胞外片段 的英文怎麼說

中文拼音 [bāowàipiānduàn]
胞外片段 英文
extracellular segment
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 外 : Ⅰ名詞1 （外面） outside; external side 2 （外國） foreign country 3 （以外） besides; beyond; in ...
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. We focused on the following aspects ; 1 ) we first assayed the expression of complement receptors and complement - associated molecules on distinct subsets of dendritic cells during their development in order to understand the physical basis of the sensitivity of dendritic cells to complement and its split products ; we next studied the effects of complement activation on the survival of dendritic cells during their development ; and finally examined the effects of the whole complement system, focusing on the ability of one of the split products of complement activation, c5a, and its first subcomponent - c1q, to influence chemotaxis of dendritic cells, as well as allo - t cell stimulatory activity of dc

    我們通過免疫磁珠分離了兩種人dc前體，即髓系來源的單核細胞（ monocytes ， mo ）和淋巴系來源的漿細胞樣dc （ plasmaeytoiddendriticeells ， pdc ） ，對這兩個不同dc亞群進行體外誘導培養，使其處于不同的分化發育階段，然後檢測了其表達補體受體一cd35 （ cri ） 、 cd21 （ crz ） 、 cdilb （ cr3 ） 、 cdlle （ cr4 ） ，補體調控蛋白一cd46 、 cd55 、以及部分補體片斷分子受體一c3ar 、 csar 、 clqrp的水平。
  3. At the surface of the pollen grains there are three types of aperturates, including three porates type, three colpates type and three porate - colpates type ; and four types of sculpture, namely, reticulate, striate, striate - reticulate and verrucate. the upper epidermal cells of the leaves of 12 species plants are detected with hpias - 1000 image analytic system through the treatment of binarization, and the experimental methods are proved stable. the results indicated that the waviness of the anticlinal walls ( sfc ) and the ratio of the feret ' s diameter ( slf ) of the epidermal cells of the middle lamina in the third node of leaves starting from the lowerest part of the stem have a relative constant range and could distinguish from each other. oieanolic acid, l, 5, 8 - trihydroxy - 3 - methoxyxanthone and swertiamarin are separately detected on the tlc, and the different chromatogram of various plants can be considered as characters of identification. the contents of oleanolic acid in 12 species of plants are determinated by hplc, but among the different plants and botanical organs their contents are different, and the highest content in flowers

    本文對川鄂產獐牙菜屬藥用植物資源進行了野外調查、標本採集和鑒定，對12種乾燥藥材的性狀進行了描述，提供了可以鑒別的特徵。通過掃描電子顯微鏡觀察的12種本屬藥用植物的花粉粒均為單粒花粉，萌發孔有3孔型， 3溝型和3孔溝型三種類型；表面紋飾包括網狀紋，條狀?網狀紋，條狀紋和瘤狀紋四種類型。用hpias ? 1000高清晰度彩色病理圖文分析系統對葉片上表皮細胞作圖像分析，並進行方法學研究，結果表明，同種植物莖上第3節葉片中段主脈和第1側脈之間葉上表皮細胞垂周壁彎曲程度sfc值和細胞縱、橫向直徑的比值slf值，種間有顯著差異，每種都有相對恆定范圍值。
  4. The donkey - adapted strain of eiav ( dv116 ), parental strain of eiav - dla, is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s. the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period. in this study, we constructed a full - length provirus dna clone of dv116 by pcr

    本實驗中將eiav驢強毒dv體外感染驢白細胞培養物，一定時間后收獲病毒（本文中簡稱dv116 ） ，提取eiav前病毒dna ，以pcr法分別擴增並克隆了包含全長基因的三段前病毒dna片段，以雙脫氧法測定了dv116病毒全基因序列共8236個核苷酸。
  5. Except for 1. 0 kb hybridization band, other different hybridization bands were also detected which demonstrated that lea3 gene integrated into strawberry genome in different loci or copies

    Okb雜交帶外，還有其他片段人小不等的雜交帶。不同的株系形成不同的雜交譜，表明這些轉化植株來自不同的轉化細胞。
  6. Cloning and sequencing of human cbfa1 cdna fragment from osteosarcoma cell

    胞外區片段的克隆和序列分析
  7. Meanwhile, because the fragment of ctla4 extracellular domain is only 400bp, its expression will be more stable than that of ctlaig fusion protein ( 1. 2kb )

    同時，由於ctla4胞外區片段大小僅有400hp ，較ctla4ig融合蛋白zkb ）在表達菌中的表達將更為穩定。
  8. Based on the " self - migrating and surface - segregation " behavior, the amphiphile segregation surface was therefore constructed on hydrophobic pdl - la matrix, which containing the structure of peo spacer combining cell adhesion ligands to mimic the extracellular matrix ( ecm )

    基於兩親共聚物在疏水高聚物材料內的自遷移和表面富集行為，在聚乳酸材料上構建了以peo橋聯氨基酸或整合素配體多肽片段的類細胞外基質表面。
  9. 1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers

    將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。
  10. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  11. Ts87 gene fragment was picked out by screening adult t. s cdna library using sera of rabbit raised against soluble antigen of t. s and using sera of rabbit infected artificially with t. s. the ts87 fragment was ligated to vector pcdnas. l, which contains a cmv promoter / enhancer

    本研究採用通過免疫篩庫得到的旋毛蟲抗原基因片段ts87與載體pcdna3 . 1連接。該載體含有人類巨細胞病毒（ cmv ）的高效啟動子和增強子，是一種外源基因在哺乳動物細胞內高效表達的理想載體。
  12. Methods : 1. expression and purification of recombinant human ctla4 extracellular domain : a 400bp dna fragment of ctla4 extracellular domain was obtained from the pctla4 / ig plasmid with genetical technique. then, this dna fragment was inserted into ppic9 plasmid to construct the single copy plasmid of yeast expression system ( ppic9 - ctla4125 ). furthermore, the multi - copy plasmid ( ppic9k - ctla4125 ) was constructed

    重組人ctla4胞外區蛋白在酵母gs115中的表達和純化：首先通過基因操作技術，從pctla4八g質粒中擴增400hp的ctla4胞外區片段；將ctla4胞外區片段插人ppicg質粒中獲取單拷貝酵母表達質粒ppicg ctla4125並進一步構建多拷貝酵母表達質粒ppicgk （ 。
  13. Ctla4ig fusion protein was expressed by genetically fusing the dna fragment encoding 1 - 125 amino acid residues of ctla4 extracellular domain with dna fragment encoding an igg fc region. its in functional region is the extracellular domain of ctla4

    Ctla4ig融合蛋白是通過基因重組技術將編碼ctla4胞外區1 - 125個氨基酸序列的dna片段與編碼iggfc段的dna片段融合后表達生成，其功能區是ctla4胞外區。
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