The changing tendencies of the relative contents of phosphorous contained substances have been detected by in - vivo " p magnetic resonance spectroscopy ( in - vivo " p mrs ) during the whole hatching process. in - vivo ] p mrs proved the catabolism of adenosine 5 ' - triphosphate ( atp ), phosphorous ester and phosphocreatine ( pcr ) when the embryo dead. the results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed
用活體核磁共振定域氫譜( in - vivohmagneticresonancespectroscopy , in - vivohmrs )對
胚胎發育過程中羊水和蛋白、蛋黃的成分進行了分析;用活體磷譜( in - vivo 』 』 pmrs )的方法分析了在整個
胚胎發育過程中含磷代謝物的相對含量隨時間的變化,表明了磷脂類物質及三磷酸
腺苷( atp ) 、磷酸肌酸( pcr )在此過程中的變化及可能的相互轉化的趨勢,
胚胎死亡后的磷譜也證明了磷脂類物質及三磷酸
腺苷( atp ) 、磷酸肌酸( pcr )在死亡過程中降解為無機磷的現象。
It " s shown from the results that the pituitary gland of silurus asotus originates from two embryonic cells of different position ; and the doral wall of the original cavum oris forms the rostral pars distali ( rpd ) and the proximal pars distalis ( ppd ) ; the infundibulum of the ventral part of diencephalons forms the the pars intermedia ( pi ) and the neurohypophsis
結果表明:鯰腦垂體由兩個不同部位的
胚胎細胞形成,原始口腔背壁外
胚層分離出來的細胞構成
腺垂體的前外側部( rpd )和中外側部( ppd ) ,從間腦腹面漏斗體分離出來的細胞構成
腺垂體中間部( pi )及神經垂體( nh ) 。
( 3 ) the chromosomes obtained by air - drying and chromosomes from the silkworm genital gland are similar in shape. but the numbers and the length of the chromosomes are obviously different. the chromosomes obtained from the genital gland have little difference in length, while the chromosomes of bmn cell are different in length very much
但是,在染色體的數目上有顯著的差異。另外,染色體間的長短差別也非常明顯。由家蠶生殖
腺得到的減數分裂中期染色體和
胚胎得到有絲分裂的中期染色體,其染色體長短差異不明顯,而binn細胞的中期染色體長短非常不一致,差異非常顯著。
The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性:雞
胚尿囊液經離心、磷鎢酸負染后,電鏡觀察該病毒為典型的冠狀病毒;該毒株的第一代尿囊液對雞
胚無肉眼可見的致病作用,當繼代到第5代后,
胚體嚴重病變;病毒在雞
胚中隨著接種時間的延長,其效價增高, 96h可達到48h的2倍;該毒株可在cef上生長,但不能形成明顯的蝕斑;經1胰酶處理后可凝集雞紅細胞;雞
胚的第四代尿囊液病毒回歸動物體,病死雞腎臟呈典型的花斑腎,
腺胃則未見肉眼可見的病變。
Effect of higher boron on post embryonic development of thymus in gu - shi chicken
高硼對固始雞胸
腺胚后發育的影響
The steady dead generation and time that was caused by the isolated virus was certain by chicken embryo which was inoculated on seven or nine days. the histopathological changs of the infectious stunting syndrom were studied by the way of ordinary paraffin section and he dying. the experimental result were as follows : the test proved that the changes of the chicken embryo were different in different stage. the chicken embryo dead in a week after it inoculated. the body was dropsy and hemorrhage. dead before it hatched out, the embyo body were dropsy, pale and slime. the liver was yellow and swolled, gallbladder ( vesica fellea ) was filled with bile. bursa and glandula thymus analosis. the kindey dropsy. bowel lamina were humble, dilatation. gas and yellow foam were filled the bowel. histopathological changes were that, in early stage, obvious changes of liver and kindey were dropsy, hemorrhage and necrosis. two types eosinophilic intranuclear inclusion bodies including large round and little granular were present in cells of the above organs. the obvious changes of bursa were dropsy, adverse folliiculated growth and little lymphocytes proliferating, 19 - 21 days chicken embryo, one or two big empty vacuoles were prensent in cells of liver and kindey. the number of the folliculi was growing, the vacuoles between cells were larger
