親菌素 的英文怎麼說
中文拼音 [qīnjūnsù]
親菌素
英文
bacteriotropin-
Study of culture conditions for streptomyces
鏈霉親和素產生菌培養條件的研究The xerocomus spadiceus lectin, xsl, was isolated from extracts of fruiting bodies of the mushroom xerocomus spadiceus using a procedure that involved ( nh4 ) 2so4 precipitation, anion exchange chromatography on deae - cellulose, affinity chromatography on affi - gel blue gel, cation exchange chromatography on cm - cellulose, and gel filtration by fast protein liquid chromatography on superdex 75
從磚紅絨蓋牛肝菌（ xerocomusspadiceus ）子實體粗提物中，經過deae -纖維素陰離子交換層析、 affi - gelbluegel親和層析、 cm -纖維素陽離子交換層析和superdex75fplc凝膠過濾，純化了磚紅絨蓋牛肝菌凝集素。The resulting derivative strains produced antifungal activity in a manner different from the parental strain, indicating the original promoter was replaced with the engineered promoters and the epitopes
衍生菌株合成抗真菌抗生素的產量與親本菌株不同，表明fr - 008基因的原始啟動子已被改造后的啟動子和抗原決定簇所代替。The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8. vectors were amplificated in the e. coli dh5 a and were linearized with bgl ii. the linearized vctors were transformed into host strain gs115. the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa. the recombinated protein was detected with sds - page and western - blot as before
重組菌用甲醇誘導表達，用dot - elisa的方法篩選到表達量較高的菌株。將篩選出的菌株大量的誘導表達，對表達上清處理后，用sds - page和western - blot進行鑒定。同時，用hiprep16 10heparinff肝素親和柱對表達蛋白進行了初步的純化。First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。For neisseria meningitidis meningitis, household contacts and others who have had close personal contact with infected persons are recommended to receive a preventive antibiotic which kills bacteria living in nose and throat secretions
7 .曾與腦膜炎雙球菌病人有親密接觸的家庭成員和其他人士，應接受抗生素預防治療。This is an occasional cause of sore nipples, which can happen at any time after a period of trouble - free feeding. thrush can also occur after having been on antibiotics
真菌感染偶然也會引致乳頭損裂，多在正常授乳一段時間后才發生；也可能因母親曾服食抗生素藥物所引致。分享友人