胞體染色性 的英文怎麼說
中文拼音 [bāotǐrǎnshǎixìng]
胞體染色性
英文
somatobchrome- 胞 : Ⅰ名詞1 (胞衣) afterbirth2 (同一個國家或民族的人) fellow countryman; compatriot Ⅱ形容詞(同胞...
- 體 : 體構詞成分。
- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 色 : 色名詞[口語] (顏色) colour
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
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However, the advance of intracellular labeling techniques enables us not only to visualize more complete dendritic arbor for qualitative analysis, but also to examine the relation between changes in the dendritic arborization and the evoked fast postsynaptic curents - 3 - ( fpscs ) in the same neurons during the postnatal development the aim of this study was to systematically examine the postnatal changes in the configuration of fpscs evoked by the focal stimulation of the stratum radiatum of the ca1 region, and the relationship between the dendritic arborization and evoked fpscs in the rat hippocampal ca1 pyramidal neurons using whole - cell blind patch recording technique combined with biocytin intracellular labeling during the postnatal development ( postnatal day 2 - 70, p2 - p70 )
但是,細胞內染色技術的進步使我們不僅能觀察到更完整的樹突分支來用於定性研究,而且也可以在同一神經元上研究在發育過程中樹突分支的變化與誘發的快突觸后電流( fastpostsynapticcurrents , fpscs )之間的關系。因此,本研究應用盲法腦片膜片鉗記錄並結合biocytin細胞內染色方法,對發育過程中(生后2 70天)局部刺激大鼠海馬ca1區輻射層在錐體神經元誘發的fpscs的成分變化,以及ca1錐體神經元的樹突分支與誘發的fpscs的關系進行了較為系統的研究。Trial 2, effect of supplemental copper of different type on nutrition and specific immunity of mice - ii the grouping of trialt animal was the same as trial 1, at the first day, second day, third day, one mouse was injected with pha brine fluid for 10mg / kg avoirdupois after weighing in the same time in each repeat, following the 7d, 14d, 21d, 8d feeding period, after weighing, blood was made, wrigh - giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in liver and spleen
試驗二,不同形式銅對小鼠營養與特異性免疫功能的作用-試驗動物分組同試驗一,進入正式試驗期后,在每周第1天、 2天、 3天同一時間每重復選取1隻小鼠,稱重后每天按10mg kg體重肌肉注射一次植物血凝素生理鹽水溶液,並於試驗第7天、 14天、 21天、 28天稱重后尾尖取血,姬姆薩-瑞氏染色,計算t淋巴細胞轉化率,計算免疫器官指數,測定肝臟、脾臟銅含量。As the cyclin dependent kinase, cdc2 may act at multiple levels during mitosis to repress ribosome biogenesis, which lead the biosynthesis to a relative silent phase, when the most of cell ' s energy expenditure is used in chromosome condensation, breakdown of the nuclear envelope, and formation of the mitotic spindle
作為cyclin依賴激酶, cdc2的激酶活性在很大程度上抑制了細胞的合成代謝,使細胞的蛋白合成進入了一個相對的靜止期,而細胞主要的能量則被應用於細胞分裂時的染色體的濃縮、核膜的降解、紡錘體的形成等。In particular, we will focus on the ability of cell fusion that is caused by viruses to induce chromosomal instability, a common affliction of cancer cells that has been thought to underlie the malignant properties of cancerous tumours
特別是,我們將聚焦于細胞的融合能力,該能力由病毒引起來誘導染色體不穩定性,而不穩定性則是癌癥細胞中常見的異常,其被認為癌癥惡性特徵的基礎。The primary cell propagated 8 times and were small fusiform or triangle and smaller than hepatocytes with larger nucleus and less plasma ; it was 88. 2 % in the growing period of 4 ( superscript th ) generation cells and the stains of alb and ck19 were positive in immunocytochemical
