紡錘體,且其中的同源染色體己經分離。It contained 187 amino acids deduced from the nucleotide sequence, and showed 65 % amino acid sequence identity with human aoeb166. the analysis of n - and c - terminal domains revealed amino acid sequences characteristic of features of mitochondrial and peroxisomal targetin
原位雜交分析表明從受精卵到神經胚, aophihmgb基因廣泛表達,但隨著發育的進行,中胚層、內胚層和神經管的表達減弱,到1天幼蟲期,僅在表皮可檢測到表達。The development process, from fertilized egg to spiracular formation can be divided into 26 stages, took 212. 94 hours under the water temperature of ( 20 0. 5 ) and 170. 95 hours under the natural temperature ( 21. 5 24 ). the external morphological characteristics in various stages of the embryonic development were similar to those of rana nigromaculata, but the hatching stage was later than that of rana nigromaculata and earlier than that of rana limnocharis
自受精卵期至鰓蓋完成期共分為26個時期,其發育歷程及各時期胚胎外形特徵與黑斑蛙基本相似,但孵化期比黑斑蛙推遲三個胚期而早於澤蛙,在水溫( 20 0 . 5 )及常溫( 21 . 5 24 )條件下,其胚胎發育的全時程分別為212 . 94小時和170 . 95小時。Gonadal development and fecundity are affected by certain essential dietary nutrients, especially essential amino acids, essential fatty acids, and vitamins etc. thus, during the last two decades, more attention has been paid to the optimal level of different nutrients in broodstock diets. it is well known that protein is a kind of indispensable nutrient because of its important physiological function on body structure and supplying energy to the growth and development of shrimp and crab. protein and amino acids of broodstock diet have been identified as major dietary factors that determine successful reproduction and survival of offspring
而這些問題的解決則要求對中華絨螯蟹生殖過程中的攝食和消化生理機制,親體性成熟期對各種必需營養素(如必需氨基酸、必需脂肪酸和維生素等)的需求,卵細胞發生過程中營養物質的合成、轉運及貯存規律,以及在受精卵、胚胎和后續以內源性營養為主的?狀幼體發育階段對卵黃磷蛋白的消耗和利用模式等環節進行較為深入的研究,在全面系統地了解中華絨螯蟹的生殖營養生理的基礎上,探討親體生殖營養對卵質、孵化及后續幼體生長發育的影響。分享友人