誘導型啟動子 的英文怎麼說
中文拼音 [yòudǎoxíngqǐdòngzi]
誘導型啟動子
英文
inducible promoter-
As both the recombinant plasmids and the host dh5a had no laciq gene which encode lac represser, the trc promoter was not repressed by lac represser and always active. therefore the afpga could be constitutively expressed without iptg induction in the host dh5. in the shaking flask, the optimum ph and temperature for afpga expression was 7. 0 and 30
兩重組質粒再轉化宿主菌dh5 ,由於重組質粒和宿主菌dh5均不含laci ~ q調控基因,重組菌不產生阻遏蛋白,控製表達的trc啟動子始終處于「開」狀態,因而所得重組菌dh5 pkkfpga和dh5 psmlfpga不需誘導便能組成型表達pga 。We have created transgenic tobacco plants in which expression of the atnced3 coding region is under strong constitutive 35s cauliflower mosaic virus ( 35s ) promoter, stress - inducible rd29a promoter or stomotal - specific kst1 promoter by agrobacterium tumefaciens - mediated transformation
構建了組成型35s 、誘導型rd29a 、氣孔特異表達性kst1啟動子驅動atnced _ 3基因表達的真核表達載體,通過農桿菌介導的葉盤法轉化煙草,得到了轉基因植株。The characteristics of various cis - regulatory elements of plant promoters, such as tata - box, transcription start point and upstream elements, and different types of the plant promoter, such as constructive promoter, tissue - specific promoter and inducible promoter, as well as bi - direction promoter, alternative promoter and linked promoter will be described or analyzed in this present paper
並著重從組成型、組織特異型和誘導型啟動子3個方面介紹了其結構特徵、功能,以及它們在植物基因工程中的應用和研究進展,簡述了雙向啟動子、可變啟動子和串聯啟動子的研究情況,提出植物啟動子研究中存在的問題與展望。Gus expression induced by bth was systemic and had been detected in leaf, sepal, anther and mature pollen of transgenic tobacco plants, while induction effects of sa was spatial - temporally limited. 6
Bth能系統誘導pr - 1a野生型啟動子驅動gus基因在轉基因植株的葉、花藥、萼片及成熟花粉中表達,而sa的誘導作用則具有某種時空局限性。However, the mechanism for the later is not clear yet. our preliminary experiments suggested that extracellular calmodulin might be involved in rbcs gene expression in the dark. in order to provide direct evidence that extracellular calmodulin activated rbcs transcription in a light - independent manner, we intended to get the transgenic plants carrying light - dependent and - independent rbcs promoter fused to a reporter gene
如果細胞外鈣調素確實是光不依賴型rbcs基因暗中表達的誘導因子,它對其mrna水平的影響應是通過提高其轉錄水平即調節其啟動子活性實現的,我們計劃以轉番茄rbcs3a - gusa和rbcs3c - gusa的煙草懸浮培養細胞系為試驗材料進一步證實這一點。分享友人