莽草酸途徑 的英文怎麼說

中文拼音 [mǎngcǎosuāntújìng]
莽草酸途徑 英文
shikimic acid pathway
  • 莽 : Ⅰ名詞1. （密生的草） rank grass; thick weeds 2. （姓氏） a surname Ⅱ形容詞1. （魯莽） rash; reckless 2. [書面語] （大） vast
  • 草 : Ⅰ名詞1 （草本植物的統稱） grass 2 （指用作燃料、飼料等的稻、麥之類的莖和葉） straw 3 （草稿） dra...
  • 酸 : 酸構詞成分。
  • 途 : 名詞（道路） road; route; journey; path; way
  • 徑 : Ⅰ名詞1 （狹窄的道路; 小路） footpath; path; track 2 （達到目的的方法） way; means 3 （直徑的簡稱...
  • 草酸 : [有機化學] oxalic acid; oxalate
  • 途徑 : road; avenue; way; channel; approach; pathway; track
  1. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  2. In microorganisms and plants the first step in the aromatic acid biosynthetic pathway is the condensation of pep and e4p, with the release of the phosphate of pep and the formation of dahp

    在細菌和植物中，由公共途徑莽草酸途徑以及三個專一性途徑分別合成苯丙氨酸、酪氨酸和色氨酸三種芳香族氨基酸。
  3. The common biosynthesis pathway of aromatic amino acids includes seven steps from dahp to chorismate acid. for the common pathway, 3 - dehydroquinate ( dhq ) synthase ( encoded by arob ), 5 - enolpyruv - oylshikimate s - phosphate ( epsp ) synthase ( encoded by aroa ), and chorisma - te synthase ( encoded by aroc ] are rate - limiting enzymes

    芳香族氨基酸的合成步驟有七步是共同的，亦即從dahp到分支酸的合成步驟，其中脫氫奎寧酸合成酶（ arob ） 、 5 -烯醇式丙酮酰莽草酸合成酶（ aroa ）和分支酸合成酶（ aroc ）是此代謝途徑的關鍵酶。
  4. The epsp synthase is a necessary enzyme of the shikimate pathway and it is very conservative

    Epsp合成酶是莽草酸途徑的一個關鍵酶，其成熟蛋白在進化中極為保守。
  5. Shikimic acid pathway

    莽草酸途徑
  6. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  7. The product chorismate of this pathway is precursor to the synthesis of the aromatic amino acids phenylalanine, tyrosine, and tryptophan as well as other essential aromatic secondary metabolites such as ubiquinone, menaquinone and vitamin k. phosphoenolpyruvate and shikimate 3 - phophate are converted to 5 - enolpyruvlshimimate - 3 - phosphate and phosphate

    芳香族氨基酸苯丙氨酸、酪氨酸、色氨酸和其它一些必需代謝物如泛醌、維生素k2類、維生素k的起始物是莽草酸途徑所產生的分枝酸。
  8. We amplified ppsa and tkta from e. coli k - 12 by pcr and constructed recombinant plasmids of them in pbv220 vector containing prpl promoter. because of each gene carrying pl promoter, four productions of ligation were obtained, that are ppt -, ppt -, ptp -, and ptp -. the results of sds - page demonstrated that the bands at 84kd and 73kd were more intensive than the same ones of the controls

    3 .從大腸桿菌中克隆了莽草酸途徑關鍵酶基因aro刁、 aroc 、 arob及整體代謝調控基因csra及其側翼序列;從質粒pet28a中克隆了kan抗性基因; 4 .構建了基因盒子aroaar口carobkan ，在其前面加入了強啟動子ptac ，初步證明了pta 。
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