誘導突變 的英文怎麼說
中文拼音 [yòudǎotūbiàn]
誘導突變
英文
induce mutation- 誘 : 動詞1. (誘導) guide; lead; induce 2. (使用手段引人隨從自己的意願) lure; seduce; entice
- 導 : 動詞1. (引導) lead; guide 2. (傳導) transmit; conduct 3. (開導) instruct; teach; give guidance to
- 突 : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
- 誘導 : guide; lead; induce; guidance; induction
- 突變 : 1 (突然急劇的變化) sudden change; change suddenly; transilience; accident; saltation; revulsion...
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Phenanthrene is one of three - aromatic pahs and distributes in environments widely, which causes diseases of human respiration system and skin, and induces mutation, aberrance and carcinogen of fish and other animals
菲為一種廣泛分佈於自然環境的三環芳烴,能導致人類呼吸系統和皮膚的病變,可誘發魚類和其它生物的突變、畸變與癌變。The ast ( anthocyanin spotted testa ) mutant, which was induced by carbon ion beam, was a single recessive gene mutant of arabidopsis thaliana and involved in the anthocyanin biosynthesis
擬南芥ast ( anthocyaninspottedtesta )突變體是由碳離子束誘導產生的與花青苷生物合成有關的突變體,受單隱性核基因控制。Expression of either dominant negative or antisense musk in cultured xenopus laveis spinal neurons abolishes agrin - induced neurite outgrowth inhibition and converts chemorepulsive growth cone turning induced by a gradient of agrin into chemoattraction
Musk突變體( dm - musk )或反義musk ( as - musk )的表達,解除了agrin對新生突起生長的抑制,並且把agrin誘導的排斥反應反轉為吸引反應; musk介導發育中神經元突起的生長。The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work
高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題,為基因的誘變找到了一條新途經。This paper introduces the principles and characteristics of magnaporthe grisea mutagenesis induced by physical and chemical inductions and genetic transformation briefly and reviews the research advances of these techniques
摘要系統地介紹了稻瘟病菌物理誘變、化學誘變和遺傳介導突變的原理、特點和研究進展,並評述了它們各自的優缺點。2. specific mutation from a to c at - 137 bp site upstream from transcription start point had no effect on the inducibility of the promoter in response to sa and bth. 3
在轉錄起始點上游137bp定點將a突變為c后,對化學啟動子的化學誘導應答性沒有明顯的影響,但對誘導效應的向上運輸存在一定程度的阻抑作用。900 bp promoter - directed gus expression was highly induced by sa and bth, while the 603 bp promoter, whether mutated or not, did not respond to sa and bth induction, which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site
全長900bp啟動子能夠應答sa和bth的誘導,而603bp長的啟動子無論突變與否對sa和bth均無應答,證明sa和bth的應答區域在克隆啟動子的轉錄起始位點上游575 872bp之間。The constructed vector was identified by sequencing. after induction by iptg, four polypeptides coding epitope expressed, respectively. research in this dissertation laid foundation for the development of nd - epitope - vaccine
經測序鑒定與原序列沒有突變及移碼后,經iptg誘導,有四段含表位的多肽獲得了表達。9 expression of mxmybl gene in e. coli was experimented. the structure of its recombinant expression vectors had not base mutant and drift. sds - page of pet - mxmybl proteins showed that there was a strengthened protein band in expectant 38kda protein marker
構建的原核表達載體結構正確,未出現堿基突變及移碼現象; lmmoffeiptg誘導zh后,在預期的蛋白分子量約38kda處出現一條表達加強的蛋白帶。Fig. 1 the pga22 phenotype. the pga22 mutant seedlings were grown in the absence ( far left ) or presence of increasing concentrations of estradiol ( second from the left to far right )
在逐漸增加雌激素誘導劑濃度后(從左側第二株至第六株) ,突變表型逐漸增強。For example, as a direct toxic effect, periodontal iruses and bacteria and their products ( endotoxins or enzymes, for example ) are toxic to surrounding cells and may directly induce mutations in tumor suppressor genes and proto - oncogenes or alter signaling pathways that affect cell proliferation or surial of epithelial cells
例如,作為直接毒性作用,牙周病毒和細菌及在細胞周圍其產生的毒性物質(例如,類毒素或酶類)可直接誘導腫瘤抑制基因和原癌基因突變或者改變細胞信號傳導通路從而影響上皮細胞增殖或存活。For example, as a direct toxic effect, periodontal viruses and bacteria and their products ( endotoxins or enzymes, for example ) are toxic to surrounding cells and may directly induce mutations in tumor suppressor genes and proto - oncogenes or alter signaling pathways that affect cell proliferation or survival of epithelial cells
