蟋蟀科 的英文怎麼說
中文拼音 [xīshuàikē]
蟋蟀科
英文
cricket-
In the present paper, some investigations on loxoblemmus doenitzi ( gryllidae ) are reported. in order to discover the meiosis of spermatomeres and spermiogenesis, slice - pressing method and improved phenol - fuchsin dyeing method are used. in the course of meiosis, primary spermatocytes directly enter metaphase ii, not through telophase i and prophase ii
本論文以直翅目、螽斯亞目、蟋蟀總科、蟋蟀科的多伊棺頭蟋( loxoblemmusdoenitzi )為材料,採用壓片法-改良苯酚品紅染色,研究了精子發生過程中的減數分裂階段和精子形成,發現初級精母細胞第一次減數分裂后不經過末期和前期直接進入中期。2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin
等( 1995 )利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種, allonemobiusfasciatus和a . socius ,並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖;國內田英芳、鄭哲民( 20of )首次將rapd技術運用於蟋蟀總科的分子系統學研究中,採用2種引物對7種蟋蟀進行了基困組dna多態性研究,並應用upg問a法構建樹狀圖,椎測系統發生關系Among 54 arbitrary primers tested, only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae
本研究經用54種隨機引物對57種蟋蟀的rapd擴增分析得出以下結論: 1 )採用rapd技術,首次較系統地對中國蟋蟀總科7科26屬共57種蟋蟀進行了系統學研究。在所試用的54種隨機引物中篩選出可擴增出清晰可辨且穩定的rapd圖譜的引物分別為:蟋蟀科9種、嶺蟋科9種、樹蟋科12種、結蟋科12種、蛛蟋科13種、鐵蟋科13種、貌蟋科13種。2 ) the method of proteinase k isolation and the method of ctab isolation had been compared carefully while extracting the genome dna from crickets. the result showed the method of proteinase k isolation of total dna was more suitable for the extraction of total dna from insects of grylloidea
2 )在提取蟋蟀總科昆蟲的基因組dna時,比較了ctab法和蛋白酶k法兩種方法,結果顯示蛋白酶法更適用於蟋蟀總科昆蟲的基因組則a的提取。Thermocycled pcr samples were resolved electrophoretically on 1. 5 % agarose gels and taken photos using a polaroid camera. the statistical results were analyzed by the spss software, and the cluster figures were obtained. conclusions could be drawn from the study as following : 1 ) the molecular systematics of 57 species of crickets, which belong to 26 genera 7 family in grylloidea, had been studied by the approach of rapd
本項研究在依據外部形態分類鑒定及前人工作的基礎上,採用rapd技術,通過對蟋蟀總科7科26屬57種蟋蟀基因組dna的rapd圖譜的比較研究,在分子水平上探討這些類群的分類地位和親緣關系,為豐富蟋蟀總科的分子系統學研究,並為進一步完善蟋蟀總科的分類系統,揭示其系統發育及演化提供分子水平的依據。In this paper, the approach of rapd was used to study 57 species of crickets, which belong to 26 genera, 7 families, in order to discuss their taxonomy status and gentic relationships in molecular level, and enrich the research of molecular systematics in grylloidea. the genome dna of the 57 species were extracted by the method of proteinase k isolation of total dna
蟋蟀總科的分子系統學的研究也主要集中在個別種種內或種間mtdna多態性方面及個別種同工酶的研究上,而利用rapd技術進行蟋蟀基因組dna多態性方面的研究在國內外更少,國外僅見chu , jAbstract : based on the investigation of the grylloidea insect resources and the evaluation of cricket ' s main nutrient components, the ways to develop and utilize crickets scientifically was discussed from the angle of nutritive health
文摘:在調查了解濟南地區蟋蟀類昆蟲資源及其主要營養成分評價的基礎上,從營養保健的角度,探討了科學開發利用蟋蟀資源的途徑。分享友人