膠體條帶 的英文怎麼說
中文拼音 [jiāotǐtiáodài]
膠體條帶
英文
colloform banding-
Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法，用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定，結果表明：抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應，同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在，表明在植物中有類三磷酸肌醇受體蛋白的存在；用免疫膠體金方法，發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。V - belts, composed of high - strength and hign - elasticity tensile body and heat resistance rubber, and is designed for hign - speed and high - temperature application
汽車三角帶由高強度，高彈性的抗張體和耐熱橡膠製成，並被設計成特殊形狀以適合於高速，高溫的條件In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。Nylon is one of the best quality snthetic fibers the rubber in dustry use nowadays. the nylon canvas is woven by nylon fibers both in warp and in weft, it is the most widely used fabric in rubb - er industry, and its outstanding merits are its high abrasion resistance. high fensile strength and good fatigue resistance. connvyor belts with nylon canvas inside have the characteristics of thin belt body, high tensile strength, good shock resistance, good troughability, high adhesion between pli - es, splendid flexibility and long working life. nylon conveyor belts are suitable for medium, long - di - stance and heavy - load transportation of materials, widely used in mining, metallurgical industry and architectural industry, ports and etc
產品說明:尼龍是橡膠工業目前所用合成纖維中性能最好的品種之一，其結構經緯向均為錦綸纖維編織，用途最廣的品種，它最突出的優點是耐磨性好，強度高，耐疲勞性好，尼龍帆布做成輸送帶具有帶體薄，強力高，耐沖擊，成槽性好，層間粘合力大，屈撓性優異及使用壽命長等特點，適用於中長距離，較高載量，高速條件下輸送物料，廣泛用於礦山，冶金、建築、港口等部門。The high purity of genomic dna extracted by tripure isolation reagent was observed. dna agarose gel electrophoresis showed that the genome had a high integrity without degradation. and also, spectrophotometric analysis indicated that the genomic dna had no pollution by protein and rna. 2
培養乳酸乳球菌nizor5 ，收集菌體，用tripurelsolationreagent提取其基因組dna ，瓊脂糖凝膠電泳，紫外顯示儀下可見在點樣孔附近有一條整齊均一的dna帶。Cloning, sequencing and identifying two novel cdna fragments related to chordoma are described in the first part and expression of fhit gene in chordoma is in the second part
Dd一pcr產物應用凝膠系統分離、掃描，切下差異條帶，重新進行pcr擴增純化，擴增體系、條件同dd一pcr 。將純化的。分享友人