表達性行為 的英文怎麼說
中文拼音 [biǎodáxìnghángwéi]
表達性行為
英文
expressive behavior- 表 : Ⅰ名詞1 (外面;外表) outside; surface; external 2 (中表親戚) the relationship between the child...
- 達 : Ⅰ動詞1 (暢通) extend 2 (達到) reach; attain; amount to 3 (通曉; 明白) understand thoroughly...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 行 : 行Ⅰ名詞1 (行列) line; row 2 (排行) seniority among brothers and sisters:你行幾? 我行三。where...
- 表達 : deliver; express; show; voice; convey; communicate
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These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta. microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis, adaptation or primary metabolism for examination of gene expression in the sublines. the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0. 8028 - 0. 9028, while those of a78 - 5 are 0. 8897 - 0. 9302
選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍晶元膜,並對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 : 88 : 22等培養再生植株進行基因表達的微陣列檢測,結果表明: ( 1 )三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。The existing theoretical analyses have n ' t refelcted this true mechanism of the force transfering in fixed segment of anchor bar. for the sake of studying the working course from elastic to elastoplasticity slide better, this paper put forward shear stress - displacement curve, and solve the internal force distribution along anchor bar by the basic equntion. at the same time, this paper analysize the difference of columniform fixed segment and the segmeng with anchor slab at the foot of anchor bar
為了更好的研究錨桿在不同荷載作用下,錨桿的工作狀態從彈性到彈塑性發展的整個過程,本文進行了錨桿受力的模型實驗研究,根據實驗結果提出了剪應力與位移的彈塑性本構關系表達式,並根據錨桿受力的基本方程,求解得出了錨桿在不同工作狀態下從彈性到彈塑性滑移的全過程中錨桿的內力分佈。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Output can be obtain, next we use our designed the wavelet soft threshold to select result of the wavelet transform, finally, we give the selected result reversal wavelet transform. it is obvious : the wavelet soft threshold is important to improve the quality of the gray image processing. i give the donoho wavelet soft threshold a modified value method, which has a relation with ratio of signal - noise. i made full use of discrete hop field single feedback neural network, and nonlinear steady of automatic system at last, i obtained a steady limited ring, give the energy function an order differential a optimal
我的思路是:改造現有圖形結構,先建立能反映信噪比大小且含待定參數k的表達式,然後通過離散型h0pfi舊單層反饋神經網路,再結合前邊的混合濾波器構成一個非線性控制系統,寫出對應的網路函數,利用相平面法和李雅普諾大穩定性的判據,得到一個穩定的極限環,從而確定出參數卜的范圍,進而再對原來的小波軟閾值進行修正,用修正後的值作為小波閾值。Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。As it ' s proprietary, a large numbers of bibliography resource ca n ' t be provided to common readers of internet. along with the development of network technology, it has a instancy need to conversion marc format to the information that the browser can recognize. the language xml not only can expresses the metadata, but also can show the content of data. lt can provide individuation page layout
