菌落培養 的英文怎麼說

中文拼音 [jūnlàpéiyǎng]
菌落培養 英文
colong count
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 落 : 落名詞[方言] （北方對蓮花落的俗稱） a kind of folk song
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  1. The virulent strains had a polysaccharide capsule and formed smooth colonies on agar medium.

    有毒菌株具有多糖莢膜它在瓊脂培養基上形成光滑的菌落。
  2. The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria

    二、土壤微生物總dna的提取和純化方法研究為了採用不依賴于培養的16srdna分析的方法研究有機磷農藥長期污染對土壤微生物群落結構的影響，建立了從土壤中提取總dna的方法，並通過改進使適合於對革蘭氏陽性菌的提取。
  3. Most grow best and form pycnidia earlier on ma, and pda is slightly not fitful for pycnidia growth of the kind of fungi. none of the species can produce ascocarps in 60 days. esterase isoenzymes of 12 strains of rhytismataceae were studied by polyacrylamide gel electrophoresis and clustering analysis of upgma and 12 strains were classified into 4 groups when correlation coefficient is 3. 1

    結果表明大多數種在ma平板上菌落營養生長最好，產生分生抱子器及分生抱子的時間較早、能力較強； oa也是可選擇的培養基之一：而p a較不適宜於該類菌物的營養生長和分生抱子器產生。
  4. There are also some estimates of the efficiency of litter decomposition by basidiomycetes in pure culture.

    也有一些關于純培養擔子菌分解落葉層效力的估計。
  5. On pda medium, some isolates are protruding while others are effuse, some isolates are pink while some isolates are white or yellowish milky, and textures of most of the isolates are loose while some are hard and close

    Implicatum的11個分離物的遺傳多樣性進行了研究，多數分離物間存在遺傳多樣性，在pda培養基上，一些分離物菌落突起，另一些分離物菌落凹陷。一些分離物菌落的正面為白色，而另一些分離物菌落的正面為粉紅色。
  6. Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle

    首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。
  7. Take a portion of the same colonies as under 3. 8. 1 by means of a wire and stab inoculate a glucose agar tube. incubate at 37 ? 1 c for 24 h

    用接種針挑取與3 . 8 . 1實驗相同的菌落的一部分接種至葡萄糖瓊脂試管. 37 ? 1 c培養24小時。
  8. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  9. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  10. Potato dextrose agar and grain medium were also used to identify fungi which were not determined by the primary culture. fungi were all secondarily cultured on sabouraud medium to observe the colony ' s texture, colour, growth rate, surface status and reverse pigment. the fungi should be examined by microscope to inspect their microscopic structure from 7th day to 21st day

    使用的培養基有沙氏培養基、土豆培養基、真菌試驗培養基和5種種子培養基，連續培養4周，並隨時觀察菌落的色彩、生長速度、表面狀態、背面顏色等，並從第7天?第21天連續鏡檢以檢查真菌的顯微結構，綜合菌落形態和顯微結構，以確定真菌的種屬。
  11. Capacity evaluation of different media for isolating predominant phenol - degrading bacteria from activated sludge with community structure - specific dna probes

    微生物群落結構探針雜交評價不同培養基從活性污泥分離優勢菌群的能力
  12. The free living and particle - attached bacteria groups are significantly different in term of species composition. plate culture strains are different from dominant field groups. this result proved the insufficiency of traditional cultural methodology

    適合於在平板培養條件下生長的類群並非湖水中的優勢類群，這一結果進一步證實了傳統的培養方法在分析水體細菌群落生物多樣性方面的不足。
  13. The results show that most isolates produce enough condia. which are similar to those directly received from the hosts, when they grow on the twa + w culture media for about 1 - 2 weeks, under 18 - 22 and alternative nuv light for 12h and in dark for 12h. but we should try several different kinds of culture media to produce the natural condia for some special species

    總結如下： （ 1 ）適宜培養基及適宜培養條件：使用自來水洋菜麥稈培養基（ twa + w ） ，近紫外燈照射下， 18 22下， 12h光照與12h黑暗交替培養時，大部分菌落生長較好；但對個別的種也應使用多種培養基，選擇最接近自然狀態的培養基和培養條件。
  14. First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d

    首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。
  15. Colonies the beach water samples are tested for

    經培養后的大腸桿菌菌落圖片
  16. Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy, including numerical taxonomy, rep - pcr fingerprinting, 16s rdna pcr - rflp. the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp. showed great diversity. ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains, the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid. the other strains were slow - growing strains, their single colony size was less than 1 mm after 7 days incubated on yma medium at 28. they can produce alkali

    本研究以從我國四川、河南、安徽和湖南等地分離的32株葛藤根瘤菌為研究對象，以20株已知種的根瘤菌為參比菌株，採用數值分類、 rep - pcr指紋分析、 16srdnapcr - rflp指紋分析等現代根瘤菌分類技術，初步研究了葛藤根瘤菌的生物多樣性和分類地位，結果表明：葛藤根瘤菌在生長速率上表現出多樣性，菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生長較快， yma培養基上28培養2 - 3天後，單個菌落直徑大於1mm ，具有產酸能力，是快生型葛藤根瘤菌；其餘待測葛藤根瘤菌生長較慢， yma培養基上28培養7天後，單個菌落直徑小於1mm ，具有產堿能力，是慢生型葛藤根瘤菌。
  17. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  18. They incorporate chemical compounds that, following inoculation and incubation, produce a characteristic change in the appearance of bacterial growth and / or the medium surrounding the colonies, which permits differentiation

    它們納入化合物，因此在接種與培養后細菌生長的外觀或者在菌落的四周形成一種具有特色的改變，這種改變具有分辨能力。
  19. Water quality - enumeration of viable micro - organisms - colony count by inoculation in or on a nutrient culture medium

    水質.活微生物計數.營養培養基中或上面的單菌落計數
  20. Their morphological and physiological characteristics were observed through the strains colony morphology, size, color, growth rate, texture, and spores

    用察氏平板培養基分離菌株，根據菌株的菌落形態、大小、顏色、生長速率、質地、生長培養基顏色變化以及菌絲體和抱子的形態特徵進行鑒定。
分享友人
分享到 Line

分享到臉書