胚囊外的 的英文怎麼說
中文拼音 [pēinángwàide]
胚囊外的
英文
extra-saccal-
According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere
摘要從哺乳動物早期胚胎體外培養發育阻斷機理和早期胚胎的體內環境入手,闡述了胚胎體外培養各細胞階段胚胎對營養物質的需求,尋求合理的體外培養條件和方法,以便提高體外胚胎早期的囊胚發育率。Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded
方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份,分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應,應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率;小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素,觀察受精情況並記錄受精率;取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎,與不同濃度內毒素共孵育進行體外培養,觀察體外發育情況並記錄囊胚率。The mice showed also ageing - associated decline in oocyte quality, as indicated by increased frequency of premature chromatid separation, spindle disruption and / or chromosome misalignments in mii oocytes of aged mice. the gv oocytes from aged mice normally matured in vitro. the percentages of pronuclear - stage embryos, 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice, but pronuclei formed 1 h later in aged mice than in young mice
老齡小鼠gv期卵母細胞能正常體外成熟;老齡小鼠m期卵母細胞經體外受精后形成原核期胚、 2 -細胞期胚和囊胚的比率與年輕小鼠比較無顯著差異,但原核形成時間延遲1小時;在老齡小鼠卵母細胞,注射牛精子抽提物或sr ~ ( 2 + )處理時所誘發的ca ~ ( 2 + )振蕩以及在減數分裂成熟及早期胚胎中的pkc分佈未發生改變。We transferred the hetero - logous gene into toreniafournieri successfully. this method laid a foundation for study of double fertilization of angiosperm by transgenic tecnology
我們將外源基因成功地導入藍豬耳,為利用轉基因的手段研究被子植物受精和胚囊發育打下基礎。- 10 - ivm oocytes were fertilized in vitro, and the process of fertilization, early cleavage, and blastocyst formation were recorded
通過體外受精觀察比較各組體外成熟卵細胞的受精率、卵裂率和囊胚形成率: 6There were no significant difference in fusion rate, cleavage rate and blastocyte development rate with different enucleation time of oocytes during ivm16 - 24h ( p > 0. 05 )
在體外成熟16 24h期間,水牛卵母細胞去核時間的不同對核移植后的融合率,分裂率和囊胚發育率沒有顯著影響。In order to improve the cuture system of mammal embryo and advance the quality of embryo in vitro, the effects of taurine and ethylenediaminetetraacetic acid ( edta ), estrogen ( e2 ) and progesterone ( p ) on preimpiantation development of mouse embryos in vitro were examined and the quality difference of blastocysts from in vitro and in vivo by blastocyst staining and embryo transfer was evaluated
為完善體外培養體系,提高體外發育的胚胎質量,本實驗研究了牛磺酸( tau ) 、 edta 、雌激素( e2 )和孕激素( p )對昆明白小鼠胚胎體外發育的影響。在此基礎上,通過胚胎移植技術觀察了不同來源的囊胚在移入子宮后的發育情況,以比較體外培養和體內發育的囊胚質量。To select the best mouse blastocysts resulting from mm16, mm16 + e2, mm16 + p and mm16 + e2 + p mediums, and from ivf - ivc ( in vitro fertilization and in vitro culture ) in htf medium, and from in vivo was for embryo transfer
分別選取小鼠1 -細胞胚胎在mm16 、 mm16 + e2 、 mm16 + p 、 mm16 + e2 + p各組培養液中培養的囊胚、小鼠體外受精-體外培養的囊胚和體內正常發育的囊胚,進行子宮內移植。The blastocyst culture and transfer is a big progress of the under medical treatment in ivf - et in near period. on abroad many the center of ivfs have been used as this the routine technique proceed the application
囊胚培養和移植是近期ivf - et治療中的一大進展,在國外的許多ivf中心,已將這作為常規技術進行應用。Recovery of microtubule organizing centers in enucleated oocytes and condensation of chromosome and organization of spindle in reconstructed embryos were analysed. a non - invasive method of enucleation mouse oocytes has been developed. firstly, the progression of mouse oocytes in meitosis i was ascertained by immunofluoresence technique
另外,本試驗通過在m16基礎上添加eaa 、 non - eaa和葡萄糖顯著提高了胚胎的桑椹率、囊胚率和孵化率;單獨添加葡萄糖的培養液,其桑椹率顯著升高,但囊胚率和孵化率的升高並不顯著,所以胚胎培養液中氨基酸和葡萄糖能協同促進胚胎發育。Since the first embryonic stem cell derivation from mouse blastocyst by british scientists in 1981, groups of scientists were devoted into the research field of stem cells. in 1998, two scientific groups of america obtained human embryonic stem cells and human embryonic germ cells respectively from human blastocysts and gonadal ridge of early embryos
1998年,美國的兩個科學家小組分別從人體外受精發育而成的囊胚內細胞團和5 9周齡流產的胚胎原始生殖嵴中,成功獲得了人類胚胎幹細胞系和人類生殖嵴幹細胞系。Based on these observations, we isolated protoplasts from female germ unit and performed extracellular ca2 + influx experiment in order to check whether the cytological event similar to what happens during fertilization will be induced by extracellular ca2 + influx
表明細胞壁的合成是受精后早期發生的細胞學事件。在此基礎上,我們分離了胚囊細胞的原生質體。通過外源導入鈣離子檢查鈣能否引發類似受精過程發生的細胞學事件。The results showed that es cells derived from kunming albino mouse remained undifferentiated state when they were cultured in vitro for a long time, and karyotypies were stable, but the ability of germ - transmission have not been verified
結果顯示,用昆明白小鼠囊胚所建立的胚胎幹細胞在體外長期培養時仍能保持未分化的狀態,核型穩定,但還未證明其具有生殖系嵌合的能力。A low implantation potential of human embryos replaced in the uterus after in vitro fertilization ( ivf ) is a great barrier in assisted reproduction. recently, failure of the embryonic zona pellucida to rupture following blastocyst expansion has been supposed as a possible contributing factor in implantation failure. and then an idea of creating a hole artificially in the zona pellucida, i. e., assisted hatching ( ah ) has been put forward in order to help blastocyst hatching
體外受精胚胎移植( invitrofertilization - embryotransfer , ivf - et )中胚胎種植潛力較低一直是困擾人們的一個問題,近年來的一些研究認為囊胚擴張后透明帶不破裂可能是種植失敗的一個原因,於是提出人為地在透明帶上打孔幫助胚胎孵出,即輔助孵出( assistedhatching , ah )的思想。Although the cell parameters ( icm and tcn ) of blastocysts of both groups differed significantly ( p < 0. 05 ), the mml 6 + e2 + p group was the closest to the in vivo group, and those of the mm16 group were the lowest
同時添加e2和p的mm16培養液中囊胚的細胞總數、內細胞團數、內細胞團占總細胞的百分率和內細胞團與滋養層細胞數的比率低於體內正常發育囊胚,但顯著高於其它體外培養組( p 0 . 05 ) 。The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin. the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo. angiostatin showed significantly inhibitory effect on endothelial cells
重組病毒以moi = 10感染家蠶細胞( 2 10 ~ 6個細胞/ 5ml )和家蠶5齡幼蟲,表達產物用體外培養的人臍靜脈血管內皮細胞( ecv304 )及體內雞胚尿囊膜( cam )新生血管實驗檢測其抑制活性: (一)血管抑素可明顯抑制體外培養的內皮細胞增殖。分享友人