Marc格式是目前圖書館館藏資源的主要表示格式,由於其專用性,使得目前大量的書目資源無法通過一般的搜索引擎向internet用戶提供,隨著網路技術的不斷發展,迫切需要將marc格式的數據轉換成常用瀏覽器所能識別的信息,為大眾提供服務,可擴展標記語言xml不僅可以表達元數據,而且可以揭示數據的內容,提供個性化的頁面顯示,用xml語言對marc數據進行描述是解決這一問題的有效途徑,文章提供了marc數據的xml表示方法和實現途徑。Collisonal quantum interference ( cqi ) was observed in the intramolecular rotational energy transfer in the experiment of the static cell, and the integral interference angles were measured. to observe more precise information, the experiment in the molecular beam should be taken, from which the differential interference angle can be obtained precisely. in this paper, the theoretical model of cqi is described in an atom - diatom system in the condition of the molecular beam, based on the first - born approximation of time dependent perturbation theory, taking into accounts the anisotropic lennard - jones interaction potentials. the method of observing and measuring correctly the differential interference angle is presented. the changing tendencies of the differential interference angle with the impact parameter, velocity, and et al. are discussed
分子內部轉動傳能的靜態池實驗觀察到了碰撞量子干涉效應( cqi ) ,並且測得積分干涉角,為了獲得更加精確的分子內部轉動傳能的碰撞量子干涉效應信息,實驗就必須要採用分子束實驗進行.本文理論上採用各項異性相互作用勢,應用含時微擾理論的一級波恩近似,假想在分子束實驗的條件下,建立在原子-雙原子分子體系中碰撞量子干涉的理論模型.理論上推導出微分干涉角具體表達式,通過計算定性地討論了微分干涉角隨著碰撞參數、速率等的變化趨勢,同時初步探討了實驗的正確觀測途徑,得出了採用分子束進行實驗觀測的實驗方法,為進一步進行分子束實驗提供了理論基礎,對實驗的進行起到了一定的借鑒作用This thesis will rely on previous studies of instructions on the general rules and language functions, and modification of teacher talk to carry on a multi - layer study on junior middle school teachers " instructions with aspect of linguistic forms and language functions in chinese efl classrooms. after study on the transcriptions of 30 lectures ( 10 lectures are given by in - service teachers, 10 lectures are given by pre - service teachers and 10 lectures are given by the winners in fine - quality classroom competitions ) of efl classroom of junior middle schools in china, the major findings are : ( 1 ) the teachers likely use some devices with respect to prosody ( temporal variables ), lexis, syntax and discourse to simplify and clarify the complex instructions to match the requirement of junior middle school students " listening comprehension. ( 2 ) there are about three factors that cause the ineffective instructions
本文在前人的關于教師語言的調整,指令語及其表達功能的研究基礎上以何安平教授建立《中學英語教育語料庫( mstm ) 》中的近17 . 7萬字的子語庫《英語課堂教學語料庫( msee ) 》為研究語料,選擇了其中30節初中英語課為研究對象,對教師指令語的言語形式及其語言功能進行了較為深入的描述分析,發現: ( 1 )在音律層面,中國中學英語教師習慣川停頓來放慢語速或在一些難詞之前做停頓以便達到讓學生理解的要求:詞匯層面,教師慣用一些表達方式來給指令語;句法層面,教師也是盡量用簡單句來簡化指令語;語篇層面,初中英語課堂上教師常用一些信號詞來起始解釋性指令語,並少用宏觀語篇標記詞來幫助學生理解,但卻常用微觀語篇標記詞來吸引學生的注意力。In summary, we should investigate the more efficient cultural system for mammary orgnoids in vitro for enriched more mammary epithelial stem cells, and to screen the expression profiles in mammary epithelial stem cells to discover the specific marker for mammary epithelial stem cells in order to utilize the model of mammary epithelial stem cells to regenerate transgenic mammary glands in cow or sheep for human health, to uncover the mechanism of organogenesis, and differentiation
在此之前,仍然只能以造血幹細胞的特異性表達抗原scal - 1作為一個標記,進行類乳腺幹細胞的分析和分選研究。進一步研究類乳腺幹細胞的特異性表達譜,以期能夠找到類乳腺幹細胞的特異性表達抗原,分離得到類乳腺幹細胞,作為發育生物學和再生生物學研究的模型。Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用,並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平, mcf - 7 adr呈強陽性,而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % , mcf - 7細胞系細胞陽性率為1 . 8 % 。We have reported that squalene, a natural fish oil, is a potential preventive agent against sodium arsenite - induced sister chromatid exchanges and micronuclei in chinese hamster ovary - k1 cells
以達成熟地步,因此使得探討環境毒物暴露引發的差異性基因表達成為可以進行的研究。我們已開始以Injected group, 0. 1 % saccharin ( 1. 5 ~ 2ml / rat, in 5min ) intraoral infused group and cta group. the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ), lateral part of mediodorsal thalamic nucleus ( mdl ), ventroposterolateral thalamic nucleus ( vpl ), ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry. in behavioral experiment, 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i. p
