表異構酶 的英文怎麼說

中文拼音 [biǎoyìgòu]
表異構酶 英文
epimerase
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 構 : Ⅰ動詞1 （構造; 組合） construct; form; compose 2 （結成） fabricate; make up 3 （建造; 架屋） bui...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle, low cost and high benefit. though traditional breeding technology has been used for a long time. now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known.

    關于花色結構基因查爾酮異構酶chi （ chalconeisomerase ）基因對花色改變的直接報道很少，因此，本論文選用了chi基因為目的基因，以純深紅色和純白色矮牽牛（ petuniahybidavilm . ）為材料，研究了chi基因的共抑制和反義抑制以及表達產物增加對花色改良的作用，並在花色改變植株中首次觀察到花器官變異。
  3. Expression of linoleate isomerase gene from lactobacillus reuteri pyr8 in escherichia coli and assay of its bioactivity

    8亞油酸異構酶基因在大腸桿菌中的表達與活性檢測
  4. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  5. Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch

    利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
  6. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  7. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  8. 2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence

    異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。
  9. Abstract : biological invasions are a continuous feature of a non - equilibrium world, ever more so as a result of accidental and deliberate introductions by mankind. while many of these introductions are apparently harmless, others have significant consequences for organisms native to the invaded range, and entire communities may be affected. here we provide a survey of common models of range expansion, and outline the consequences these models have for patterns in genetic diversity and population structure. we describe how patterns of genetic diversity at a range of markers can be used to infer invasion routes, and to reveal the roles of selection and drift in shaping population genetic patterns that accompany range expansion. we summarise a growing range of population genetic techniques that allow large changes in population size ( bottlenecks and population expansions ) to be inferred over a range of timescales. finally, we illustrate some of the approaches described using data for a suite of invasions by oak gallwasps ( hymenoptera, cynipidae, cynipini ) in europe. we show that over timescales ranging from 500 10000 years, allele frequency data for polymorphic allozymes reveal ( a ) a consistent loss of genetic diversity along invasion routes, confirming the role of glacial refugia as centres of genetic diversity over these timescales, and ( b ) that populations in the invaded range are more subdivided genetically than those in the native range of each species. this spatial variation in population structure may be the result of variation in the patchiness of resources exploited by gallwasps, particularly host oak plants

    文摘:生物入侵是不均衡世界的一個永恆話題，尤其是當人類有意或無意地引入物種后.很多引入顯然是無害的，但另外一些則有著嚴重的後果，會給入侵地的生物以至於整個生物群落造成影響.本文總結了分佈區擴張的常見模式，概述了它們對遺傳多樣性和種群結構式樣所造成的影響.描述了如何根據以一批遺傳標記所得到的遺傳多樣性式樣來推斷入侵途徑，來揭示伴隨擴張選擇和漂變在形成種群遺傳樣式中的作用.本文對日益增多的群體遺傳學方法進行了總結，這些技術可以用來在不同的時間尺度上推斷種群規模所發生的巨大變化（瓶頸效應及種群擴張） .最後，我們以歐洲櫟癭蜂（膜翅目，癭蜂科，癭蜂族）一系列入侵的數據為例對一些方法進行了說明.從500 10000年的時間尺度上，多態的等位酶位點上等位基因頻率的數據表明: 1 ）遺傳多樣性沿入侵路線呈不斷下降的趨勢，支持了冰河期避難所作為遺傳多樣性中心的作用; 2 ）入侵地區的種群與該物種原產地的種群相比，遺傳上的分化更為強烈.這種種群結構在空間上的變異可能是被櫟癭蜂開發的資源尤其是櫟樹寄主在斑塊上出現變異的反映
  10. Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis

    分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。
  11. In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica

    對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna （包括可能與復甦能力有關的cdna ）和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明，牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等） 、調節基因的級聯作用（ vp3 ， mad3樣基因等） 、結構基因產物調節細胞結構（包括細胞質膜）在脫水脅迫中的穩定性等。
  12. Immunohistochemistry staining showed that induced different - passage mscs expressed neurofilament ( nf ) and neuron - specific enolase ( nse ). special nissl bodies were obvious in the neuron - like cells by histochemistry staining

    免疫組化法檢測發現這些神經細胞強表達神經絲蛋白（ neurofilament ， nf ）和神經元特異性烯醇化酶（ neuron - specificenolase ， nse ） ：組織化學法檢測可見神經元特有結構尼氏體。
  13. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  14. In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr

    流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關，為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制，本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象，用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響，用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。
  15. The expression of topoisomerase ii of cultured cells treated low dose ionizing radiation decreased and then returned as time went. and become outstanding as radiation dose and frequency were added. inhibitor of topoisomerase ii could cause chromosome non - dis juction in mitosis and meiosis. and the co - effect of inhibitor of topoisomerase ii and ion

    05 ） ，具有隨照射次數增加而增加的趨勢；小劑量電離輻射可以引起拓撲異構酶a表達變化，隨照射后時間延長先下降后回升，隨照射劑量和次數的增加，變化更加明顯；拓撲異構酶11a抑制劑可引起有絲分裂和減數分裂時染色體的不分離；拓撲異構酶a抑制劑和電高輻射的協同作用使染色體不分離更加明顯。
  16. Localization and expression of three nitric oxide synthase isoforms in the cochlea of guinea pigs and the effects in the hearing process of inner ear

    一氧化氮合酶3種異構體在豚鼠耳蝸的定位表達及其內耳聽覺過程中的作用
  17. In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules

    研究發現呷基因的調控區存在多種轉錄因子結合位點，功能主要涉及組織特異性表達調控，細胞生長發育相關基因的表達調控，刺激反應基因的表達調控等； ndrgz蛋白在結構上屬于a小水解酶類折疊，折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似，提示ndrgz蛋白具有一定酶活性，可能參與細胞抗氧化應激反應，維持細， an ） armtbffiofbfochmilsyn ） mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡，參與內外源有毒物質的代謝等。
  18. In the present experiment studies, an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ), cyclooxygenase - 2 ( cox - 2 ), glial cell line - derived neurotrophic factor ( gdnf ), caspase - 3 mrna and protein after brain injury in rats. immunocytochemical staining, western blotting, nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna, cox - 2 mrna, gdnf mrna, and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )

    本實驗從自行設計大鼠腦損傷落體打擊器開始，先行建立了一個便於觀察和施加處理因素、控制性好、重復性好的動物模型，選用30g擊錘從25cm高處下落，沖擊應力d為355 . 09kpa ，打擊大鼠右頂部，造成中等程度的閉合性腦損傷，從病理形態學、組織超微結構觀察及微管相關蛋白- 2 （ microtubuleassociatedprotein2 ， map - 2 ） 、環氧合酶- 2 （ cyclooxygenase2 ， cox - 2 ） 、膠質源性神經營養因子（ glialcellline - derivedneutrophicfactor ， gdnf ） 、 caspase - 3基因及蛋白表達的時間性變化，詳盡系統地闡述腦損傷后各指標變化的時間規律性及表達差異可能的形成機制。
分享友人
分享到 Line

分享到臉書