胚性幹細胞 的英文怎麼說
中文拼音 [pēixìnggànxìbāo]
胚性幹細胞
英文
embryonic stem cell-
Purpose embryonic stem cells ( escs ) which are from the inner cell mass ( icm ) of preimplantation blastula are pluripotent. neurons are considered as terminally differentiated cells that can not divide nor proliferate after injury
目的胚胎幹細胞( embryonicstemcells , escs )來源於著床前胚胎的囊胚期內細胞團( innercellmass , icm ) ,具有發育全能性。Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell
小鼠限制性片段長度多態性dna指紋圖jl - 02探針胚胎幹細胞Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells. in vitro, es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers
胚胎幹細胞( embryonicstemcell , escell )系是從早期胚胎細胞分離培養而建立的高度未分化的具有全能性的細胞系。Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料,經傳代培養,獲得能連續而穩定傳至6代的,具有胚胎幹細胞諸多特性的eg細胞系。Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術,檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明:採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜,具有足夠的多態性和很好的穩定性。Embryonic stem cells ( esc ) are undifferentiated totipotent cells that could be differentiated in vivo into any kind of cells theoretically, including germ cells
摘要胚胎幹細胞是一種具有發育全能性的細胞系,理論上能分化為包括生殖細胞在內的各種組織細胞。One issue in using uniparental es cells for tissue transplants is their durability and safety
使用單系胚胎幹細胞進行組織移植中的問題便是其耐用性和安全性。According to the decision made by the opposition division, researches relating to human embryonic stem cells pertaining to the use of human embryos for industrial or commercial purposes as stipulated in rule 23d ( c ) epc and should not be made patentable
根據歐洲專利局異議部門之決定,人類胚胎幹細胞之研究屬于歐洲專利公約施行細則23d ( c )所規定之人類胚胎商業性利用,因此屬違反公序良俗者而不予專利。In the third experiment, we first constructed a test model of differentiation from mouse es cells to cardiomyocytes. then series of dilutions of three chemicals were screened on this platform. the combination of all trans retinoic acid and dmso is employed as positive control
實驗三首先建立了一個小鼠胚胎幹細胞定向分化成心肌細胞的實驗平臺,然後採用了3種心肌疾病相關的藥物:黃芑生脈飲、 1 , 6二磷酸果糖和hg (代號) ,在此定向分化的實驗平臺上進行了一系列濃度的藥物篩選,實驗採用視黃酸和dmso的混合誘導劑作為陽性對照。To carry out basic researches in mainly three fields, including human embryonic stem cell researches, preimplantation genetic diagnosis researches or female reproductive tissue banking researches
主要開展人類胚胎幹細胞、種植前胚胎遺傳學診斷以及女性生殖組織庫的相關基礎研究工作。Hundreds of combinations of different types of antigens are possible, meaning that hundreds of thousands of es cell lines might be needed to establish a bank of cells with immune matches for most potential patients
不同的抗原類型可能有數百種的組合方式,也意味著這可能需要建立一個含有數十萬種胚胎幹細胞株的細胞庫,以比對出與患者免疫相容性最高的幹細胞。The lines generated, though, do appear to be pluripotent
胚胎幹細胞群可以表現出來細胞多能性。Es cells ' pluripotency, however, makes this far too dangerous an approach for human therapy
不過,由於胚胎幹細胞的多能性,使得這種方法太危險,不適用於人體治療。Study on the differentiation potential of epidermal - like stem cells derived from human embryonic stem cells
人胚胎幹細胞源性表皮樣幹細胞分化潛能Here we report a simple and effective method to induce escs into neurons, and with the help of genechip analysis we can confirm the induced cells as neurons and find some useful or specific genes in neural conversion as well
本實驗擬摸索出一種簡便有效、無毒性的體外大比例誘導鼠胚胎幹細胞分化為神經細胞的方法,並進一步通過基因晶元分析證實誘導結果,篩選神經細胞分化主導基因。Gene expression of neural related genes was identified by semi - quanti - tive rt - pcr analysis and genechip assay. 4 and 10 days after neural induction, gene expression pattern was analysed by genechips, which showed the expression of some neural stem cells and mature neurons specific and related genes, repectively. especially the expression of gabar and glutamate dehydrogenase ( gad ), which meant the induced cells could be gabanergic neurons
2 .基因晶元檢測到未分化escs 、神經幹細胞及成熟神經細胞的相關基因,並由半定量rt一pcr證實基因晶元檢測未分化細胞表達胚胎幹細胞特異基因;誘導后第4天細胞高表達神經幹細胞特異性基因;誘導后第10天細胞高表達成熟神經細胞特異性基因,且有gaba受體、谷氨酸脫梭酶( gad )表達,說明誘導后細胞大多為以ba能神經元。In addition, the bone marrow - deried stem cells, which could be differentiated into neurons and other cells making up the central nerous system, spread far and wide and behaed like neural stem cells when transplanted into the brain tissue of chicken embryos
除此以外,骨髓源性幹細胞能夠分化為神經元和其它細胞共同構成中樞神經系統,當其植入到小雞胚胎的腦組織中後分布非常廣泛,其特性同神經幹細胞類似。Inherited gene mutations, such as those that cause hemophilia or muscular dystrophy, would have to be corrected as well before using a patient ' s own cells to create es cells
在使用帶有遺傳性基因突變(例如血友病或肌肉營養性萎縮癥)患者的細胞來製造胚胎幹細胞之前,也需要先矯正缺陷基因。In these years, because of its totipotency, manipulation and plastics, the embryonic stem ( es ) cells have been the focus of researches in the fields of medical and life science
近年來,胚胎幹細胞( escells )以其具有全能性、可操作性和可塑性等諸多優點,已經成為醫學和生命科學領域研究的熱點之一。分享友人