腸桿菌素 的英文怎麼說
中文拼音 [chánggǎnjūnsù]
腸桿菌素
英文
colistin-
E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd
以構建的噬菌粒psurfpga轉化具有琥珀突變的大腸桿菌xl1 - blue ，以輔助噬菌體m13k07超感染，進行青霉素g酰化酶基因的表達和在噬菌體表面的展示。It appeared that in that country the disease was caused by an antibiotic-resistant strain of e. coli that was transmitted by the boar.
在那個國家，此病是由一株抵抗抗菌素的埃希氏大腸桿菌菌株引起，由公豬傳播的。Colicin ia belongs to the family of bactericidal proteins, the channel - forming domain of which can form lethal ion channel in the membrane of escherichia coli and related strains such as shigella sonnei
大腸菌素ia是可形成離子通道的一種細菌素，其通道結構域（ d451 ? i626 ）能在同種異株的大腸桿菌內膜上形成致死性離子通道。However, the receptor and translocation domains of the colicin ia can only recognize the inner and outer membrane receptors of the escherichia coli, therefore the wild type colicin ia can not be developed into antibiotics against other types of bacteria
然而由於其信號識別結構域只能識別大腸桿菌外膜和內膜上的特異受體，因此野生型大腸菌素難以拓展成為對抗它種細菌的抗菌素。Related factors of gram ' s dye of coliform
大腸桿菌革蘭氏染色影響因素分析Cloning of bovine interferon - gene in escherichia coli
干擾素基因在大腸桿菌中的克隆In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。The hemagglutinating activity of the fluids was not affected by treatment with 50 mm edta and egta, while it was increased by treatment with 3 - me
經注射雞大腸桿菌后，文昌魚體液中的凝集素對人b 、 o型紅血細胞，兔、草魚和蟾蜍紅血細胞的凝集活性明顯增強。Indication ： for the treatment of bacterial enteritis caused by e. coli, salmonella spp., proteus, dysentery bacillus, an d other organisms susceptible to neomycin
用於治療禽畜的大腸桿菌、沙門氏菌、變形桿菌、痢疾桿菌以及其他對新黴素敏感菌感染引起的腸炎。Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli
小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。Our goal is to express the ricin a chain ( rta ) and its modification rta - kdel ( er - retrival signal ) in escherichia coli
本論文旨在研究蓖麻毒素a鏈蛋白（ rta ）及其修飾體rta - kdel （內質網保留信號肽）在大腸桿菌中的表達。Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2
含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7
為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。The decrease and disappearance of beneficial bacteria number ( as bifidobcteria ) in the intestinal tract in patients with posthepatitic cirrhosis resultin increase of e. coli, which changes as the documinant bacteria, which may lead to raise of endotoxin in the blood
肝炎后肝硬化患者腸道中的有益菌（雙岐桿菌等）減少或消失，大腸桿菌等增加並轉為優勢菌，導致血內毒素升高。The tandem expression of the human salmon chimeric ct gene in e. coli
鮭降鈣素嵌合基因在大腸桿菌中的串聯表達We found that sit variant gene ( slt2vha ) was identified in strains e. coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city, jiangsu province. the primers used for stx2 variant analysis are shown in tablel. genomic dna restriction fragments digested by pstiwere sonthern - blotted and hybridized with an stx2 - specific dna probe. the probe was prepared fromed a 285 - bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel )
2000年在江蘇省徐州市銅山縣腹瀉病患者的糞便標本分離的10株產生志賀毒素的菌株以及從蜣螂腸道分離到的4株產生志賀毒素的大腸桿菌o157 ： h7 ，屬于另兩個pfge型，和1986 、 1987 、 1988年在徐州市腹瀉病患者的糞便標本分離的菌株pfge圖譜不同。The toxin, produced by certain strains of e. coli bacteria, has been found to be responsible for an outbreak of haemolytic uraemic syndrome, a dangerous disease that causes acute kidney failure, in south australia in 1998
此毒素由大腸桿菌特定菌株產生， 1998年澳大利亞南部爆發的溶血性尿毒綜合癥（可導致急性腎衰竭的一種危險的疾病）就是由該毒素引起的。The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody
該法以硝酸纖維素膜為固相載體，包被膜載抗原製成的診斷膜片具有良好的特異性：不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應；膜片具有良好的靈敏性，陽性血清作2 ~ （ - 11 ）稀釋亦能檢出；膜片具有良好的穩定性，在4至少能保存7個月，其靈敏性不變。Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。Vibrio cholerae and enterotoxigenic escherichia coli ( etec ) are major pathogens that evoke acute diarrhea among children worldwide and travelers to developing countries
霍亂毒素與毒素大腸桿菌（ etec ）是引起嬰幼兒和旅遊者腹瀉的主要病原菌。分享友人