胚性細菌 的英文怎麼說
中文拼音 [pēixìngxìjūn]
胚性細菌
英文
embryonal cell-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in 6 live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 " c for 72 hours. hi and nl assays were performed to detect the subtype of viruses if ha test were positive ( ad ^ 16 ) and contamination test were negative
材料和方法：每星期收集一次標本，收集的標本常規處理， 9 - 11日齡雞胚尿囊腔接種， 35培養72小時，收取尿囊液，血凝陽性（ ad 16 ）且細菌培養陰性者- 70保存並進一步做hi ， ni實驗鑒定亞型。
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