腸噬菌體 的英文怎麼說
中文拼音 [chángshìjūntǐ]
腸噬菌體
英文
bacteriophagum intestinale-
E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ,以輔助噬菌體m13k07超感染,進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。Water quality - detection of human enteroviruses by monolayer plaque assay
水質.用單分子層噬菌斑序列檢測人體腸病毒The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體( 100ng l )包被酶標板,加入制備好的肽庫,用tbst洗去非特異結合的噬菌體,加0 . 2mol l甘氨酸-鹽酸( ph2 . 2 ) ,室溫放置10min以洗脫特異結合的噬菌體, 2mol ltris - hcl ( ph9 . 1 )中和后,取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定,其餘噬菌體擴增後用于下一輪篩選,共重復3輪淘洗。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反轉錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因,並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因;將mg _ 7單鏈抗體基因插入pcantab5e ;將連接產物轉化感受態tg1大腸桿菌,制備細菌形式的mg _ 7重組噬菌體抗體庫;通過菌落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組噬菌體抗體庫的容量和重組率。High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon
將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上,使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。The amplified phage be used repeat the selection. the selection was repeat three times. the titter of phage indicated that the eluted phage enriched with the rounds increasing
山西醫科大學碩士學位論文第三次淘洗的噬菌體感染大腸桿菌xli一blue ,鋪制平板,隨機挑取18個分離良好的噬斑,制備噬菌體原種。The study provides an entropy assessment from an ecological perspective indicating parasite - host evolutions between bacteriophage and escherichia coli during induction
摘要本研究以生態觀點之亂度評估法來衡量噬菌體及大腸桿菌于誘導過程之宿主-寄生蟲消長現象。Using these vectors, expression of the pks gene was achieved both in streptomyces lividans and in e. coli in a heat - dependent manner, suggesting that the lambda promoter and temperature - sensitive lambda represser functioned in s. lividans as well as in e. coli
利用這些質粒在變鉛青鏈黴菌和大腸桿菌中均表達出pks蛋白,兩種宿主中的表達都是熱依賴的。暗示噬菌體啟動子和溫敏型阻遏物在變鉛青鏈黴菌和大腸桿菌中都具有功能。Examination of salmonellae, shigellae and diarrhoea causative escherichia coli by means of the diagnostic typing phage set for enterobacteriaceae
沙門氏菌志賀氏菌和致瀉大腸埃希氏菌的腸桿菌科噬菌體檢驗方法Microbiological examination of food hygiene examination of salmonellae, shigellae, and diarrhoea causative escherichia coli by means of the diagnostic typing phage set for enterobacteriaceae
食品衛生微生物學檢驗沙門氏菌志賀氏菌和致瀉大腸埃希氏菌的腸桿菌科噬菌體檢驗方法Two bifunctional streptomyces - e. coli vectors were constructed that contained the phage lambda promoter ( pr ) upstream of the his6 - tagged recombinant pks gene
構建了兩個鏈黴菌-大腸桿菌雙功能pks表達質粒,在重組pks基因上游攜帶有噬菌體啟動子。Water quality - detection of human enteroviruses by monolayer plaque assay ; german version en 14486 : 2005
水質.通過單層噬菌斑檢驗探測人體腸道病毒Meanwhile, highly active microbial agents also can enhance the phagocytosis of phagocytic cells, improve animals ' intestinal micro ecology ; improve animals ' immune response and resistance to disease
同時,高活性微生物菌劑還能夠增強吞噬細胞的吞噬能力,改善動物腸道的微生態,提高動物機體免疫應答能力,增強抗病能力。In our previous study, yggg, speb were found in 2. 8kb dna fragment that could bound era probe by using e. coli genome phage expression library to screen era binding protein
根據本課題組篩選era結合蛋白時獲得的噬菌體中的插段包含大腸桿菌的ygggspeb基因,本研究分析了era與ygggspeb的相互作用。分享友人