表達和分泌 的英文怎麼說

中文拼音 [biǎodáhéfēnbì]
表達和分泌 英文
rat rantes
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 泌 : 泌名詞[書面語] （湧出的泉水） gushed spring water
  • 表達 : deliver; express; show; voice; convey; communicate
  1. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  2. There are distributed in different regions and with different densities in the brain, so it can be supposed that il - 2 has physiological functions in these different regions. for instance, il - 2 has the nutrition function on the neurons and neuroglias, and affects the release of neurotransmitter. it has been tested that il - 2r can be expressed in oligodentrocytes and microglias

    Il - 2r在中樞神經系統中有廣泛表達，且不同腦區il - 2r的分佈及密度不同，推測il - 2因而發揮不同的生理功能，如其能影響營養神經元和神經膠質的功能，影響神經遞質的分泌等。
  3. Owing to the common expression of egf in various organs of mouse, it could be deduced that the role of egf was mainly autocrine and paracrine actions, and its endocrine role was also not excluded owing to its high expression abundance in submaxillary gland

    由於egf在小鼠各器官中表達的普遍性，可以推測egf的作用以旁分泌和自分泌作用為主，但它在頜下博士學位論文「 tnegfalelittin 」嵌合基因構建及有關基因重組表達的研究等器官中的高表達表明其也具有內分泌作用。
  4. To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated

    但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。
  5. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  6. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  7. The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased

    結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。
  8. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  9. An array of regulatory proteins have been found, which inhibit the formation of central enzymes involved in early stages of the complement activation pathway. these include membrane cofactor protein ( mcp cd46 ), decay - accelerating factor protein ( daf cd5 5 ), complement receptor 1 ( cr1, cd35 ), as well as cd59, which inhibits formation of the membrane attack complex during later stages. these regulatory factors are widely expressed and abundant on many cells, and in fluids of reproductive system

    目前發現，機體多種細胞以及生殖系統的體液中表達和分泌豐富的補體調控蛋白，包括作用於補體活化早期階段的cd55 、 cd46 、 cd35和作用於補體活化終末階段的cd59 ，它們分別通過抑制補體活化過程中關鍵的c3 、 c5轉化酶和抑制形成膜攻擊單位，抵抗補體對自身組織細胞的攻擊。
  10. The secretive expression of scfv fusion proteins were confirmed by sds - page and western blot analysis in cell split products and condensed supernatant

    建系細胞裂解產物和濃縮的培養上清經sds page及westernblot分析，證實了scfv融合蛋白的分泌性表達。
  11. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  12. Effects of candida albicans on expression of adhesion molecules and secretion of cytokines by human vascular endothelial cells

    白念珠菌對人血管內皮細胞表面黏附分子表達和細胞因子分泌的影響
  13. Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein

    表達重組蛋白angiostatin ：用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞，用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析，依此確定最適的感染復數（ moi ）和感染時間，以達到重組蛋白表達水平最適化，而後大規模進行重組蛋白的表達， sds page用來分析重組蛋白， westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。
  14. It was recognized as a brain - gut peptide since the peptide and its receptor exist in the central nervous system. previously, we demonstrated that motilin and motilides activates cells in paraventricular nuclei ( pvn ) reflected by increased gastric motility and induction of the immediate early gene c - fos in conscious rat, which suggests central motilin participates in the regulation of gastric motility. it was reported in 2001 that ghrelin is an appetite - stimu latory signal from stomach with structural resemblance to motilin

    胃動素（ motilin ， mt ）是由小腸上段粘膜內分泌細胞合成並分泌的22個氨基酸組成的腦腸肽，調節消化間期胃運動， pvn內給予胃動素和胃動內酯（ motilides ）可增強清醒大鼠胃運動，中樞微量注射胃動素可誘導pvn中立即早期基因c - fos的表達，提示中樞胃動素參與胃運動的調控，人ghrelin和胃動素之間有36的相似性，而且二者的受體之間有50的相似性，因而有人稱ghrelin為「胃動素相關肽」 。
  15. . . nearly all aspects of cell life are regulated by reversible protein phosphorylation. some examples include metabolic processes, gene regulation, cell cycle control, transport and secretory processes, the organization of the cytoskeleton, and cell adhesion

    蛋白可逆磷酸化幾乎可以調節所有的細胞活動，包括代謝過程、基因表達調控、細胞周期調控、運輸和分泌過程、細胞骨架的形成和細胞粘連。
  16. Regulated upon activation normal t cell expressed and secreted

    細胞表達和分泌的趨化蛋白
  17. Objective to investigate the effect of liuwei dihuang soft capsule and ginkgo leaf tablet on serum regulated upon activation, normal t cell expressed and secreted rantes in the patients with diabetes mellitus type 2

    目的探討中藥六味地黃軟膠囊和銀杏葉片對2型糖尿病患者血清受活化調節由正常t細胞表達和分泌的趨化蛋白regulated upon activation ， normal t cell expressed and secreted ， rantes水平的影響。
  18. Objective to investigate the effect of liuwei dihuang soft capsule and ginkgo leaf tablet on serum regulated upon activation, normal t cell expressed and secreted rantes in the patients with diabetes mellitus type 2. methods forty patients with early stage dm2 were randomly assigned to two groups, 20 in each group

    目的探討中藥六味地黃軟膠囊和銀杏葉片對2型糖尿病患者血清受活化調節由正常t細胞表達和分泌的趨化蛋白regulated upon activation ， normal t cell expressed and secreted ， rantes水平的影響。
  19. Unfortunately, tumor cells can secrete immunosuppressor factors ( such as il - 10, tgf - p, vegf ) to interfere with dc maturation and function. to overcome tumor - mediated inhibition of dc maturation and function in vivo, immunopotentiating cytokines ( such as il - 7, il - 12 ) were used for gene - modification of dc, which has proven to be an efficient way to treat cancer in animal models

    雖然有直接瘤內注射dc誘導抗腫瘤免疫效應的報道，但腫瘤局部往往是一個處于高度免疫抑制狀態的微環境，腫瘤細胞分泌的可溶性介質（如ill 、 tgf卜vegf等）能抑制dc的分化成熟，下調dc表面共刺激分子的表達和iliz的分泌，從而抑制dc的抗原提呈功能，亦對t細胞功能有顯著的抑制作用。
  20. The results suggested : the differential gene expression in the adrenal gland under acute hyperglycemia / hypoglycemia was related to blood glucose ; these genes might regulate blood glucose by influencing the metabolism and secretion of hormones, cytoskeleton constitution and signal transduction in the adrenal gland ; bbs4 gene might participate in glucoregulation via the activities of adrenal medulla ; the differential genes expressed under acute vs chronic hyperglycemia might play a basic role of the structural and functional changes in the adrenal gland, lead to the process of chronic complications of diabetes or accommodate to the status of hyperglycemia

    結果提示：腎上腺基因差異性表達和血糖變化有關；在急性高低血糖情況下，這些基因可能通過影響腎上腺內激素的分泌和代謝、細胞骨架構成和信號轉導來調節血糖； bbs4可能參與血糖調節，其作用可能和腎上腺髓質的活動有關；慢性高血糖情況下的腎上腺差異表達不同於急性高血糖，這種表達差異可能與腎上腺慢性的結構和機能改變相關，或參與了糖尿病慢性並發癥的發生發展、高血糖狀態的調節。
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