胰蛋白酶化 的英文怎麼說

中文拼音 [yídànbáihuà]
胰蛋白酶化 英文
trypsinize
  • 胰 : 名詞[生理學] （胰腺） pancreas
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. The he was strongly inhibited by sbti and apmsf, and very sensitive to pmsf, lbti, and tlck, while not sensitive to chymostatin, bestatin, leupeptin, tpck, pepstatin, nem and iam. all these results imply that brine shrimp he was most probably a trypsin - type serine protease. the he could be strongly inhibited by edta in a dose - dependent manner, and 50 mmol / l edta exhibited more than 56. 5 % inhibition

    對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。
  2. Compared to control, retention times of digesta in whole alimentary tract of immunized animals inc reased by 20 hours ( to use cumlative excretion of 5 % marker as reference ). immunoneutralization of ss significantly augmented activities of digestive enzymes ( proteolytic, trypsin, chymotrypsin, amylase ) in pancreas and the small intestine ( control and immunized animals were 1693. 67unit / g, cp, 2728. 33 unit / g, cp, 3055. 50 unit / g, cp, 12. 9x106 unit / g, cp ; 2. 57x 102unit / g, cp, 1. 20x103unit / g, cp, 1. 12x 103unit / g, cp, 2. 98x 107unit / g, cp ft 2451. 33 unit / g, cp, 2904. 17 unit / g, cp, 4279. 33 unit / g, cp, 20. 61 x 106 unit / g, cp ; 6. 45 x 102unit / g, cp, 2. 53 x 103unit / g, cp, 1 - 83 x 103unit / g, cp, 5. 77 x 107unit / g, cp, respectively, p < 0. 05 or p < 0. 01 )

    12ng ml ， 0人su vg ，各指標比較均差異不顯著， p 0刀5人兔疫組動物的食糜消化道滯留時間明顯增加（以指示劑累計排出50為標準，兔疫組較對照組大約增加20小時） ，與此同時， ss免疫中和也提高了胰腺和消化道各種消化酶的比活力（對照組和免疫組胰腺，小腸食糜總蛋白酶，胰蛋白酶， 」糜蛋白酶和澱粉酶比活分別為1693石7unit g ， cp ， 2728
  3. Studies on resistant induction of citral to candida albicans in vitro

    永川豆豉胰蛋白酶抑制劑的分離純化及其降糖活性研究
  4. Fixable conditions and function of trypsin

    胰蛋白酶固定化條件及穩定性對比研究
  5. In this paper, the relationship between cyanide - resistant respiration and expression of alternative oxidase ( aox ) using polyantibodies prepared by synthetic polypeptide in mung bean seedling, as well as the effects of vernalization on cyanide - resistant respiration and expression of aox in winter wheat were studied respectively. in the first part, twelve peptides, including eight conservative amino acid residues in the amino acid sequence of hydrophilic s helix of aox. were synthesized by solid - phase method

    第一部分，我們按照交替氧化酶（ alternativeoxidase ， aox ）位於線粒體內膜外側親水區s螺旋的氨基酸序列，用固相法合成由12個氨基酸殘基組成的多肽，將此多肽與-牛胰凝乳蛋白酶原a相連，用作半抗原，免疫家兔制備人工合成12肽的抗體。
  6. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  7. The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells

    組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。
  8. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  9. In artificial trypsin digestive system with ph7. 4, with the increasing content of trypsin and prolonging of digesting time, the entire pigg decreased gradually

    在ph7 . 4的人工腸液中，隨著胰蛋白酶加量的增加和消化時間的延長，完整pigg的存留率也逐漸減少。
  10. When the hameoly - mph were pretreated with activators such as sds, trypsin and zymosan, their po acti - vities increased significantly, reaching about twice of the untreated. this indicated that the po activities exist in the hameolymoh of penaeus chinensis and penaeus

    結果表明，中國對蝦和南美白對蝦血清中都存在po ，主要以酚氧化酶原（ propo ）的形式存在，並且都可以被胰蛋白酶、 sds和酵母聚糖激活。
  11. The results indicate that because of the improvement in two aspects mentioned above, the successful probability of the docking prediction is increased from unbound structures. for the case of enzyme trypsin - inhibitor appi, although the large conformational change occurs to the arg 15 side chain of appi upon complex formation, the native - like structure was still found and ranked first

    其中，在胰蛋白酶appi復合物結構預測中，盡管抑制劑appi的15號殘基arg側鏈在復合物形成過程中發生了較大的構象變化，但其近天然結構仍然被找到了，並在打分中排在了第一位。
  12. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。
  13. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  14. In this experiment, the method of trypsin digestion was improved, and that several kinds of mouse fibroblasts and porcine fibroblasts were successfully dissociated and cultured

    實驗中改進了胰蛋白酶消化法，成功地分離和培養了多種鼠成纖維細胞和多種豬成纖維細胞。
  15. Then, the sensitivity of the subunits to trypsin traetment was compared by polypeptide pattern among these five psii preparations with sds - page

    分別用不同濃度的胰蛋白酶對這5種ps制劑進行限制性蛋白酶解處理， sds - page分析多肽組分變化。
  16. 2 ) hanks " buffer of trypsin not being added edta could obtain better the growth potential and the subsequent attachment of harvested skin cells t then it being added edta have better digested potent

    與添加edta相比，用只含胰蛋白酶的hanks '液處理貼壁細胞，所回收的傳代細胞存活能力較好；而添加edta的胰蛋白酶消化液消化能力較強。
  17. 3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin

    對于秦川牛皮膚上皮細胞與成纖維細胞的純化： dispase冷消化單細胞培養的效果最佳，可獲得純化的上皮細胞系，而根據二者對胰蛋白酶的敏感性不同的分離方法、反復貼壁法適宜於獲得純化的成纖維細胞系。
  18. Both xyna and xynb did n ' t display cellulase activity both xyna and xynb were treated by pepsin or trypsin for 30 min under 37c, their activity were n ' t decreased

    胃蛋白酶、胰蛋白酶37下處理30分鐘，對酶活性無影響。將純化出的xyna及xynb進行n端序列測定。
  19. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。
  20. 2. the primary culture of silkworm embryonic cells ( 1 ) this dissertation took silkworm blastokinesis embryo as the materials of primary culture and found a better method of fragment embryo to primary culture by comparing the methods of fragment embryo that mincing embryo by scissors and digest embryo by trypsin after mincinging. that is the latter

    2家蠶胚胎細胞的原代培養門）本研究以家蠶反轉期胚胎為材料，採用組織塊法和胰蛋白酶半消化法進行家蠶胚胎細胞的原代培養，建立了適應反轉期胚胎細胞原代培養的方法，即胰蛋白酶半消化法。
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