諸葛菜 的英文怎麼說
中文拼音 [zhūgěcài]
諸葛菜
英文
orychophragmus violaceus-
Two partially overlapping cdna fragments which were cloned from o. violaceus using the rt - pcr, 3 ' race ( rapid amplification of cdna ends ) and 5 ' race technique was assembled a 1758bp full cdna sequence of epsp synthase ( accession number in genbank : af440389 )
採用rt pcr技術與3 』 race和5 』 race的結合，以諸葛菜的總rna為反轉錄的模板，克隆、拼結出了諸葛菜的epsp合成酶的全長。 dna序列（該段cdan的genbank的登錄號為： af440389 ） 。The analysis of these two sequences and reported sequences ( three families, 15 sequences in total ) by nj method and the simplest principle shows, the evolutionary trees established by these two methods are consistent and the sequences of the same family constitute one group. the sequences of thaliana and the sequences of brassicaceae each makes up a sub - group in it. the sequences of violaceus distribute in brassicaceae sub - group and this phenomena shows that the relationship is very near
我們對這些序列與已發表的相應序列（合計3個科， 15個序列）用nj法和最簡約性分析，結果表明：由兩種方法構建的進化樹是一致的，同一科的序列形成一組:在十字花科組的分之圖中，來自於擬南芥屬的序列與來自於蕓墓屬的序列又各自形成亞組;來自於諸葛菜屬的序列分佈在蕓墓屬的分之圖中，揭示出諸葛菜屬與蕓矍屬有著極其近的親源關系。In vitro culture and plant regeneration of orychophragmus diffusus
鋪散諸葛菜的組織培養及植株再生A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。分享友人