親合層析 的英文怎麼說

中文拼音 [qīngěcéngxī]
親合層析 英文
affinity chromatography
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 層 : i 量詞1 （用於重疊、積累的東西 如樓層、階層、地層） storey; tier; stratum 2 （用於可以分項分步的...
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised, and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst

    Ptg誘導表達，超聲破碎細胞后，採用親和層析方法純化融合蛋白gst十nadc3 ，並以此為抗原免疫紐西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
  3. The synergy of the gravitational field with the other fields influences the mass transfer, heat transfer and affinity adsorption in the tapered bed, and the transfer directions depend upon the synergy results

    上流錐形床親和層析在多場協同下，其傳質、傳熱以及吸附反應相互影響，過程的最終傳遞方向將取決于各場之間綜合作用的結果。
  4. The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1

    因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。
  5. In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability, in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry, preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization

    本研究用改良后的親和層析法分離純化mr ， lowry法測定其蛋白質濃度，在精子穿透試驗（ spermpenetrationassay ， spa ）模型中定量研究其對精卵融合能力的影響並檢測其細胞生物學活性；以已知濃度的pmr （ purifiedmannose - ligandreceptor ）干預精子半透明帶試驗，觀察用pmr預處理半透明帶對精子與透明帶結合的影響。
  6. Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel

    融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease （ ppase ）酶切，酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后，得到純度較高的的m - centrin 。
  7. With ni2 + - lda - sepharose, the cbd tag was removed. according to the results of sds - page and western blot analysis, the product of outflow was the target protein lt a 27. the purity of lt a 27 was about 95 %

    運用niz +金屬鰲合親和層析柱進一步快速純化去除融合頭，上樣流出經sds一隊ge分析和western印跡分析ii1 :明得到的純化產物為淋巴毒素缺失體蛋白lt凸27 ， lt 2 :產物純度可達95 %以上。
  8. The recombinant protein exhibited specific binding to antiviral antibodies of h5 subtype and has no cross - reaction with h7 and h9 subtype

    分別用親和層析以及電洗脫兩種方法對gst - ha1融合蛋白進行了純化，都獲得了理想的純化結果。
  9. Fusion protein gst - hdt was purified by gstrap affinity chromatography

    將工程菌株發酵所得酶液，經gstrap親和層析，得到融合蛋白gst - hdt純品。
  10. Recently an abscisic acid binding protein with high specificity and affinity for aba was purified from epidermis of vicia faba l. in our laboratory, and further characterized using various biochemical techniques, there is evidence to link the protein to the physiological effects of aba in some aspects. the above - mentioned work may result in the molecular cloning the gene encoding aba binding protein

    近年來，我室利用親和層析從蠶豆下表皮中分離出一種高親合、高度特異性的aba結合蛋白，並完成了對其生化特性深入分析及部分測序，且在一定程度上揭示了該蛋白與aba的生理功能效應存在著內在的聯系，這為進一步克隆aba結合蛋白基因奠定了堅實的工作基礎。
  11. Then the product was purified by glutathione sepharose 4b chelation affinity chromatography, and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application

    表達產物經谷胱甘肽瓊脂糖4b親和層析純化后，得到了純度較高的gst - gnrh trs融合蛋白。為進一步科研和實際應用奠定基礎。
  12. Purification, identification of human telomerase and its protein subunits

    親和層析法分離鑒定人類端粒酶復合體及其蛋白質組分分析
  13. Purification and renaturation of recombinant human cu, zn - sod by metal - chelating affinity chromatography

    金屬螯合親和層析純化和復性重組人銅鋅超氧化物歧化酶
  14. After dialysis, the recombinant gst - ri was purified by rnase a - sepharose 4b affinity chromatography, and the single band of gst - ri was showed on a sds - electrophoresis gel to remove the extra urea

    復性的gst一班經knasea - sepharose4b親合層析純化， sds一page鑒定得到單一的gst一rj條帶。
  15. In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken

    為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。
  16. 2. purification copper binding protein using g - 25 column and copper chelating column. 3

    通過g 25層析和銅結合層析純化大鼠肝臟溶酶體中與銅具有親和性的蛋白質。
  17. The yeast recombinant expression vector pplc3. 5k - ndrg2 was constructed, then the ndrg2 protein was expressed in soluable form in pichia. after purification by metal chelate affinity chromatography, we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future

    為研究ndrgz的結構與功能，構建了ndrgz的酵母融合表達質粒，並在比赤酵母中得到可溶形式的表達；利用金屬螫合親合層析純化出了6his融合的ndrgz蛋白。
  18. Although hippocampal neurons and astrocytes also showed positive binding, cerebellar granule neurons are the more promising binding source because of their high yield, good binding and biological relevance. in the second part, prp was constructed into a recombinant protein with human igg fc fragment ( prpfc ) and used directly to interact with its bindin

    再次，在受體的鑒別和分離中，改進傳統的親和層析方法，以多功能交聯劑連接配體和受體，並分別以streptavidin及proteina偶聯的磁珠為結合反應載體，省lo以酉i
  19. Then it was subjected to hydroxylamine cleavage to verify the predicted primary structure. result : gst / mpem fusion protein was expressed successfully in e. coli. we obtained certain amount of gst / mpem protein by means of affinity chromatography

    結果：在大腸桿菌中誘導表達出gst mpem融合蛋白，經過親和層析獲得一定量的gst mpem融合蛋白，它們具有預期正確的一級結構。
  20. Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography

    方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。
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