胚發生培養 的英文怎麼說

中文拼音 [pēifāshēngpéiyǎng]
胚發生培養 英文
embryogenetic culture
  • 胚 : 名詞[生物學] （初期發育的生物體） embryo
  • 發 : 名詞（頭發） hair
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  1. For long-term tissue cultures of domestic carrot the only requirement for adventive embryogeny was addition of an auxin.

    栽培的胡羅卜進行長期組織培養時，對不定胚發生的唯一要求是加進植物生長素。
  2. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  3. Efficiency of embryogeny in isolated microspore culture of head cabbage

    結球甘藍游離小孢子培養胚發生能力的研究
  4. Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed

    摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。
  5. Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays. this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content. the calli of the transformed sublines a78 - 3, a78 - 4, a78 - 5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established

    以轉基因亞系a78 - 3 、 a78 - 4 、 a78 - 5和未轉基因對照a95 : 88 : 22的細胞愈傷組織為實驗材料，以dkm和lp - m熟化培養基培養五周后，再以1 4sh萌發培養基培養四周，成功地誘導形成了胚胎，並再生成新的小植株，萌發成活率達到80 。
  6. Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

    建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
  7. With the increase of substrate salinity, the accumulated sodium and chloride increased. as a result, all tissues had considerablly lower osmotic potentials than that of the solution on which they were grown at 60 day after planting. changes in length, dry weight, water content, ion concentrations, osomotic potential, ion content of hypocotyls during culture indicated that viviparous hypocotyls not only afforded nutrition for seedling growth, but also reserved ions, thus charged the balance of ion concentration and osmotic potential of the seedling

    鹽脅迫下幼苗單株葉面積下降的程度大於光合速率的降低，葉面積的減小是導致減產的主要原因；木欖幼苗各組分中的離子濃度以及含量隨栽培時間而變化；栽培初期剛萌根時，幼苗原胚軸中的離子滲漏到培養液中；此後隨著根系的發育以及芽的生長，幼苗轉為從培養液中吸收離子，並以吸收na 、 cl離子為主。
  8. No mature seeds were used the method of embryos culture. during culture of the hybrid embryos form different cross classification, the proportion and concentration of hormones were different

    對早培育胚進行胚培養發現，不同的雜交組合，雜種胚生長所需的激素濃度和配比不同。
  9. Techniques in embryo technology ( such as in vitro production of embryos and animal cloning ) need large quantities of high quality oocytes. but the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. it is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo

    目前胚胎工程技術研究和開發（如體外生產胚胎和體細胞克隆等）需要大量高質量的成熟卵母細胞，但利用屠宰動物卵巢卵母細胞經過體外成熟培養而獲取的卵母細胞質量還遠不如體內成熟卵母細胞，其原因一般認為是由於缺乏體內主卵泡階段的獲能作用。
  10. No production by mouse embryos embryos were cultured in hanks balanced salt solution for 4 hours. then the culture medium was collected, and equal amount of griess reagent was added into it. no concentrations were determined indirectly by spectrophotometry

    胚胎發育過程中no的生成胚胎在hanks液中培養4小時后，取培養液加入等體積的griess試劑，用分光光度法檢測培養液中亞硝酸鹽的濃度，依此得知no的生成。
  11. In vitro infertilization - embryo transfer ( ivf - et ) development up to now, there are many new breakthroughs in coh ( controlled ovarian hyperstimulation ), the embryo culture technique and improvement of the culture medium, but how to increase the lower embryo implantation rate, clinical pregnancy rate, and reduce multiple pregnancy rate to perplex to reproduction medicine worker always

    前言體外授精?胚胎移植技術（ invitroinferfilizafion - embryotransferivf - et ）發展至今，在促排卵方案，胚胎培養技術及培養液的改進方面有了許多新的突破，但如何提高較低的胚胎種植率及臨床妊娠率，始終困擾著生殖醫學工作者。
  12. Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body

    將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。
  13. In this paper, the progress of isolated microspore culture was reviewed : the major factors affecting regeneration ; the methods of reduplication ; the major factors affecting the formation of embryo, for example, experimental material, culture medium, raise condition, a phase of microspore growth

