表達實體 的英文怎麼說

中文拼音 [biǎodáshítǐ]
表達實體 英文
expression substance
  • 表 : Ⅰ名詞1 （外面;外表） outside; surface; external 2 （中表親戚） the relationship between the child...
  • 達 : Ⅰ動詞1 （暢通） extend 2 （達到） reach; attain; amount to 3 （通曉; 明白） understand thoroughly...
  • 實 : Ⅰ形容詞1 （內部完全填滿 沒有空隙） solid 2 （真實; 實在） true; real; honest Ⅱ名詞1 （實際; 事實...
  • 體 : 體構詞成分。
  • 表達 : deliver; express; show; voice; convey; communicate
  1. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  4. Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree

    為此，本實驗從銀杏leafy同源基因的克隆入手，分析其雌雄株lfy基因結構差異，構建lfy基因的植物正義反義表達載體，建立矮牽牛遺傳轉化體系，以研究銀杏lfy同源基因的功能，同時建立了銀杏組織培養體系，為銀杏的遺傳轉化和提早開花結果奠定基礎。
  5. On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

    本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。
  6. By analyzing the puissance, experience, body and happiness language, i find that the really meaning of the female fashionable periodical is a kind of contradictive expression. it is a sign of status, but also is a reversed discrimination ; it wants be out of the traditional culture, but also in it ; elegant feminine temper is also in the charge of the masculine society ; happiness is based on the expenditure of body and substance. now the contradictive expression of feminine language is becoming the most important problem in the development of the female fashionable periodical

    筆者通過對女性時尚期刊權力、經驗、身體、快樂話語的解構和分析，發現女性時尚期刊的表達陷入了一定的話語矛盾和困境:是身份認同的標志，卻又隱含著反向歧視;經驗話語的「反文化」表達，實質上是建立在「泛文化」基礎上對傳統文化的部分回歸;優雅可人的女性氣質，說到底卻帶著男性規訓的深深烙印;快樂話語的傳播，是以對消費主義的追隨和女性身體的消費為前提的。
  7. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  8. The native expressed product from e. coli bl21 ( de3 ) strain, however, showed weak activity against tmv. gp609 and zwemu were inserted into pemu - mcs - n, an expression vector for monocotyledon. and rhxjb was inserted into pkylx71 : 35s2

    將dna一8001連接到pet一sa表達載體上，在大腸桿菌blzi （ de3 ）菌株中實現天然表達，表達產物對tmv的抑制效果很差，其原因可能是c一末端延伸序列的存在抑制了抗tmv活性的發揮。
  9. The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition

    本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。
  10. Through the approach of investigative questionnaire and ability examination, considering both teachers " teaching practice in senior high school and students " need in analyzing and resolving problem, taking the method of fixing the quality and quantity, we make a demonstration research. at last, we draw a conclusion that the 19 manifestations of chemistry ability can be reduced to three levels : the basal level, called repeating thinking, includes distinguishing and judging the chemical fact and relations of quantity in chemical reaction, comparing and summarizing the chemistry fundamental knowledge ; innovative thinking level includes illogical thinking and logical thinking. illogical thinking mainly contains intuition, association and space imagination of micro - particle

    通過調查問卷、能力測試等途徑，結合教師在中學化學的教學實踐和學生在分析、解決問題的實際需要，採用定性與定量結合的方法，進行實證研究，得出結論：化學學科能力的19種表現形式歸納為3個層次：最基礎的再造性思維能力層次包括對化學事實和化學反應中量的關系的識別和判斷、對化學基礎知識的比較概括；以培養創新能力為核心的創造性思維層次包括化學直覺思維、聯想、對微觀粒子的空間想象為主的非邏輯思維和靈活運用已學的化學知識通過分析、綜合地解決問題為主的邏輯思維；化學實驗能力層次是化學學科能力的最高層次，它以思維能力為基礎，集中體現了化學教學的基本特徵，實驗能力層次包括：選用實驗方法和設計實驗方案，對實驗的評價，研究和處理實驗事實、資料、數據，發現規律，表達實驗及其結果，最終解決問題的能力和發散思維。
  11. In this study, in order to examine the dynamics of tip [ ca2 + ] i together with the dynamics of tip - localized f - actin in vivo, a kind of double labeled material was constructed. the ca2 + and actin microfilaments of arabidopsis pollen tubes were labeled by cameleon and gfp - mtalin respectively

    本研究以擬南芥花粉管為材料，通過轉基因技術，將分別標記ca ~ （ 2 + ）和微絲的兩種熒光蛋白cameleon與gfp - mtalin在花粉管中同時表達，實現活體花粉管中ca ~ （ 2 + ）與微絲的同時標記。
  12. The resulting plasmid, named prok - sod2, was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation. the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ), several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination

    本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那黴素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。
  13. We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm

    Eif - 5a的hypusine修飾是其活性和功能發揮所必需的，我們通過pcr方法實現了hypusine位點的定點突變，並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。
  14. It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed

    的序列正確，經酶切鑒定證實成功地構建了綠色熒光蛋白基因融合表達載體。
  15. In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted

    第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ，在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。
  16. After one of them was expressed in e. coli bl21, the product was purified by ni - nta column

    經酶切鑒定證實原核表達載體ptat爿ascfv構建成功。
  17. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  18. The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin

    本實驗採用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
  19. It can become a reality to the liver and also represent the thought of self - centralization from the relationship between self ( liver ) and world ( dwelling ) from the ideas of body upon the taiwanese vernacular dwellings

    從身體到時間、空間、天地、萬物等皆可從身體的特有認知方法出發，並且完成一個可分述，卻也能形成整體性的總體認知；身體與宇宙之間創建出的秩序與符號表達，實時常規文化內涵融合的體現。
  20. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
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