腸病菌 的英文怎麼說
中文拼音 [chángbìngjūn]
腸病菌
英文
enterobacterium- 腸 : 名詞1. (消化器官的一部分, 通稱腸子) intestines 2. (用腸衣塞肉、魚等製成的食品) sausage 3. (感情; 情緒; 情感) heart
- 病 : Ⅰ名詞1 (疾病; 失去健康的狀態) illness; sickness; disease; malum; nosema; malady; morbus; vitium...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 病菌 : [醫學] zymad; wog; pathogenic bacteria; germs病菌出口 portal of exit; 病菌傳播 pathogen transmission
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Aetiological isolation and identification of e. coli from chicken and its partial biological characteristics
雞大腸桿菌病病原的分離鑒定和部分生物學特性研究Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Overexpression of sweet potato feathery mottle virus coat protein in e. coli and preparation of its specific antiserum
甘薯羽狀斑駁病毒外殼蛋白基因在大腸桿菌中的表達及特異抗血清的制備Type 1 pili is the important virulence factors on the e. coli in fection in chicken. through the adhering of pili, e. coli adhered on the epidermic cell of aspiratory tract, which was the first step of invading in host
1型菌毛是雞源致病性大腸桿菌的重要毒力因子,在致病過程中介導細菌吸附於雞呼吸道粘膜上皮細胞完成入侵的第一步。It appeared that in that country the disease was caused by an antibiotic-resistant strain of e. coli that was transmitted by the boar.
在那個國家,此病是由一株抵抗抗菌素的埃希氏大腸桿菌菌株引起,由公豬傳播的。This commensal organism is part of the gastrointestinal tract flora and can become extremely virulent, often in the setting of immuno - suppression such as neutropenia, occult malignancy ( commonly caecal ) and poorly controlled diabetes
這種(與人類)共生生物是胃腸道菌群的一部分,可以變得毒性巨大,通常發生於機體免疫受抑制,如中性粒細胞減少癥,隱蔽的腫瘤(一般如盲腸) ,或者控制不佳的糖尿病。Study on drug resistance and molecular epidemiology of enterobacter cloacae infection after liver transplantation
肝移植術后陰溝腸桿菌感染耐藥性及分子流行病學研究Urine specimens were cultured for aerobic gram - negative bacteria, enterococci, coagulase - negative staphylococci and other urinary pathogens
尿液樣本用作培養革蘭陰性需氧菌腸球菌凝固陰性葡萄球菌及其他泌尿道病原體The sucking health care tea of celestial being adjust flu virus 、 spinal cord grey matter inflammation virus and intestines road virus possess the inhibition ; to the first of the ten heavenly stems form even coccus 、 golden staphylococcus 、 pncumonia coccus 、 colon bacillus 、 the green pus bacillus possess the inhibition
仙乳保健茶對流感病毒、脊髓灰質炎病毒和腸道病毒有抑制作用;對甲形鏈球菌、金黃色葡萄球菌、肺炎球菌、大腸桿菌、綠膿桿菌有抑制作用。Other diseases that produce severe diarrhea in neonatal pigs include colibacillosis and enterotoxemia.
引起初生豬嚴重腹泄的其它疾病是大腸桿菌病和腸毒血病。Other diseases that produce severe diarrhea in natal pigs include colibacillosis and enterotoxemia.
引起出生豬嚴重腹泄的其它疾病通常是大腸肝菌病和腸毒血病。2. escherichia coli was the commonest isolate from urine
2 .大腸桿菌是尿液樣本中最常見的病原體。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The results indicated that : jaj could selectively stimulate the reprduction of bifidobacteria in vivo and inhibit the growth of e. coli which is a main parasitic basterium in human intestinal tract ; moreover, jaj could apprarently improve intestinal tract function. in tested group, the mice excreted smoothly and the faecal particles of mice were big and wet, but in control group, the faecal particles of mice were small and dry. lt was suggested that inulin may be the important effective component in jaj which promoted the reprduction of bifidobacteria in vivo. at last, the effects of ja on the bile salt resis tance of bifidobacteria were studied. the test proved that : deoxycholic acid na - salt ( dca - na ) had intensely toxical action on blm and bbm ; adding glucose and fructose in media could decrease the lexical action on bbm. but inulin and jap had not apparent effect
在通過單菌株檢驗和混菌檢驗確立了一種選擇性雙歧桿菌培養基之後,進一步以健康昆明系小鼠為實驗動物,研究了菊芋在動物腸道內對雙歧桿菌的影響,動物實驗結果表明,菊芋汁在體內對雙歧桿菌有選擇性促進生長作用,而腸道中主要條件致病菌?大腸桿菌的生長受到抑制;菊芋中的菊糖成分可能對菊芋在體內選擇性地促進雙歧桿菌生長起了主要作用;此外,菊芋還具有明顯的整腸作用,同對照組相比,飼喂菊芋汁的小鼠排便順利,糞便顆粒大且濕潤。Resistant strains are less likely to develop if a single dose or brief course of a ? lactam antibiotic is administered
大多數常見的病原體為腸道細菌,如大腸桿菌和變形桿菌和假單胞菌。Water quality - detection of human enteroviruses by monolayer plaque assay
水質.用單分子層噬菌斑序列檢測人體腸病毒He xx, wang jl. activity of telomerase and oncogenesis [ j ]. world chinese journal of digestion, 1998, 6 ( 12 ) : 1100
何興祥,王家馬龍,吳捷莉,等.不同病變胃組織端粒酶活性及其與幽門螺桿菌感染的關系[ j ] .胃腸病學, 2000 , 5 ( 1 ) : 19The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體( 100ng l )包被酶標板,加入制備好的肽庫,用tbst洗去非特異結合的噬菌體,加0 . 2mol l甘氨酸-鹽酸( ph2 . 2 ) ,室溫放置10min以洗脫特異結合的噬菌體, 2mol ltris - hcl ( ph9 . 1 )中和后,取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定,其餘噬菌體擴增後用于下一輪篩選,共重復3輪淘洗。Health officials are waiting on test results from california farms and packing plants that could help them pinpoint the source of that deadly e - coli outbreak. 21 states have reported more than 100 e. - coli cases linked to tainted spinach, including a 77 - year - old wisconsin woman who died
衛生官正在等待從佳利福尼亞的農場以及包裝工廠出來的檢測結果,這些結果能夠幫助他們查明致命的大腸桿菌的起源,已經有21個州發現了超過100例與感染有關的病例,其中包括一例死亡病歷:溫斯康新州一名77歲的婦人死於大腸桿菌感染。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明:運用msha法檢測1型菌毛的檢出率為80 ( 36 45 ) , pcr法的檢出率為95 . 5 ( 43 45 ) , pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強;選擇5株優勢血清型雞源致病性大腸桿菌代表株( o _ ( 89 ) , o _ ( 119 ) , o _ ( 141 ) , o _ ( 127 ) )的1型菌毛pila基因的pcr擴增片段經純化后,分別定向克隆到puc18質粒的多克隆位點,構建了含有目的基因片段的克隆質粒,並轉化到dh5株大腸桿菌載體菌中,篩選獲得陽性克隆菌株。分享友人