腸形菌素 的英文怎麼說
中文拼音 [chángxíngjūnsù]
腸形菌素
英文
coliformin-
Colicin ia belongs to the family of bactericidal proteins, the channel - forming domain of which can form lethal ion channel in the membrane of escherichia coli and related strains such as shigella sonnei
大腸菌素ia是可形成離子通道的一種細菌素，其通道結構域（ d451 ? i626 ）能在同種異株的大腸桿菌內膜上形成致死性離子通道。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Indication ： for the treatment of bacterial enteritis caused by e. coli, salmonella spp., proteus, dysentery bacillus, an d other organisms susceptible to neomycin
用於治療禽畜的大腸桿菌、沙門氏菌、變形桿菌、痢疾桿菌以及其他對新黴素敏感菌感染引起的腸炎。Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2
含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。Because expressed in escherichia coli, ricin a chain is not degraded easily for its non - glycosylation. our experiments lay a foundation for the researches that rta conjugates a monoclonal antibody to synthesize immunotoxins and the immunotoxins are used for cancer - targeted therapy
蓖麻毒素a鏈在大腸桿菌中表達，因無糖基化修飾，克服了進入體內后被迅速降解的缺點，為進一步與單鏈抗體基因嵌合表達形成單鏈免疫毒素，對腫瘤細胞進行導向治療研究奠定基礎。Because of the capability of gm1 binding, ctb can enhance vaccine efficacy by inducing mucosal and systemic immune responses. plant bioreactor is a safe and cheap protein production system
由於ctb的載體功能， ctb和cs3融合的形式（ ctb cs3 ）免疫原性優于單獨存在的ctb和cs3單個蛋白，並可達到雙抗霍亂弧菌以及毒素大腸桿菌的目的。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。Construction and expression of a new human growth hormone isoform - gst fusion protein
人新的生長激素異形體基因的克隆及其在大腸桿菌中的表達If defecate time is much, when working when defecate rare won ' t do form, can take sick at heart of pai of amoxilin, fluorine a kind of antibiotic 3 5 days, can eliminate bowel unusual bacterium is active inside the path and ferment produce gas to cause bilge gas and defecate deformed phenomenon
假如大便次數多，大便時干時稀不成形，可服用阿莫西林、氟哌酸一類抗生素3 5天，可消除腸道內異常細菌活躍而發酵產氣引起脹氣及大便不成形的現象。分享友人