例如,作為直接毒性作用,牙周病毒和細菌及在細胞周圍其產生的毒性物質(例如,類毒素或酶類)可直接誘導腫瘤抑制基因和原癌基因突變或者改變細胞信號傳導通路從而影響上皮細胞增殖或存活。( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1. 2 gene promoter. these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants. the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene, and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling
( 2 )對該啟動子的5 』端進行不同長度的缺失突變,突變的啟動子與gus構建的融合基因在煙草中受meja誘導的瞬時表達結果表明,該啟動子中- 300 - 243bp區域(有一個gccbox和一個gbox - likesequence )是其應答meja處理所必須的區域,並將- 300bp作為該啟動子應答ja信號的最小區域的邊界位置。( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter
( 3 )在缺失突變的基礎上,通過對gccbox及其相鄰的上下游六個堿基進行取代突變,將突變啟動子與gus構建融合基因,在煙草中受heja誘導的瞬時表達結果表明, h1和m3的突變對該啟動子應答ja信號的影響很小,而m2 ( gccbox的突變)則幾乎使該啟動子應答ja信號的功能完全喪失,所以gccbox是該啟動子中應答ja信號的必需元件。The annlic establishment gram clinical practice report has not borne the medicine phenomenon, also sudden change which to arab league past luo river wei gengxi luo river wei baijie induction and so on element produces is still sensitive
Annlic安立克臨床應用未報道耐藥現象,且對阿昔洛韋更昔洛韋白介素等誘導產生的突變株依然敏感。In this paper, an important cis - acting element within the promoter of pdf7. 2, which is activated by jas, was analyzed, and the interaction of this element with relevant factors ( jerfs ) was also studied. the results were as fellows : la ja responsive cis - acting element within the promoter of pdf 1. 2 was investigated via comprehensive mutant analysis. ( 1 ) a strategy based on cdna sequence was used to amplify the promoter ofpdf1. 2 gene with arabidopsis gdna as template
本文對受jas誘導的pdf1 . 2啟動子的順式作用元件及其與jerfs的相互作用進行了系統研究,取得如下結果: 1 、通過對pdf1 . 2啟動子的缺失突變分析和取代突變分析,對該啟動子應答jas信號的順式作用元件進行了較詳細的探討。Using this system, we have studied matrine - inhibittory effect and trifluoperation - neuroprotection effect in hippocampal slices, also discussed the mechanism of long - term potentiation using anesthetic rats. the experiment results showed that matrine can inhibit the hyperactivity induced by penicillin sodium in dosage by changing the relative parameters of field potential ; trifluoperation can alter ps change with the time, enhance the degree and the ratio of ps recovery, then minis the hypoxic injury ; high frequency stimulate can increase ps amplitude and epsp slope for long time, buildup the in / out function of nerve cells, and enhance synaptic plasticity
結果表明,苦參堿能夠劑量依賴性地抑制青霉素誘導的神經元順向信號傳導激活過程,使細胞外記錄到的場電位各個參數發生相應改變;三氟拉嗪可以改變ps的時相變化,提高ps的恢復程度和恢復率,減小了神經元因缺氧引起的不可逆損傷;高頻刺激( highfrequencystimulate , hfs )可以長時間的增強ps的幅度和epsp的斜率,進而增強神經元的輸入輸出功能,增加了突觸的可塑性。Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively
隨后,利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ,獲得了gx201的突變體菌株gx217 ;將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ,然後將pgxn201cl導入突變體菌株gx217 ,構建了gx217的功能互補菌株rgx217 。Some systems allow the irus to infect cells but do not permit prolonged replication and production of irus, while other systems rely on deriaties of permissie irus isolates for efficient replication in transformed ( mutated ) cell lines
一些培養體系可使病毒感染細胞而不能促進其大量復制繁殖,還有一些培養體系需要誘導劑使病毒在突變細胞株內充分復制。Whereas their parent mutant axr1 - 3 was male fertile, showed moderate insensitivity to ja - inhibitory root growth and mild reduction of ja - inducible gene expression ; parent mutant coi1 - 2 - was a leaky mutant allele of coi !, it exhibited reduced ja insensitivity and partial fertility. - taken together, all these results suggested that scfcoil complex was required for the ja response and that the axr1 - dependent rubl modification of atcull subunit of scfcol " was important for ja signaling in arabidopsis
試驗結果發現, coil 2 axrl 3雙突變體在根的生長、茉莉素誘導基因的表達和雄性育性方面對茉莉素都表現為完全抗性;而親本coil 2和axrl三突變體對茉莉素均表現部分不敏感性,表明axr基因和coil基因的同時突變協同增強擬南芥對萊莉素的不敏感性, axr基因與coi基因相互作用調控茉莉素的信號傳導途徑。分享友人