The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿
菌lba4404細胞中,然後採用葉盤法,在該農桿
菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因
的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性
的高抗病毒
的蛋白質。
The nitrogen fixation in alpine meadow ecosystem is mainly accomplished by anaerobic nitrogen fixing bacteria. both ammonification and nitrification are the highest in 0 cm 10 cm soil depth
從不同植被類型土壤
的表層中各生理群
菌數
的平均值來看,反硝化細
菌的數量最高,嫌氣性自生固氮
菌次之,再次為氨化
菌和硝化
菌。
The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria
二、土壤微生物總dna
的提取和純化方法研究為了採用不依賴于培養
的16srdna分析
的方法研究有機磷農藥長期污染對土壤微生物群落結構
的影響,建立了從土壤中提取總dna
的方法,並通過改進使適合於對革蘭氏陽性
菌的提取。
At the earliest developmental stage of the myxomycetes, the un - nucleate amoeboid or swarm cells of all the species are similar to each other in the aspects of morphology, structure and habit
在孢子萌發后
的最初發育階段中,所有黏
菌的單核黏變形體和游動胞在形態、結構和習性等方面均相似。
1. ecological effects of long - term organophasphate pesticides contamination on soil microflora the long - term effects of organophosphate pesticides contamination on soil microflora were investigated in the present study. little difference in total counts of bacteria, actinomycetes and fungi was observed between the contaminated and the non - contaminated soil. compared with the control there were a slight decrease in total counts of free - living nitrogen - fixer and denitrifying bacteria and a significant increase in those of ammonifying and ammonia - oxidizing and nitrifying _ bacteria in the methylparathion contaminated soil
一、甲基對硫磷長期污染對土壤微生物
的生態學效應研究了有機磷農藥甲基對硫磷長期污染對土壤微生物
的影響,實驗表明:土壤細
菌、放線
菌、真
菌總
的數量影響不大;自生固氮
菌和反硝化細
菌數量減少;氨化細
菌、亞硝化細
菌、硝化細
菌的數量在污染土壤中卻有所增加;與對照土壤相比,污染土壤呼吸作用下降了29 . 93 ;氨化作用和硝化作用強度得到增強。
Daqu, a microbial product containing multiple enzymes and bacterial species, its aroma - producing substances mainly come from proteins and fat of daqu - making materials and the degradation of amylum and those substances are composed of amino acids, fatty acid, polyose and its polymers etc
摘要大?是一種富含多酶多
菌的微生態製品,大?復合曲香物質來源於制曲原料中
的蛋白質、脂肪以及澱粉等
的降解,其復合曲香由氨基酸、脂肪酸、多糖及其聚合物等多種物質共同構成。
Under mixed culturing conditions, it was observed that bacterial number rapidly incre ; ised soon after the lysing of host cells. on the contrary, while the non - host cyanobacterium ( i. e. anabaena flos - aquae ) was incubated in the mix culturing system, the breeding of the bacteria could be inhibited. it may be suggested from the result above that cyanophage could bring about the changes in microorganism populations
調查結果顯示, 19個採集
的水樣中有6個含有裂解織線藻
的噬藻體,而且水樣未經濃縮即能裂解宿主,說明噬藻體在淡水中分佈較廣泛,裂解性也較強;混合培養條件下
的研究結果表明,噬藻體裂解宿主后,細
菌數量快速增加,而當培養系統中有非宿主藻類存在時,細
菌的增殖則受到非宿主藻
的抑制,說明噬藻體可以顯著改變系統中微生物
的種群結構。
Methods the metablic organic acid of 60 strains of 6 species of oral residential anaerobes were tested by gas chromatography qualitatively and quantitatively
方法利用氣相色譜法對6種厭氧
菌的國際參考
菌株
的代謝有機酸進行了定性定量測定。
An infectious disease characterized by cycles of chills, fever, and sweating, caused by the parasitic infection of red blood cells by a protozoan of the genus plasmodium, which is transmitted by the bite of an infected female anopheles mosquito
瘧疾一種傳染性疾病,癥狀為周期性地感到冷、熱和發汗,病因是寄生於紅血球
的一種瘧原蟲屬原生動物,這種動物通過已感染病
菌的雌性瘧蚊傳播。
Five chitosan samples ( 3. 8 10 ^ 5, 7. 8 10 ^ 4, 4. 8 10 ^ 4, 1. 7 10 ^ 4, 2. 3 10 ^ 3 ) were used to investigate their activity in vitro against phytopathogenic fungi, which caused anthracnose on garden plants such as euonymus japonicus, ophiopogon japonics and magnolia grandiflora, and rot on fraxinus hupehensis
採用平板法研究了殼聚糖系列樣品對大葉黃楊炭疽病、沿階草炭疽病、廣玉蘭炭疽病和對節白蠟腐爛病等庭院植物病原真
菌的體外抗
菌性能。
They say you do not have to use special antibacterial soap
沒必要用專門殺菌的肥皂。