蛋白結合分析 的英文怎麼說

中文拼音 [dànbáijiēgěfēnxī]
蛋白結合分析 英文
protein binding analysis
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 結 : 結動詞（長出果實或種子） bear （fruit）; form （seed）
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  • 結合 : 1 （發生密切聯系; 聯合） combine; unite; integrate; link; binding; coalition; cohesion; connectio...
  1. Cloning and sequence analysis of the cabps gene from echinococcus granulosus

    鈣結合蛋白基因的克隆和序列分析
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  4. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  5. Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function

    進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。
  6. Then. we analyzed the immunogenic proteins of aeromonas hydrophila, and identified 5 immunogenic proteins by the use of 2 - de and western - blot

    採用2 ? de與western ? blot相結合，對嗜水氣單胞菌的鼠免疫原性蛋白進行分析，鑒定了5個免疫原性蛋白。
  7. Methods : after cultured scs combined with fn were grafted into hemi - resected lumbar spinal cords of adult rats, we used surface recording of spinal cord evoked potential ( scep ) and image analysis system to compare the latent period of scep and count the axon number between the therapeutic group and the control group

    方法：將體外培養的雪旺細胞結合纖連蛋白植入大鼠半切損傷的脊髓內，用脊髓誘發電位及圖像分析方法比較治療組和對照組誘發電位潛伏期，再生軸突計數及二者間的關系。
  8. This study demonstrated that the arabidopsis f - box protein coil associated with atcul1, atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes. also, we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull. ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2

    用表達融合蛋白myc - ask2的擬南芥為材料，以- myc抗體進行免疫共沉澱分析發現， myc - ask2蛋白可以與coi1蛋白一起免疫共沉澱，但是不能與ask1蛋白免疫共沉澱，表明coi1蛋白與ask2蛋白，但是不能同時與ask1結合形成scf ~ （ coi1 ）復合體。
  9. 二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system

    二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。
  10. Female mice serum and vaginal secretion antibodies, their fertility and the pathological changes were determined. on the other hand, the effects of the anti - serum against synthetic peptide on the sperm - egg interaction during ivf and the location of p3 peptide in sperm were watched. the main results and conclusions were as follows : 1 ) the new antigen p3 peptide which harvested from the 430a peptide synthesizer were verified by the mass spectrograph and hplc, that the ratio of mass / charge of the synthetic peptide was equal to the molecular weight in theory and its purity was above 95 %

    本研究的主要結果和結論如下：一、經抗原分子設計，在430a自動肽合成儀上合成的新抗原p3 ，經質譜儀分析證明其荷質比與理論一致；純化后，其純度大於95 ；將新抗原與klh偶連后免疫小鼠，經westernblot證實其誘導的特異性抗體可識別小鼠、大鼠、人睪丸組織中分子量約為22kd和55kd左右的蛋白質。
  11. Advance on bacterial quorum - quenching enzymes

    酶標白桂木凝集素糖蛋白結合特性的分析
  12. The researchers combined proteomics ( the study of proteins ), the latest in mass spectrometry technology and the best analytical methods from the field of bioinformatics ( the use of computers and statistics to analyze and find patterns in scads of data )

    研究者將蛋白組學（研究蛋白質） ，最新的質譜測定技術及最好的生物信息學分析方法（應用電腦及統計學分析並檢驗大量數據類型）相結合。
  13. After breast feeding, their descendents were fed with the same water, then killed them on the 1st, 8th, 15th, 22nd, 30th day after birth respectively and took out of the brains, stored at - 70. ( 2 ) measurement of the activity of pkc in brains : in cytoplasma ; took out the samples, cut the hippocampi into pieces and added buffer ( glucose 0. 32mol / l, hepes ph 7. 5 ), then shattered with ultrasound, centri - fuged at 27000g for ih at 4ti, moved the supernant into a new tube

    本研究以細胞信號轉導途徑為線索，分別探討了pkc 、 erk 、 nos1等信號分子及即早基因（ c - fos ， c - jun ）在慢性鉛暴露小鼠腦細胞中的活性、蛋白表達及mrna表達的改變，以及鉛暴露小鼠記憶行為的變化，並將細胞中信號分子的改變與記憶功能改變結合進行分析以尋找一些內在規律。
  14. There were some degration in the purified protein, but gst - ap - 2 a still had the dna binding activity in the gel shift assay. the gst - testin and gst - antn1 were used for immunolize rabbits

    雖然所提取的融合蛋白gst - ap - 2出現較多降解，但是電泳遷移率變動分析顯示其仍然具有良好的dna結合活性。
  15. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  16. A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity

    報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑（溴化十二烷基三乙銨/十二烷基硫酸鈉）分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基，研究蛋白質在雙水相體系中的親和分配.結果表明，在該體系中，表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成，不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性
  17. Conference contents ： to discuss the structure determination of membrane protein and protein complexes, and the recent progress of different methods to analyze and determine biological structures using electron microscopy

    探討三維電子顯微學在膜蛋白/蛋白復合體結構解析的應用以及三維電子顯微學在解析蛋白晶體中分析方法的最新進展等。
  18. The scfco, " ubiquitin - ligase complexes are required for jasmonate response in arabidopsis ( l ) arabidopsis coi is required for all the jasmonate - regulated response. it encodes a protein containing leucine - rich repeats and a degenerate f - box motif these structural features are characteristic of f - box proteins that function in ubiquitin ligase complexes for the ubiquitylation of substrate - proteins targeted for degradat1on

    以表達融合蛋白flag - coi1的擬南芥為材料，用- flag抗體進行免疫共沉澱分析發現， coi1蛋白可以與ask1 、 ask2 、 atrbx1和atcul1蛋白結合形成scf ~ （ coi1 ）復合體，並發現其中的atcul1蛋白包括未修飾和修飾的兩種形式。
  19. Identification and analysis of a mouse gene homologous to human hepatitis b virus pre - s1 protein - binding protein using the bioinformatics method

    1蛋白結合蛋白小鼠同源基因的生物信息學分析
  20. The tools and analytical methods that biochemists use to dissect biological problems. analysis of the mode of action and structure of regulatory, binding, and catalytic proteins

    生化學家用來解決生物學問題的工具及分析方法.對作用方式和蛋白質調控、結合和催化模式的結構分析
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