融合內蛋白子 的英文怎麼說

中文拼音 [rónggěnèidànbáizi]
融合內蛋白子 英文
fused intein
  • 融 : Ⅰ動詞1 （融化） melt; thaw 2 （融合; 調和） blend; fuse; be in harmony Ⅱ形容詞[書面語]1 （長遠; ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 內 : 名詞1. （內部; 里頭; 里邊） inner; inside; within 2. （妻子或妻子的親屬） one's wife or her relatives 3. （姓氏） a surname
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 子 : 子Ⅰ名詞1 （兒子） son 2 （人的通稱） person 3 （古代特指有學問的男人） ancient title of respect f...
  • 融合 : fuse; mix together; anastomosing; reconcile; harmonize; compromise; amalgamate; coalesce; coalesc...
  • 白子 : albino
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  3. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  4. Doing so revealed a hydrophobic meaning water - repellent tip that allows the viral harpoon to latch on more securely to the cell membrane, which is also hydrophobic. it also provided researchers with more insight into the dramatic structural change that the f protein undergoes while performing its task. when not in use, the f protein looks like a mushroom, and its hydrophobic tip is folded into a compact form, safely hidden inside the cap

    在沒有碰到人體細胞時， 「副流感病毒5型」細胞表層的「融合蛋白」看起來就像一個蘑菇，防水頂端嚴密封閉，且安全地藏在蘑菇帽子下面當病毒接觸到人體細胞時，蘑菇帽子會突然破裂開來，防水頂端也像魚叉一樣暴露出來，並陷入人體細胞膜內。
  5. The protein has the immunogenicity of human calcitonin verified by western blot analysis. after optimization of condition, fusion protein was concentrated by ultrafiltration and cleaved by factor x a, then recombinant novel hct was obtained

    表達條件優化后，可溶性蛋白的表達量占胞內總蛋白的10 。經超濾濃縮后，融合蛋白在xa因子作用下產生重組的新型降鈣素，進一步分離純化得到該蛋白的初級純品。
  6. Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh, the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form. both of the products in the two strains showed biological activity, but the former is 2 times higher than the latter. the hyuc dna fragment was inserted into ppic3. 5k plasmid to construct the ppic3. 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion

    結果表明， sds - page分析在50kd處有一較強的表達帶，融合有分子伴侶的重組菌株bl21 （ de3 ） pt221 - hyuh與非融合表達的重組菌株m15 pqe60 - hyuh相比，乙內酰脲水解酶的表達量低一些，但其表達蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表達蛋白則主要以包含體形式存在，且前者的酶活性是後者的2倍多。
分享友人
分享到 Line

分享到臉書