裂解胞 的英文怎麼說

中文拼音 [lièjiěbāo]
裂解胞 英文
tmema
  • 裂 : 裂動詞[方言] （東西的兩部分向兩旁分開） open
  • 解 : 解動詞（解送） send under guard
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  1. The phage has replicated and the donor cells have lysed.

    噬菌體已復制和給體細胞已發生裂解。
  2. The toxin may be formed within the pathogen and released when it dies ( endotoxin ), or secreted through its cell wall ( exotoxin )

    在病原體內形成，當病原體裂解時被釋放的毒素為內毒素；由病原體通過細胞壁向外分泌的毒素為外毒素。
  3. After reproduction of the viral nucleic acid the host cell usually undergoes lysis ( lysogeny )

    病毒核酸復制后寄主細胞通常要經歷裂解過程（溶源狀態） 。
  4. About 30 minutes after infection the bacterial cell breaks open(lyses)and the phage particles are released.

    侵染后約30分鐘，細菌細胞裂解，釋放出噬菌體顆粒。
  5. 2. stage of absorption : the fragments of the target tissue s cells will be gradually absorbed, glial cells will be generated, blood vessel will be blocked, intradermal cells will be generated and new capillary vessel will be formed in the following year after the stage of putrescence

    2 .吸收期：壞死期后至照射一年，所照射組織細胞裂解碎片被逐漸吸收，膠質細胞增生，血管閉塞，內皮細胞增生，並出現新生毛細血管
  6. Halocin c8 appeared to have a very wide activity spectrum, including most haloarchaea and even some haloalkaliphilic rods, but it showed no inhibitory activity against bacterial strains tested. when a sensitive strain of halorubrum saccharovorum atcc29252 was exposed to halocin c8, the treated cells swelled at the initial stage, the cell wall appeared to be nicked and the cytoplasm was extruded out afterwards, and the whole cell was eventually completely lysed

    將敏感菌株halorubrumsaccharovorumatcc29252的細胞用嗜鹽菌素c8處理后，採用電鏡觀察，發現處理2小時后細胞開始腫脹、膨大，接著在細胞的兩端出現裂口，細胞內容物開始向外溢出，隨著時間延長，裂口越來越大，發生這種變形的細胞也越來越多，最後，幾乎所有的細胞完全裂解。
  7. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  8. Biological activity was determined by egf dependent balb / c 3t3 cell line and with mtt colorimetric assay. extracts of the recombinant virus - infected and mock - infected cells, haemolymph of the recombinant virus infected and mock - infected silkworm larvae could all support the proliferation of balb / c 3t3 cell. this phenomena implied that there were some egf - like growth factors in the haemolymph of normal silkworm larvae, which could enhance the proliferation of the cell line

    用小鼠balb c3t3成纖維細胞和mtt法測定表達產物的促細胞增殖作用，發現重組病毒感染家蠶細胞72小時的胞內樣品與正常家蠶細胞裂解物，以及重組病毒感染4天的蠶血淋巴與正常蠶血淋巴均具有相似的促細胞增殖作用，甚至野生型病毒感染的細胞裂解物和蠶血淋巴也有一定的細胞促生長作用，提示家蠶系統本身可能含有能促進細胞生長、類似於egf的細胞因子。
  9. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  10. To obtain a heterogeneous random mixture of dental enamel epithelial and pulp mesenchymal cells, the pig teeth were broken into tiny pieces and then further dissolved using enzymes

    為了取得各種隨機混合的牙釉質上皮細胞與牙髓間質細胞，他們將豬的牙齒裂解成微小碎片，然後再利用酵素加以解離。
  11. So some methods suitable to large plasmid extraction, including lysis by sds and a method from a literature, were used to try to extract the large plasmid from the strain cell. the lysis reactions in these two methods are gentle, so the large plasmid cannot be injured in the lysis process, opposite to lysis by alkali. it would be helpful to keep the integrality of the large plasmid during the extraction

    因此我們採用了適合於大質粒提取的sds法，和文獻中應用於硝基苯降解性質粒的提取方法，來嘗試對菌株細胞進行質粒提取，這兩種方法裂解反應溫和，不會像堿裂解法那樣，在裂解過程中損壞質粒，可以實現質粒提取的完整性。
  12. 2n methods 1 ) sample preparation the supernatant of cultures were digested with identical volume lysis buffer at 100 for 15 min. dna was isolated for use as template

    二、實驗方法1 、標本處理取hcmv低傳代分離株細胞培養上清液與等量裂解液混勻后，煮沸15分鐘，提取dna ，作為擴增模板。
  13. The secretive expression of scfv fusion proteins were confirmed by sds - page and western blot analysis in cell split products and condensed supernatant

    建系細胞裂解產物和濃縮的培養上清經sds page及westernblot分析，證實了scfv融合蛋白的分泌性表達。
  14. The plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only

    對於野生菌株的質粒提取，需要選擇合適的提取方法，我們首先採用了細菌細胞質粒提取常用的堿裂解法，但是未取得理想的效果，只得到了許多破碎的質粒dna片段。
  15. Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod

    方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。
  16. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  17. To circumvent these deficits, novel antigen - delivery systems utilizing cytokine gene - modified tumor cells and dc or fusion of dc with tumor cells have resulted in induction of antitumor immunity. however, this u approach is difficult in some cases ( for example in breast cancer ) because only rarely has it been possible to isolate enough viable tumor cells from an individual to prepare the vaccine

    為了克服上述缺陷，有學者採用滅活的腫瘤細胞、腫瘤抗原提取物（包括腫瘤細胞裂解物、 it na和洗脫肽等）沖擊致敏dc或將腫瘤細胞與dc融合后再回輸體內以激發機體的抗腫瘤免疫應答，也取得了較好的療效。
  18. The study in vitro of anti - hepatoma immunity induced by dendritic cells pulsed with the lysate of autologous hepatoma cells

    自體肝癌細胞裂解物致敏的樹突狀細胞誘導抗肝癌免疫的體外研究
  19. Tadashi shuibakawa, min - sheng hung, and masao washizu *, " laser - induced local decomposition of a chromosome and dna stretching by electroosmotic flow ", the 10th symposium on chemistry and micro - nano - system, takamatsu, japan, 25 - 26 november ( 2004 )

    洪敏勝* ，朱菀婷，郭婷勛，陳柏嘉，艾群， "細胞裂解與dna萃取之研究" ，第九屆奈米工程暨微系統技術研討會，臺南， 11月10 - 11日（ 2005 ）
  20. A sds - iso - propanol method suitable for tea plant, which was plentiful of tea polyphenols, had been developed using a modification of chen darning ' s method from different sample storage conditions such as fresh, dry and frozen shoots. it was a quick, easy, economical and effective method. the tactics were as follows : before the cell nuclear membranes were decomposed, the tea polyphenols and proteins etc. were removed

    該法提取緩沖液使細胞維持一定的滲透壓，研磨時使細胞核基本保持完整；在細胞核被裂解之前去除細胞質中的茶多酚、大部分蛋白質和rna ；而後用sds裂解細胞核，異丙醇或乙醇沉澱dna ，這樣能經濟、快速和有效地從富含茶多酚、茶多糖等次生物質的茶樹新梢中提取基因組dna 。
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