芥子酶 的英文怎麼說
中文拼音 [jièzi]
芥子酶
英文
myrosase-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。Thirdly, the changes of peroxidase ( pod ) activity and the analysis of the enzyme spectrum in the morphological different stage in the arabidopsis thaliana were tested. the result indicated that the relation of dose and the activit y was consistent with that of dose and the injury rates of the nutrient organs
通過對離子注入后擬南芥過氧化物同工酶活性的測定和酶譜的分析表明，其活性的大小、酶譜譜帶的有無及深淺與劑量的關系和苗期性狀指標與劑量的關系是一致的。By using arabidopsis thaliana as material, the study had been focused on the ion beam implantation - induced effects and the changes of the configuration. with the rapd method and the analysis of the pod, the mechanism in molecular biology and physiology were stated
以擬南芥為對象做離子注入的物理誘變，觀測記載了離子束注入后供試材料的形態性狀變化，利用rapd方法探討了引起形態性狀誘變的分子機理，結合同工酶分析結果，分析了離子束輻照引起的生理誘變效應。Acbf is a transcription factor that combined with the ac - rich region of the promoter region. it has been cloned at arabidopsis thaliana, nicotiana tabacum and so on
Acbf編碼了結合在苯丙氨酸解氨酶啟動子ac富集區上的一個轉錄因子， acbf基因已經在擬南芥、煙草等植物中得到了克隆。By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively
Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。分享友人