蟲體抗原 的英文怎麼說
中文拼音 [chóngtǐkàngyuán]
蟲體抗原
英文
somatic antigen-
Lots of pharmacological and clinical experiments indicate that artemisinin is high effective with low toxicity. evidences from many laboratories suggest that the antimalarial function of artemisinin depends on the cleavage of the endoperoxide by binding to intraparasitic heme
雖然目前還沒有直接證據表明蟲體內蛋白質烷化是蟲體致死的原因，但烷化蛋白與青蒿素類藥物抗瘧作用有關是很明顯的。A rabbit was infected with a cloned yntatl, blood was collecting from from the rabbit every 3 days after infection within 30 days, 10 clonal trypanosome populations were gotten, infecting a new rabbit by the last non - cloned trypanosome population. repeated above all, thus infected 5 rabbits sequentially. twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia )
用伊氏錐蟲雲南水牛單克隆株yntat1感染兔，感染后30天內，每3天從兔血中分離錐蟲並單蟲克隆，最後一個未單蟲克隆的蟲株感染另一隻兔，重復以上操作，這樣順序感染5隻兔子，共獲得50個單克隆錐蟲種群（ tp ） ，經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體（ vats ） 。Guan et al ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 ofschistosomajaponicum, which is an internal image anti - idiotypic antibody of gut associated antigen ( gaa ), its antibody isotype is igm. np30 has a partial cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ). np30 molecule has been researched on many aspects
管曉虹等（ 1991 ）建立了一株日本血吸蟲單克隆抗獨特型抗體np30 ，已證實為腸相關抗原（ gaa ）的內影像抗獨特型抗體，其抗體同型為igm ，與可溶性蟲卵抗原（ sea ）及膜相關抗原（ maa ）有部分交叉反應。Guan xiaohong, et al. ( 1991 ) established a strain of monoclonal anti - idiotypic antibody np30 of schistosoma japonicum, whice is an internal image antigen of gut associated antigen ( gaa ), its antibody isotype is igm. np30 has a partial cross - reaction with soluble egg antigen ( sba ) and membrane associated antigen ( maa )
管曉虹等建立的日本血吸蟲單克隆抗獨特型抗體（ anti - id ， ab _ 2 ） np30是腸相關抗原（ gaa ）的內影像anti - id ，與可溶性蟲卵抗原（ sea ）有交叉反應，可替代蟲源性抗原用於血吸蟲病免疫診斷和抗病疫苗的研究。The second part, a renewable piezoelectric immunosensor is developed for the antibody of schistosoma - japonicum ( sjab ). after incubating 32 kd molecular antigens of schistosoma japonicum ( sjag ) on the qcm by applying the immobilization above, the nonspecific sites on the immunosensor are sealed by using bsa and nrs together. the immunosensor can detect the sjab with the linear range of 0. 54 ~ 32. 50 ug / ml
以感染兔血清為檢測對象，採用聚電解質吸附固定法，將日本血吸蟲分子抗原（ siag32kd ）固定於石英晶振表面，再以牛血清白蛋白（ bsa ）和正常兔血清（ nrs ）聯合封閉晶振上非特異性活性位點，可在0 . 54 32 . 50ug ml范圍內檢測感染兔血清中日本血吸蟲抗體。At first, study on antigenic variation in trypanosoma evansi from a clonal stock in rabbit was carried out
首先進行的是伊氏錐蟲一個單克隆株在5隻兔體內連續抗原變異的研究。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。The doctor would prescribe a course of anti - malarial drugs with other supportive measures. the patient should complete the whole course of medication to ensure clearance of the malaria parasites
醫生會處方抗瘧疾藥物和其他支援性治療，患者須根據醫生的吩咐完成整個抗瘧疾藥物的療程，以確保將體內的瘧原蟲徹底根除。The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male
Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。It has been confirmed that np30 has sensitizing effect on formation of hepatic egg granulomas in mouse model for hepatic egg granuloma of schistosoma japonicum ; immunization with np30 in kunming mice, c57bl / 6 mice and goats obtained worm reduction of 50. 46 %, 41. 67 % and 42. 78 %, respectively. np30 possesses effects of both anti - fecundity and anti - embryonation immunity on female worms of s. japonicum. moreover, np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis ( the diameter, area and volume of egg granuloma were all significantly less than those of control ; the contents of type i, iii of collagen and fibronectin were also significantly less than those of control )
已對np30分子進行了較為廣泛的研究，應用小鼠日本血吸蟲肝肉芽腫模型證實np30對蟲卵肉芽腫的形成具有致敏作用；對感染宿主（昆明種小鼠、 c57bl / 6小鼠和山羊）具有較好的免疫保護作用（減蟲率分別為50 . 46 、 41 . 67和42 . 78 ） ；用np30主動免疫小鼠具有抗雌蟲生殖產卵和抗卵胚發育的雙重功效；另外，還對血吸蟲病肉芽腫和肝纖維化有明顯的負調節作用（蟲卵肉芽腫的直徑、面積和體積均明顯小於對照組，肝組織、型膠原及纖維連接蛋白含量均低於對照組） 。In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。In this study we had used the sensor to detect antibody - antigen reactions of fasciolsis, nd, ibd and eds. it is proved the sensor is fitted to use in detecting antibody - antigen reactions of these diseases. the results of detecting antibody - antigen reactions of fasciola gegatica, nd, ibd and eds by using the sensor basically conform to that with standard samples
在實驗研究中，我們先後應用電化學免疫傳感器測試了大片形吸蟲，雞新城疫（ nd ） 、雞傳染性法氏囊病（ ibd ） 、雞減蛋綜合征（ eds ）等的抗原抗體反應，結果與我們先前初步設定的判斷標準基本相符。Study on chicken egg yolk antibody used in the pathogenic detection of mice infected by toxoplasma gondii
抗體用於弓形蟲感染小鼠病原學檢測的研究Ts87 gene fragment was picked out by screening adult t. s cdna library using sera of rabbit raised against soluble antigen of t. s and using sera of rabbit infected artificially with t. s. the ts87 fragment was ligated to vector pcdnas. l, which contains a cmv promoter / enhancer
本研究採用通過免疫篩庫得到的旋毛蟲抗原基因片段ts87與載體pcdna3 . 1連接。該載體含有人類巨細胞病毒（ cmv ）的高效啟動子和增強子，是一種外源基因在哺乳動物細胞內高效表達的理想載體。In order to find out regularity of antigenic vareation, a cloned population of trypanosoma evansi stock yntatl was infected sequentially to 5 new zealand rabbits during 150 days. competing the ablity of anti - trypanosoma evansi between 3 different antibody bank derived from rabbits, setting up by different means
隨后根據所獲得的抗原變異體（ vatvariableantigentype ） ，建立抗伊氏錐蟲兔血清抗體庫?原混血清庫、蟲體血清庫、 vsg （ variablesurfaceglycoprotein ）血清庫，並對抗體庫的抗蟲效果進行檢測。分享友人