膽囊充盈、其內充滿稀薄的膽汁;法氏囊、胸
腺萎縮,腸道擴張、腸壁菲薄、內充滿氣體及黃色泡沫狀物;腎臟腫大。病理組織學變化方面,早期肝臟、腎臟、腸主要以出血、水腫和壞死為主,且肝細胞核及腎小管的上皮細胞核內均發現有核內包涵體,包涵體呈嗜酸性,為大型圓形包涵體或不規則的顆粒狀;法氏囊則以水腫、濾泡發育不良、小型淋巴細胞數量增多為主。 19 21日齡雞
胚肝細胞、腎小管上皮細胞的胞漿內出現1 2各大的空泡,法氏囊濾泡數目增多細胞間有較大空隙。
After thymus transplantation, the embryo ' s thymus has trunk cell that comes from the embryo ' s yolk bag, embryo liver and marrow, it excretes a great deal of thymus hormone which change lymphocyte into mature t lymphocyte through multiplication and differentiation, then it help to turn b cell into serous cell, the quantity of the serous cell increases, the antibody that it produces also increases, that is the content of iga, igm and igg is increased
而移制胎兒胸
腺后,由於胎兒胸
腺內有來自胎兒卵黃囊、
胚肝和骨髓的幹細胞,並能分泌大量胸
腺素,促進淋巴細胞在該處增殖、分化為成熟t淋巴細胞,繼而輔助b細胞活化成為漿細胞,漿細胞數量增加,產生的抗體量增加,即igg , igm , iga含量增加。
In addition, insulin - ir cell, chromogranin - a - ir cell, testosterone - ir cell and epithelial membrane antigen - ir cell were not detected in the digestive tract of alligator sinensis embryos. the kinds and the numbers of endocrine cells in the digestive tract and glands were less at early prenatal stages but increased greatly in the midterm of development. in the digestive tract of last prenatal stages, it was stomach that had the most endocrine cells and might play the most important role in regulating the growth and differentiation of the digestive tract, the duodenum was in the second place
縱觀揚子鱷
胚胎消化道和消化
腺內分泌細胞發生的情況,可得出如下結論: )
胚胎發育早期,胰
腺內分泌細胞的數量和種類較多,可能是早期
胚胎重要的內分泌調控部位;
胚胎中期消化道和消化
腺內分泌細胞的數量和種類增加最多,是內分泌細胞分化發育的重要時期:
胚胎晚期,以胰
腺和幽門、十二指腸的內分泌細胞最密集,為孵出后的消化和吸收的功能調控做好生理準備。
The endocrine cells in the digestive and glands of alligator sinensis embryos aged from 8th to 55th day were localized and compared by using immunohistochemical method with thirteen kinds of antiseras of hormone. during the development of pancreas in alligator sinensis embryos, somatostatin ( ss ) immunoreactive ( ir ) cells, 5 - hydroxytryptamine ( 5 - ht ) - ir cells, glucagon ( glu ) - ir cells, epidermal growth factor ( egf ) - ir cells appeared on 18th day. no p53 protein - ir cell, gastrin - ir cell, testosterone - ir cell, chromogranin a - ir cell, vasoactive intestinal polypeptide - ir cell, epithelial membrane antigen - ir cell or insulin - ir cell was found in the pancreas of alligator sinensis embryos
本實驗採用免疫組織化學技術,應用13種不同的抗血清,對孵育時間8 ? 55天揚子鱷
胚胎消化道及消化
腺內分泌細胞的種類進行鑒別、定位和比較,結果如下:揚子鱷
胚胎胰
腺中,生長抑素、 5 ?羥色胺、胰高血糖素、表皮生長因子、胰多肽免疫反應陽性細胞出現于第8天; p物質免疫陽性細胞出現于第18天; p53 、胃泌素、睪酮、嗜鉻素a 、血管活性腸肽、上皮膜骯原、胰島素免疫陽性細胞在各期揚子鱷
胚胎胰
腺中均未發現。
There were strong stat3 immunostaining in the glandular epithelium and weak nuclear translocation of tyrosine - phosphorylated stat3 in the luminal epithelium containing a blastocyst in the day 4 morning