    通過對影響大白菜游離小孢子胚胎發生的主要因素材料基因型、供體母株生長狀態、小孢子發育時期、培養方法、培養條件和影響植株再生頻率的因素以及小孢子植株的加倍方法等進行了綜述。
  14. Furthermore, the scientists were also able to grow progenitor blood cells in culture from uniparental es cells, and upon transplant into irradiated adult mice, show that these cells contribute, long - term, to the function of their hematopoietic system

    此外，科學家還能夠利用單系胚胎幹細胞在培養基中培育血液祖細胞，在移植入輻射照射的成年小鼠后，發現這些細胞對造血系統功能產生長期的作用。
  15. Sweetpotato pollens killed by u. v. didn ' t sprout ; 2. normal pollens sprouted ; 3. pollens of 5x mixed with recognition pollens attached and sprouted much ; 4. in the negative - cross, sweetpotato pollens attached and sprouted much on the stigma of 5x ; 5. in the possitive - cross without recognition pollen, 5x pollens few attached and sprouted ; 6. in the treatment of pgr ( twice ), globular - embryo observed on 15 days after pollination ; 7. ovule obtained by intercross germinated on the medium ; 8. plantlet from intercross ovule grew on the medium ; 9. seeds obtained by opening pollination ; 10. tubers of hybrids from 5x crossed by sweetpotatos for two generations

    紫外線殺死的甘薯花粉在親和柱頭上不萌發； 2 .未經紫外線處理的甘薯花粉在柱頭上正常萌發； 3 .在蒙導花粉作用下，五倍體的花粉在甘薯柱頭上大量附著和萌發； 4 .反交組合甘薯花粉在五倍體柱頭上大量附著和萌發； 5 .正交組合無蒙導花粉時五倍體花粉少量附著和萌發； 6 .生長調節劑二次處理后，授粉后15天所見的球形胚； 7 .雜交胚珠在培養基上萌發； 8 .雜交胚珠培養成苗； 9 .放任授粉收獲的大量種子； 10 .五倍體與甘薯雜交兩代產生的後代群體的結薯性。
  16. At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines

    繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。
  17. The embryo of these two varieties can grow into healthy plantlet after being transferred to medium with no hormone

    兩品種愈傷組織上發生的胚狀體接種到不含激素的基本培養基上后都能長成健康小植株。
  18. Suitable hormone and cytokin concentration as well as time for excising ovary were crucial for increasing the number of hybrid embryos and the pecentage of hybrid embryos

    在培養中，適宜的生長素和細胞分裂素濃度以及恰當的子房離體前的發育時間能提高成胚數和成胚率。
  19. Via suspension culture and 0. 5 mol l ra induction, we established an in vitro system to differentiate es cells directly into neuron - like cells. in this system, neuron - like cells first appeared within 48h after the embryoid bodies were plated, and were most abundant 4 - 6 days after plating, while decreased 8 - 10 days later. neuron - like cells differentiated from es cells had typical morphologic properties, and specifically reacted with nf antibodies

    通過懸浮培養和0 . 5mol l ra誘導，建立了定向誘導es細胞向神經元樣細胞分化的體系。在該體系中，神經元樣細胞于類胚貼壁后48h之內出現， 46d時達到最多， 810d后減少。由es細胞分化而來的神經元樣細胞具有典型的形態特徵，並且可以與nf抗體發生特異反應。
  20. It was also noted that irradiation treatment with low dose ( 10gy ) of y rays exerted slight stimulating effects on callus induction and formation of embryogenic calli in particular. production of embryogenic calli was obviously promoted by addition of 0. 1 ~ 0. 2mg / l bap or 2. 5mg / l cuso4 5h2o, or enhancement of sucrose concentration to 60g / l in subculture medium

    在繼代培養基中添加0 . 1 0 . 2mg lbap或2 . 5mg l硫酸銅，或將繼代培養基中蔗糖濃度提高到60g l能促進胚性愈傷組織發生，提高胚性愈傷組織頻率。
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