為探討cta形成過程中enk的作用,本實驗用成年雄性sd大鼠35隻,分為空白對照組、生理鹽水( 2體重)腹腔注射組、 0 . 15mlicl溶液( 2體重)腹腔注射組、 0 . 1糖精溶液口腔灌流組( 1 . 5 - 2ml只, 5min )和cta建立組,採用免疫細胞化學方法,觀察了亮腦啡肽( lek )陽性神經元在大鼠腦內的分佈情況,並比較了各組大鼠丘腦外側背核( ld ) 、丘腦內側背核外側部( mdl ) 、丘腦腹后外側核( vpl ) 、丘腦腹后內側核( vpm )以及丘腦網狀核( rt )等5個腦區內lek表達水平的差異;另外將成年雄性sd大鼠18隻,分為正常cta建立組以及在cta建立前或cta建立后阿片受體拮抗劑納洛酮( 2mg kg體重)腹腔注射組,對內源性阿片樣物質對于cta建立和保持的影響進行了行為學研究。Mood : a set of verb forms or inflections used to indicate the speaker ' s attitude toward the factuality or likelihood of the action or condition expressed
語氣用來強調說話人對表達的行為或條件的真實性或可能性所持有態度的一系列動詞形態或變化形式。Coli have been performed in this study. engineering bacteria, screened for efficient expression of pheromone 3, express the proteins in type of insoluble ( inclusion ) and soluble forms, respectively
本實驗對游仆蟲信息素在大腸桿菌中的表達進行了系統的比較與分析,篩選獲得了有效表達信息素的工程菌株,表達產物分別為包涵體與可溶性蛋白。Boolean expressions are intended precisely to express runtime true or false properties ? properties that at any point in the execution may hold or not hold
布爾表達式的設計意圖就是表達運行時值為真或假的屬性,在某個時刻判斷此屬性的值就可以決定執行是否繼續。Boolean expressions are r nintended precisely to express runtime true or false r nproperties ? properties that at any point in the execution may hold or r nnot hold
布爾表達式的設計意圖就是表達運行時值為真或假的屬性,在某個時刻判斷此屬性的值就可以決定執行是否繼續。After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin, the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay. the assays demonstrated that the purified ntfs proteins exhibited normal activity, which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis
最後,對重組表達蛋白經glutathlonesepharose4bmicrospincolumn親和純化后,進行大鼠腎上腺嗜鉻瘤細胞( p2 )神經營養因子的活性鑒定,發現其能夠誘導神經細胞分化產生突觸,即具有預期的生物學活性,表明所獲表達蛋白為大熊貓ntIntroduced infected by the turnip mosaic virus. there are many useful objective genes can be used in vegetable genetic transformation, but the research work of chinese cabbage genetic transformation is little. the reaserch on transforming chinese cabbage using agrobacterium - med ated method has n ' t been report at home and abroad. ln the test, tumv - cp gene was transferred into chinese cabbage ( brassica campestris l. ssp. pekinensis ) via agrobacterium - mediated method. a high effective regeneration and genetic transformatin system has been established, the detection by the method of molecular biology, has proved that the regenerative plants are transgenic plants and the target genes have been expressed transgenic plants partly. meanwhile transgenic progenies were traced and investigated so that heredity, stability and expression of target gene were researched. the virus resistant, stable plants were expected to obt ain so that theoretical base can be established for chinese cabbage breeding by gene engineering
利用農桿菌介導法轉化大白菜抗病基因的研究工作在國內外未見報道。本課試驗採用農桿菌介導法將tumv - cp基因導入大白菜中,建立了高效的大白菜離體再生、遺傳轉化體系,並對轉基因植株進行分子生物學檢測,證實得到的再生植株為轉基因植株,目的基因已在部分植株上表達。同時,對轉基因植株的後代進行檢測,分析該基因所控制性狀的遺傳穩定性以及基因表達情況,為大白菜基因工程抗病育種提供理論依據。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。Let ' s just say : renegades for the particularity of individual human experience and against both the uniformity of “ correct language ” that imposes preformed orders on live thinking and also against moral and religious laws that unnecessarily regulate individual behavior and expression
我們不妨這么說:反叛是為了維護個體經驗的獨特性;是為了反對正統語言的一致性,這種一致性把既定的秩序強加在發展變化的思想上;反叛是為了反對道德和宗教體系,它們不必要地對個人行為和表達進行約束。分享友人