脂粒體 的英文怎麼說
中文拼音 [zhīlìtǐ]
脂粒體
英文
lipochondria-
We found nuclear deformation lymphocytes with increased heterochromatin and impaired thymus epithelium cells with increased lysosomes and deformation of mitochondrias. ( 4 ) effects on mouse liver of so2 challenge : so2 can cause significant liver injury. he staining showed several kinds of necrosis of liver including spot necrosis, focal necrosis and submassive necrosis infiltrated with lymphocytes, monocytes, few neutrophils and eosinophils ; tem observation showed fatty degeneration with dispersion of fatty droplets and dilation of rough endoplasmic reticulums, acid degeneration with significant hyperplasia of mitochondrias, necrosis of hepatocytes with karyorrhexis and other organelles losing their normal structure
( 4 )二氧化硫染毒對小鼠肝臟的組織學結構有明顯影響,可引起肝臟點狀壞死、灶狀壞死甚至片狀壞死,伴隨不同程度的炎性細胞浸潤;透射電鏡觀察發現二氧化硫可引起肝細胞脂肪變性、嗜酸性顆粒變和壞死,脂肪變肝細胞中可見大小不等的脂滴存在,嗜酸性顆粒變肝細胞中可見線粒體明顯增生,壞死肝細胞可見細胞核結構破壞,細胞器減少,細胞膜不完整。Mitochondria convert the chemical energy of carbohydrates and fats into energy forms which the rest of the cell can use when needed.
線粒體可將脂肪與碳水化合物的化學能轉化為細胞的其餘部分需要時可以利用的能源形式。Extensive mitochondrial dna polymorphisms were found among lagurus lagurus, mus musculu , rattus norvegicus and mice. the findings will help us to understand the dispersion and evolution of these animals
通過瓊脂糖凝膠電泳對這些片段進行測定,同時估算出草原兔尾鼠線粒體dna的長度約為16 . 6kb 。They act as electron - transport agents and play an important role in the electron - transport chains of respiration, photosynthesis, and the microsomal system responsible for the desaturation of fatty acids in heterotrophic bacteria
它們作為電子傳遞物在呼吸電子遷移鏈、光合作用及微粒體系統中起重要作用,它還負責異養細菌中脂肪酸的去飽和作用。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。Toughening thermoset matrix composites with interlayered thermoplastic particles is an effective method to improve fracture toughness and impact resistance without sacrificing hot / wet properties. furthermore, transverse strength, fatigue resistance and solvent resistance can also be enhanced. drapability and tackness of interleaved prepreg is maintained as conventional level
對樹脂基體復合材料採取層間顆粒增韌是提高復合材料層壓板韌性、抗沖擊能力和抗分層能力的有效途徑,在保持原有復合材料濕熱性能的前提下,通過層間顆粒增韌,還可以改善垂直纖維方向的強度、抗疲勞性能以及耐溶劑性,並不影響原有的成型工藝。Alcohol, the most common cause, is a hepatotoxin that interferes with mitochondrial and microsomal function in hepatocytes, leading to an accumulation of lipid
乙醇是最常見的病因,作為肝毒素能阻礙肝細胞內的線粒體和微粒體的功能,導致脂質沉集。The factors which effect the fsmf filler to build up strength and toughness in the resin matrix composite materials include the size and the shape of fsmf grain the glue performance on the interface of resin matrix and the grain of fsmf
同時得出fsmf顆粒與樹脂基體界面上的粘結性能、 fsmf顆粒的大小和fsmf顆粒的形狀等是影響fsmf填充樹脂復合材料增強增韌的重要因素。In order to obtain well effect on the improve the strength and toughness, the interface on the grain and the resin matrix should have a good glue performance ; the diameter of the grain should be small and the shape component of grain patch and fiber should be suitability
要獲得好的增強增韌效果,填料顆粒與樹脂基體界面上應有好的粘結性能;顆粒粒徑應小且填料粉體中粒狀、片狀和纖維狀的組分應適當。Glycerolipids are synthesized from glycerol 3 - phosphate by the addition of fatty acid chains and a head group
植物體中的線粒體、質粒以及內質網可合成甘油脂。The result of ir and the tga experiment indicate that nano - sio2 can improve the heat - resistance properties evidently ( the nano - composite nearly not decompose below 400 ). through the analysis of sem, we can conclude that nano - sio2 can increase the toughness of the composite
紅外( ir )和熱重分析( tga )研究表明,納米sio2粒子能顯著的提高樹脂基體的耐熱性能和疏水性能,改性的復合材料能在400以前無明顯的熱分解。Abstract : we have investigated the ultrastructural changes of the biopsied muscle specimens from 8 cases of metabolic myopathies including 3 cases of glycogenosis, 2 cases of lipid storage myopathy and 3 cases of mitochondria myopathy. diagnosis and differential diagnosis with tem for metabolic myopathy as well as the significance of some ultrastructural changes were discussed in this study
文摘:本文對3例糖原累積病, 2例脂質沉積性肌病和3例線粒體肌病共8例代謝性肌病肌活檢標本的超微病理改變進行了觀察分析,對代謝性肌病的超微病理診斷、鑒別診斷以及某些病理改變的診斷意義進行了初步探討。Relationship between sperm plasma membrane translocation of phosphatidylserine and mitochondrial transmembrane potential
精子質膜磷脂酰絲氨酸外翻與線粒體膜電位的關系2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞,進行瞬時表達, 48小時后,用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鑒定、純化重組質粒后,脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞( cho - dhfr ~ - )中, g418選擇轉染子並克隆化培養,經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄,獲得4株穩定表達54位密碼突變型mbl的cho細胞。In the resin matrix composite materials filled with fsmf, the load is undertaken by the grain of fsmf and the resin matrix. the grain of fsmf restricts the deformation of the resin matrix, so the strength is improved. furthermore, the grain of fsmf can consume the energy of strain and controls the crack expand, so build up toughness
在fsmf填充樹脂復合材料中,載荷是由fsmf顆粒和樹脂基體共同承擔的, fsmf顆粒以機械約束方式限制樹脂基體變形,從而產生強化; fsmf顆粒可以引發銀紋和剪切帶等損傷消耗應變能,並通過鈍化和釘扎機制阻礙裂紋的擴展,從而產生增韌。In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因,克隆到桿狀病毒表達載體pfastbacl中,構建成重組轉座載體pfb1 - cee ,轉化dh10bac大腸桿菌感受態細胞,篩選陽性菌落,抽提大分子質粒dna ,獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ,脂質體介導轉染sf9昆蟲細胞,出現細胞病變后,收集含有重組桿狀病毒顆粒的培養上消,重新感染sf9細胞,收集sf9細胞,進行12 . 5 sds -聚丙烯酰胺凝膠電泳,可見表達的蛋白條帶。Positive single clonal cells were selected with g418 and pcr. after proliferating, the transfected cells immobilized and cultured in soldium alginate were induced by hormone
利用脂質體包裹重組質粒plncxhlf ,導入到小鼠乳腺癌細胞株ma - 782中, g418及pcr篩選獲得陽性單克隆細胞。Objectives firstly to prepare the vaccine of human dcs and tumor cells transfectde with pcdna3 - cea, and to observe the induction of carcinoembryonic antigen ( cea ) - specific cytotoxic t - lymphocyte responses in vitro
用脂質體將cea表達質粒轉染人dc ,觀察其對cea表達陽性的腫瘤的特異性免疫作用。分享友人