脲基乙內酰 的英文怎麼說
中文拼音 [jīyǐnèi]
脲基乙內酰
英文
ureidohydantoin-
Mass spectrometry technique will play more and more role in the field of sequence analysis. standard amino acid thiohydantoins are required as reference standard for development of c - terminal protein sequencing based on the thiohydantoin procedure
在蛋白質c端（異）硫氰酸法順序測定技術中，標準氨基酸乙內酰硫脲（ th - aa ）的制備是必須首先要解決的問題。Hydantoinases which consist of hydantoin hydrolase, n - carbamoylase and sometimes hydantoin racemase can hydrolyze d, l - 5 - substituted hydantoin into corresponding optically active amino acids. therefore, the hydantoinases from micro - organisms are widely applied to the industrial production of natural and unnatural amino acids. microbial hydantoinases are considered as industrial enzymes from the origin of life
乙內酰脲酶是廣泛存在於動物、植物和微生物的一類酶系，包括乙內酰脲水解酶（ hydantoinhydrolase ， hyuh ）和n -氨甲酰基氨基酸水解酶（ n - carbamoylase ， hyuc ） ，有些情況下還包括乙內酰脲消旋酶（ hydantoinracemase ， hyur ） 。The l - n - carbamoylase of arthrobacter bt801, coded by the hyuc gene, is the rate - limiting and the only stereoselective enzyme. hydantoin hydrolase gene ( hyuh ) and n - carbamoylase gene ( hyu c ) were amplified from the plasmid of puc18 - 169 by pcr and, at the same time, activity expression was obtained in e. coli and pichia pastor is gs115 respectively
節桿菌bt801的乙內酰脲水解酶催化乙內酰脲類化合物水解開環， n -氨甲酰基氨基酸水解酶是該菌乙內酰脲酶系中惟一具有立體選擇性的酶，也是整個反應體系的限速酶。To improve the production and activity of the enzyme, the hydantoin hydrolase gene ( hyuh ) was cloned into e. coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively. two expression plasmids were thus constructed
用pcr技術從質粒puc18 - 169中擴增出l -乙內酰脲水解酶基因（ hyuh ）和n -氨甲酰基氨基酸水解酶基因（ hyuc ） ，分別在大腸桿菌和畢赤酵母中實現了活性表達。分享友人