菌毛蛋白 的英文怎麼說
中文拼音 [jūnmáodànbái]
菌毛蛋白
英文
pilin-
In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子( bp53片段)和脫毛蛋白酶全基因( ap )進行融合,然後將重組基因(命名為bpap )插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中,構建成表達質粒psu一bpap 。The blood of each rabbit was collected at days 0, 7, 14, 2l, 30. the antibody titers were evaluated by k - agglutination test. the results showed that lower agglutination titers was observed at day 7 and up to higher levels over 8000at day 21 induced by the vaccine of recombinant fused protein
將融合基因工程菌株在營養肉湯中培養,離心後上清用0 . 1mmgcl _ 2沉澱,離心,提取表達產物。經sds - page電泳證明表達產物為纖毛蛋白與il - 2的融合蛋白。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態;應用原子力顯微鏡掃描,比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別;用透射電鏡觀察細胞壁完整性,以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變,並通過實驗分析多種物質和因素對潛生體形成的影響。
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