胰蛋白原 的英文怎麼說

中文拼音 [yídànbáiyuán]
胰蛋白原 英文
protrypsin
  • 胰 : 名詞[生理學] （胰腺） pancreas
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. The results showed that : adding tryptone, soy peptone. beef extract, com extract and cys - hcl to jaj could obviously promote the growth of blm and bbm ; by the orthogonal experiment of three elements and three levels, a satisfying jaj compound medium was acquired which included corn extract ( 0. 3 % ), soy peptone ( 0. 05 % ) and cys - hcl ( 0. 025 % ). nextly, after establishing a selective bifidobacterium medium, the effects of jaj on the growth of bifidobacteria in vivo were studied, using healthy mouse of kunming species as experimental animal

    研究了以菊芋汁為主要原料的雙歧桿菌培養基，大量試驗結果表明，在菊芋汁中添加胰蛋白腖、牛肉膏、大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽等成分，對雙歧桿菌有明顯的促進生長作用；利用大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽設計了三因素三水平的正交試驗，確定了菊芋汁復合培養基的優化配方：菊芋汁+ 0 . 3玉米漿+ 0 . 05大豆蛋白腖+ 0 . 025半胱氨酸鹽酸鹽。
  2. 2. morphologic studies of hepatocytes in different nutritive elements medium. objective : this study was designed to determine if different nutritive elements, such as transferrin, insulin, nicotinamide, p - mercaptoethanol ( - me ) and hepatocytes growth factor ( hgf ) affected the morphology of the primary hepatocytes in short term culture

    目的：本研究在普通培養液中添加轉鐵蛋白、牛胰島素、煙酷胺、 p琉基乙醇和促肝細胞生長因子，觀察這些營養成分對原代短期培養的小鼠肝細胞的形態學的影響，旨在探討一種適用於原代肝細胞的培養方案。
  3. Preparation and application of monclonal antibodies against trypsinogen activation peptide

    抗胰蛋白酶原激活肽單克隆抗體的制備及應用
  4. In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method

    第一部分，我們按照交替氧化酶（ alternativeoxidase ， aox ）位於線粒體內膜外側親水區s螺旋的氨基酸序列，用固相法合成由12個氨基酸殘基組成的多肽，將此多肽與-牛胰凝乳蛋白酶原a相連，用作半抗原，免疫家兔制備人工合成12肽的抗體。
  5. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  6. The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells

    組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。
  7. Hyperinsulinemia in elderly primary hypertension patients complicated by microalbuminuria

    老年原發性高血壓合併微量白蛋白尿患者的高胰島素血癥
  8. Prolonged the culture term, hepatocytes both of experimental groups and control groups became pyknotic and were detached from the wall or fibroblasts became prominent in the culture pl ate. it was important to note that none of the condition caused an increase in the number of cell in the whole experimental process. conclusion : the primary hepatocytes in medium of additional special nutritive may benefit in comparison with common medium

    結論：在培養液中添加轉鐵蛋白、牛胰島素、煙酚胺、 p琉基乙醇以及促肝細胞生長因子，可以促進原代小鼠肝細胞的貼壁生長，並且可以改善肝細胞在體外的代謝，使之維持良好的生活狀態並且延長體外生存時間。
  9. When the hameoly - mph were pretreated with activators such as sds, trypsin and zymosan, their po acti - vities increased significantly, reaching about twice of the untreated. this indicated that the po activities exist in the hameolymoh of penaeus chinensis and penaeus

    結果表明，中國對蝦和南美白對蝦血清中都存在po ，主要以酚氧化酶原（ propo ）的形式存在，並且都可以被胰蛋白酶、 sds和酵母聚糖激活。
  10. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。
  11. In this study, one strain of avian rotavirus was isolated from a chicken with natural diarrthoea. isolation and cultivation of the rotavirus was attemped by the inoculating on monolayers of marc 145 cells with a suspension of faecal material containing the rotavirus

    本文對發生在山東某地雞流行性腹瀉病例的病原進行了研究。用胰蛋白酶處理病雞的糞便和腸內容物，接種marc145細胞，盲傳數代後分離到一株病毒。
  12. In he - 3 and de - 3, 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously. in he - 2 and de - 2, 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously, some of these different proteins are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction. it shown that gene expression have significant difference between diploid and haploid during embryos developing

    分別選取其中70個分辨好的差異蛋白質點進行原位胰蛋白酶酶解，酶解的肽片段用基質輔助激光解析電離分行時間質譜（ maldi - tofms ）得到肽質指紋圖譜，再在網上peptident軟體中搜索swiss - prot和trembl數據庫，初步鑒定了he - 3和de - 3上32個差異蛋白質點，其中包括16個表達量上有差異的蛋白質點（ p 0 . 05 ）和16個有無表達的差異蛋白質點，並且初步鑒定了he - 2和de - 2上25個差異蛋白質點，其中包括5個表達量上有差異的蛋白質點（ p 0 . 05 ）和20個有無表達的差異蛋白質點。
  13. Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )

    Pbv220 ? hng在大腸桿菌中未檢測到表達，后兩個克隆在大腸桿菌bl21 （ de3 ）中獲得高效表達， hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右；經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體（ igg ）發生抗原抗體結合反應。
  14. After sds - page and densitometric scan analysis, the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 %. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %

    今士考個二目卜乙成功構建了ptxbi一hng及ptxbi一m一insulin原核表達克隆，並獲得了高效表達，經過純化得到純度人於80 %的融合蛋白，並對人胰島素突變體融合蛋白進行了初步活性測定。
  15. 2. the primary culture of silkworm embryonic cells ( 1 ) this dissertation took silkworm blastokinesis embryo as the materials of primary culture and found a better method of fragment embryo to primary culture by comparing the methods of fragment embryo that mincing embryo by scissors and digest embryo by trypsin after mincinging. that is the latter

    2家蠶胚胎細胞的原代培養門）本研究以家蠶反轉期胚胎為材料，採用組織塊法和胰蛋白酶半消化法進行家蠶胚胎細胞的原代培養，建立了適應反轉期胚胎細胞原代培養的方法，即胰蛋白酶半消化法。
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