脂質粒 的英文怎麼說

中文拼音 [zhīzhílì]
脂質粒 英文
lipid granule
  • 脂 : 名詞1. （動植物所含的油質） fat; grease; tallow 2. （胭脂） rouge 3. （姓氏） a surname
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 脂質 : fatty substance
  1. Particles deposited in the alveoli are immediately subjected to coating by surface active lipid materials.

    當粒子沉澱到肺泡中時，立刻會遭到表面活性的脂質物質的覆蓋。
  2. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  3. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  4. To find dnaase in the earthworm the tissue extract of earthworm with different concentration reacted with rna, circular dna, linear dna for an hour at 37, then the producetion was detected by 1 % agrose gel. the tissue extract of earthworm, the tissue protein extract of earthworm and the tissue extract of earthworm without protein reacted with pbv220 - r - inf for an hour at 37, then the producetion was detected by 1 % agrose gel. 2

    雙胸蚓組織中dna酶的發現用不同濃度的雙胸蚓組織提取液與rna 、環狀dna及線狀dna在37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察；雙胸蚓組織粗提取液、雙胸蚓組織蛋白粗提取液及雙胸蚓組織去蛋白提取液分別與pbv220 - ? inf質粒37反應1小時後用1的瓊脂糖凝膠電泳對其反應產物進行觀察。
  5. Determination of impurities particles number in polyvinyl chloride paste resins

    聚氯乙烯糊樹脂中雜質粒子數測定方法
  6. Alcohol, the most common cause, is a hepatotoxin that interferes with mitochondrial and microsomal function in hepatocytes, leading to an accumulation of lipid

    乙醇是最常見的病因，作為肝毒素能阻礙肝細胞內的線粒體和微粒體的功能，導致脂質沉集。
  7. Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta. after sonication, debris was removed by centrifugation at 10000 x g for 15 min at 4

    挑取轉化后的大腸桿菌提取質粒， ecori和hindln酶切質粒進行鑒定，瓊脂搪電泳顯示含有大約800kb的目的片段。
  8. Glycerolipids are synthesized from glycerol 3 - phosphate by the addition of fatty acid chains and a head group

    植物體中的線粒體、質粒以及內質網可合成甘油脂。
  9. Mutated plasmid was transformed into e. coli tg1 cells to produce engineered peptide, then the peptide was purified by cm sepharose ion - exchange column. in vitro bactericidal assay and drug withdrawal were used to identify the bioactivity of the engineered peptide. the planar lipid bilayer membrane was used to assay the electrophysiology of the engineered peptide. toxicity studies on mammalian cells were used to assay the toxicity of the engineered peptide

    將重組質粒轉化入大腸桿菌tgi工程菌中，生產構建的工程多膚，離子交換純化后獲得工程多膚初步純化產物，體外抗菌試驗、藥物撤離試驗檢測工程多膚的抗菌活性，在人工脂質膜上測定其形成離子通道的特性以初步研究抗菌機理， ?並觀察其對真核細胞的毒性作用。
  10. Abstract : we have investigated the ultrastructural changes of the biopsied muscle specimens from 8 cases of metabolic myopathies including 3 cases of glycogenosis, 2 cases of lipid storage myopathy and 3 cases of mitochondria myopathy. diagnosis and differential diagnosis with tem for metabolic myopathy as well as the significance of some ultrastructural changes were discussed in this study

    文摘:本文對3例糖原累積病， 2例脂質沉積性肌病和3例線粒體肌病共8例代謝性肌病肌活檢標本的超微病理改變進行了觀察分析，對代謝性肌病的超微病理診斷、鑒別診斷以及某些病理改變的診斷意義進行了初步探討。
  11. 2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively

    3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞，進行瞬時表達， 48小時后，用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。
  12. The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level

    抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。
  13. Experimental study of sanshanxiao granules on the levels of insulin and lipid peroxidation in model rats of diabetes

    三山消顆粒對糖尿病大鼠胰島素水平及脂質過氧化反應的影響
  14. The cytoplasmic clearing is due to glycogen content which can be demonstrated by pas positiity

    瘤細胞較大，多邊形或圓形胞界清晰，胞漿透亮含糖原、脂質或紅染顆粒， pas染色陽性反應。
  15. The main mechanisms of earthworms ' resistance to metal pollutants are also elaborated : ( 1 ) its lipid antioxidative enzyme system helps relieve the stress of oxidation ; ( 2 ) compartment and immobilization of metals ; ( 3 ) process of chelating and detoxicification ; ( 4 ) lysosome and cellular plasmid are activated to restrain activity of heavy metals

    並闡述了蚯蚓對重金屬的主要耐性機制： （ 1 ）脂質過氧化保護酶系統緩解氧化脅迫； （ 2 ）分隔、固定作用； （ 3 ）螯合解毒作用； （ 4 ）溶酶體和細胞質粒抑制重金屬活性。
  16. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  17. Transparency is unusual because cells have organelles ? internal structures such as the nucleus ( which stores dna ), the energy - producing mitochondria, and the golgi apparatus and endoplasmic reticulum, which are important in the synthesis of proteins and lipids

    透明並不容易達成，因為細胞中有稱為胞器的內部構造，例如儲存dna的細胞核、產生能量的粒線體，以及對合成蛋白質與脂質很重要的高爾基體與內質網。
  18. The effect of shenyanling granule for the lipid metabolism in patients with chronic idiopathic glomerulonephritis

    腎炎靈顆粒劑對慢性原發性腎小球疾病脂質代謝的影響
  19. Solid lipid nanoparticle is a new drug delivery system of nano - range

    摘要固體脂質納米粒是一種新型的納米給藥系統。
  20. Positive single clonal cells were selected with g418 and pcr. after proliferating, the transfected cells immobilized and cultured in soldium alginate were induced by hormone

    利用脂質體包裹重組質粒plncxhlf ，導入到小鼠乳腺癌細胞株ma - 782中， g418及pcr篩選獲得陽性單克隆細胞。
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