所獲得的原代細胞共傳8代,細胞呈小梭形或三角形,體積較小,胞核較大,而胞漿較少;第4代細胞中進入生長期的細胞約佔88 . 2 % ;免疫細胞化學染色顯示細胞胞質中alb 、 ck19染色陽性。Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。Examination of chromsome number of the hybrids ri showed the sum of both parent chromsome besides aneuploid. the hydrid cell line ri was characterized by isoenzyme analyses, sds - page, rapd and opine synthetase assay
雜種r _ 1細胞的染色體數檢查、冠癭堿檢測、同工酶分析,可溶性蛋白sds - page電泳和rapd分析結果,都證明了其雜種特性。( 2 ) the mortality of cp cell in 10 were increasing directly related with the time in the low temperature. after 120d some cp cells died in the way of apoptosis. most nucleus of cells were condensed, and chromosome was marginated beside the nucleus inner membrane, cell sizes were reduced, dna ladder showed in dna gel electrophoresis
( 2 ) cp細胞系在10低溫下細胞死亡率與時間成正比, 120d的細胞具典型的凋亡現象,即細胞核固縮、染色體邊集在核膜內側;細胞體積變小;瓊脂糖凝膠電泳上顯示特徵性的「梯狀」帶。Over the induction process cell body became increasing spherical and retractile, exhibiting a typical neuronal perikaryal appearance. western blot a - nalysis indicated that cells exhibited increased expression of the neuronal marker nse after 5h of bfgf, atra treatment
免疫細胞化學顯示在加人bme5小時後部分細胞胞體收縮變圓,胞質呈nse染色強陽性,細胞呈有較長突起的雙極,多極型。Fos + / th + / gfap + and fos + / vp + / gfap + triple labeled n - asc could be found in the mvz, pvn and son respectively ; ( 2 ) under electronic microscope, the astrocytic processes connected closely with the dendrites or axons of the neurons, where the bilateral membranes became thick. we call transiently it electron - dense areas ( edas ). the number of edas increased remarkably following hyperosmotic stimulation ; ( 3 ) when trace retrogradely, wga - hrp was microinjected into the unilateral son, pvn or nucleus of solitary tract ( nts ) respectively using the stereotaxic method, the n - ascs formed by the neurons triple - labeled with hrp / fos / th ( or vp ) and astrocytes labeled with gfap could be found in the mvz, son and pvn respectively ; ( 4 ) after being treated with heperosmotic nacl solution, intracellular calcium concentration in cultured hypothamic neurons and astrocytes increased and then decreased
腦內gfap陽性結構也明顯增多,其分佈與fos陽性細胞分佈基本一致,表現為胞體肥大、突起粗長; ast緊密包繞在神經元周圍形成神經元- ast復合體( n - asc ) ;在mvz 、 pvn和son三重免疫組化染色切片上可見到fos + th + gfap +第四軍醫大學博士學位論文和fos vp gfap三重標記asc ; ( 2 )免疫電鏡下son內星型膠質細胞突起與神經元樹突或軸突之間接觸部位出現增厚的膜結構一電于緻密區( edas ) ,高滲刺激后數量明顯增多: ( 3 )將們個mp注入大鼠一側n卜、卜卜或孤束核( ws ) ,分別在延髓內臟帶( mvz ) 、 so和pvn內出現fos hrp th 、 fos hrp八p三重標記神經元和gfap陽性標記ast形成的n asc ; ( 4 )高滲刺激使培養神經元和ast內鈣水平先升高后降低,最後維持在比高滲刺激前稍高的靜息鈣水平上。The aglycone, steviol, exhibited greater acute toxicity than stevioside in hamsters but not in rats. steviol was clearly genotoxic after metabolic activation, inducing forward mutations in bacteria and gene mutations and chromosomal aberrations in lung fibroblasts of chinese hamsters
