腺體理論 的英文怎麼說
中文拼音 [xiàntǐlǐlún]
腺體理論
英文
glandular theory-
By the compounds of submandibular gland cells and collagen sponges. we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges, the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge. our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg
在本研究中，以初步探討體外頜下腺細胞與膠原海綿支架相互作用為目的，採用體外分離培養sd大鼠頜下腺細胞，然後接種于膠原海綿支架上體外復合培養的方法；從不同接種細胞濃度對細胞一支架復合物影響，同一接種細胞濃度在不同孔隙率的支架上黏附、增殖的情況及表皮生長因子（ egf ）對頜下腺細胞的促增殖作用，促細胞在支架上黏附等三方面入手，初步研究了頜下腺細胞與膠原海綿相互作用的影響因素，為進一步在體外及體內構建較為理想的組織工程化頜下腺提供理論參數和實驗依據。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Conclusion, misdiagnosis before the operation and insufficient dissection in the operation were the main causes for the reoperation of thyroid carcinoma, so reasonable individual operation method should be chosen when finishing reoperation with positive attitude
結論：術前誤診和首次手術切除范圍不足是造成甲狀腺癌二次手術的主要原因， ，甲狀腺癌應選擇合理的個體化手術方式，二次手術應持積極的態度。
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