第4天上午子宮
腺上皮中的stat3免疫染色較強,且在
胚泡所在處的的子宮腔上皮中有弱的酪氨酸磷酸化stat3蛋白的核轉位。
Cea gene was transferred into human dcs, and specific anti - cancer effecs induced by the vaccine was observed. this test is part of my tutor ' s. hang you - tian has observed the induction of crcinembryonic antigen ( cea ) - specific cytotoxic t - lymphocyte responses in vitro when he transfected dcs with pcdna3 - cea, and has observed the immunity effects of the dcs ( pcea ) inoculateing against to ct 26 ( hcea + ) loaded in balb / c mice. after vaccination with the cea gene - modified dc, the survival time of the mice vaccinated with ct26 + ( cea + ) ws prolonged more potently than that of the mice vaccinatd with other dcs
癌
胚抗原( carcinoebryonicantigen , cea )是一種研廠鄭州大學2002年碩士畢業論文轉染人癌
胚抗原真核表達質粒的人樹突狀細胞的抗瘤作用究最為深入的腫瘤相關抗原( tumorassociatiednigen , taa ) ,在90的胃腸道腫瘤、 50的乳
腺癌及70的非小細胞肺癌中有高水平的表達,是目前國際上公認的腫瘤標志物。
Intracranial embryonal carcinoma is a rare germ cell tumor found predominantly in the pineal region and, to a lesser extent, in the suprasellar region
摘要顱內
胚胎瘤是一種少見的生殖細胞瘤,而且大多好發于顱內松果
腺區域或上蝶鞍部。
Using anatomical method, the gonadal development in postembryonic period of rana chensinensis, which had been migrated from northeast china, is studied
摘要採用解剖學方法,對南移后的中國林蛙
胚胎後期性
腺發育進行了觀察。
The results showed that the gonadal development in postembryonic period of rana chensinensis was well - balanced, and the environment of guangdong is adapted to the gonadal development
結果表明,南移后的中國林蛙
胚胎後期的性
腺發育基本正常,環境的變化並未對其產生不良影響。
In the day 4 afternoon and midnight, stat3 expression and nuclear translocation of tyrosine - phosphorylated stat3 became stronger in the glandular and luminal epithelium of the potential implantation sites
第4天下午及午夜,
胚胎將要著床處子宮的腔上皮和
腺上皮中stat3表達繼續增強,酪氨酸磷酸化stat3的核轉位明顯增強。
Method of metaphase chromosome preparation from scallop was optimized. the gill tissue and embryo before the stage of trochophoro larve were proved to be good materials for chromosome preparation, while excellent chromosomes can hardly be prepared from the gonad tissue or embryo after the stage of trochophoro larve
確定成體扇貝鰓絲或者擔輪幼蟲期的早期
胚胎是較為優良的染色體制備取材來源,而性
腺材料、進入d型幼蟲期以後的扇貝
胚胎則較難以制備優良的染色體分裂相。
This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是:具有可以調控外源基因在乳
腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區;將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後,而且沒有增加額外的序列和使人血清白蛋白cdna移碼;引入標記基因gfp ,便於在
胚胎期鑒定陽性
胚胎,減少受體;引入cre lox重組系統: ( ? )標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后,刪除標記基因; ( ? )餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
Cea is probably the most extensively characterized human tumor associated antigen. it was discovered in colorectal and pancreatic carcinomas in 1965 and it " s full cdna was cloned in 1987. it is a kind of glycoprotein and it " s molecular weight is 180kd
癌
胚抗原( carcinoembryonicantigencea )是一種最常見的腫瘤相關抗原( taa ) ,也是國際上公認的腫瘤標志物,於1965年首次在人結腸癌和胰
腺癌中發現, 1987年克隆出它的全長基因序列。
In this experiment, the lung cells of embryo sheep were cultured and the cells were infected with jsrv
將綿羊的胚胎肺細胞進行原代培養,接種綿羊肺腺瘤樣品,進行病毒分離。