薄層掃描 的英文怎麼說
中文拼音 [bócéngsǎomiáo]
薄層掃描
英文
thin slice scan-
5. detection : the samples were extracted with ethyl ether. the lidocaine in the sample was qualitated by rf and a tlc scanning spectrometry, quantitated by a tlc scanning chromatography and a two points external standard method or a standard curve method
5 、利多卡因檢測方法:樣品堿化處理后，乙醚提取，薄層掃描檢測，根據利多卡因的rf值結合光譜掃描圖定性，外標兩點法或標準曲線法定量。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Determination of the ephedrine content in yaotongning with gracile scanning
薄層掃描法測定腰痛寧中麻黃堿的含量Determination of the tetra hydro palmatine in guanshu tabel by tlc scanning
薄層掃描法測定冠舒片中延胡索乙素含量Determination of triglycerides in oil of brucea javanica by tlc - scanning
薄層掃描法測定鴉膽子油中三油酸甘油酯含量The determination of protocate chualdehyde in teblete guanshu by hplc
高效薄層掃描法測定雷公藤搽劑中雷公藤內酯醇含量Determination of emodin in kangyanning granules by tlc - scanning
雙波長薄層掃描法測定梔核清肝膠囊中大黃素含量It owns more than 50 precision instruments, such as lc - ms, gc - ms, hpce, hplc, gc, ft - ir, uv, tlcs, aa, etc. there are about 15 thousand chinese and foreign books on relative specialities and more than 140 sorts of chinese and foreign journals in the library
擁有液-質及氣-質聯用儀原子吸收分光光度計毛細管電泳儀高效液相色譜儀氣相色譜儀紅外分光光度計薄層掃描儀全自動溶出儀等大型精密儀器150多臺件。Content assay of the ground parts of eomecon chinantha hance
薄層掃描法測定血水草地上部分中羽扇豆醇乙酸酯的含量Determination of matrine in mongolia medicine chagantang by tlc - scanning
薄層掃描法測定蒙成藥查干湯中苦參堿的含量Determination of puerarin in tongmai oral solution by tlcs
薄層掃描法測定通脈口服液中葛根素含量Determination of emodin in qinggan granules by tlcs
薄層掃描法測定清肝顆粒中大黃素的含量In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。Tlc - scanning determine astragaloside i of shenqi anoqing grahule
薄層掃描法測定參芪腦清顆粒的黃芪甲苷含量Determination of astragaloside in anshen ningkang capsule by tlc - scannan
薄層掃描法測定安神寧康膠囊中黃芪甲甙的含量Determination of three active ingredients in food additive sucrose fatty acid ester by tlc scanning
薄層掃描法測定蔗糖脂肪酸酯中的各組成酯Quantitative determination of isoimperatorin in shangtongning tablet by tlc - scanning
薄層掃描法測定傷痛寧片中異歐前胡素含量Determination of dracorhodin in xuejie from different places by tlc scanning method
薄層掃描法測定不同來源血竭中血竭素含量Determination of emodin and chrysophanol in granules for relieving inflammation in women fukexiaoyanlinghao granule by hplc
薄層掃描法測定婦炎克顆粒劑中鹽酸小檗堿及大黃素的含量Chest routine scan
胸部薄層掃描分享友人