甜菊醇經過代謝活化后,基因毒性明顯,可引致細菌產生正向突變,以及使中國倉鼠的肺纖維原細胞產生基因突變和染色體變異。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody
選其中一株3 - h _ ( 11 )做雜交-雜交瘤細胞染色體計數,同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數:制備腹水型和上清型抗體,用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體,即紅細胞固相吸附法測雙特異中文摘要性抗體,紅細胞凝集試驗測單特異性抗人a型紅細胞抗體,間接elisa法測抗p24抗體;用腹水型抗體做耐熱性及耐凍融實驗。Dosage compensation in female mammals is achieved by inactivating the transcription of one of the x chromosome
雌性動物的劑量補償效應是通過細胞中一條x染色體的轉錄失活來實現的。Nevoid basal cell carcinoma syndrome, also known as gorlin syndrome, is an autosomal dominant inherited disorder which is characterized by the presence of multiple maxillary keratocysts, facial basal cell carcinomas and kinds of musculo - skeletal disturbances
摘要痣樣基底細胞癌綜合征是一種罕見的常染色體顯性遺傳疾病,以頜骨多發性角化囊腫,皮膚痣樣基底細胞癌及多種骨骼異常為主要臨床表現。Along with the development of cellular genetics and tumor etiology, a lot of attention has been paid to the relation between chromosome instability and tumor formation
摘要隨著細胞遺傳學與腫瘤病因學研究的不斷深入,染色體不穩定性與腫瘤形成的關系越來越受到人們的重視。Under mirror obviously ; the cell apoptosis divides into the early stage, the intermediate stage, stage of formation of apoptosis body and the later period stage ( three issuses of four stges ), its ultrastructure is mainly observed the cellular form, the cytoblastema, the nucleus, the chromatin change, the special sign of cell apoptosis - apoptosis body
鏡下可見:細胞凋亡分為早期階段、中期階段、凋亡小體形成階段和晚期階段( 3期4階段) ,其超徽結構主要可見到細胞形態、胞漿、胞核、染色質的變化,及細胞凋亡的特異性標志凋亡小體。At the beginning, the cytoplasm is evently distributed, then it is polar - distribution and at last it only remains between the apical cap and the sperm nucleus. during the period of spermatogonium, chromatin in the nucleus is in the shape of conglomeration and the nucleus is big ( i. e. the proportion of the nucleus to cytoplasm is bigger than spermatocyte ). at the stage of primary spermatocyte, chromatin accumulates to sc complexity. the nucleus of the mature sperm takes the shape of a plate and filmentous chromatins exist freely in uncondensed nucleus
精子發生過程中,細胞器由少到多,到最後解體;細胞質從均勻分佈,到精細胞期極性分佈,最後殘留于成熟精子的頂帽和精核之間;細胞核在精原細胞期,染色質異固縮,胞核大,即核質比大於后續的精母細胞;在初級精母細胞中,染色質凝聚為sc復合體,到成熟精子,精核呈碟狀,染色質呈絲狀游離于非濃縮的核中。Comparing with controls, the amount and dyeing of immunopositive cells of il - 1 receptor type i in rat paraventricular nucleus of the hypothalamus and the supraoptic nucleus were significantly enhanced by intraperitoneally lps or seb injection ( po. 05 ), and the dyeing areas and processes of positive cells were enlarged
與對照組比較, lps或seb腹腔注射后大鼠下丘腦室旁核和視上核中表達型il - 1受體的細胞數量顯著增加,染色加深( p 0 . 05 ) 。陽性細胞的染色面積增大,突起增長。Cells were collected after being passaged 5 times and used to identify infection by rt - pcr, direct fluorescent antibody, sandwich elisa. results indicated that integrated particles of csfv could be obtained from constructed full - length cdna
連續傳代5代以後,收集各w摘要代細胞至第10代,採用rt一pcr 、直接熒光抗體染色和夾心elisa技術進行鑒定,結果均為陽性。分享友人