豬疫白蛋白 的英文怎麼說
中文拼音 [zhūyìbáidànbái]
豬疫白蛋白
英文
suplagoalbumin-
Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method
鑒于eo蛋白在病毒感染,誘導機體免疫及與宿主細胞相互關系中的作用,本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析,揭示了我國豬瘟病毒流行株之間的遺傳演化關系,為豬瘟病毒的流行病學研究提供依據。Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。Using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. it was found that antigens sp - 10 and aeg express on acrosome of all kinds sperms, supporting their roles in sperm - egg fusion. it was firstly described that tmdc - iii express mostly on the equator, neck and some part of tail of human sperm, on the acrosome of mouse and bull sperms, on the acrosome and neck of pig sperm, on the head, neck, and middle piece, principal piece of tail of rabbit sperm
利用間接免疫熒光法對aeg 、 sp - 10 、 tmdc -蛋白在人、小鼠、兔子、豬、牛精子上進行了精確定位, aeg 、 sp - 10在頂體部均有表達,確證了它們與精卵融合有關:首次對tmdc -蛋白在人、小鼠、兔子、豬、牛精子進行了精確定位,它主要存在於人精子尾部主段,小鼠和牛精子頂體,豬精子頂體和頸部,兔精子頭部、頸部和尾部的主段及尾段。In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit
本室利用e2基因疫苗制備了多株單抗,為e2的抗原表位研究提供了條件,我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位,為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。6 weaning pigs were immunized with membrane protein, and their antibodies were 1 : 23. 50 ( immunized after 7 days ), 1 : 26. 33 ( immunized after 14 days ) and 1 : 27. 83 ( immunized after 21 days ). humoral and cellular immunity tests testified immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein was good
從細胞免疫和體液免疫試驗的結果可以看出,豬肺炎支原體膜蛋白具有良好的免疫原性,能誘導動物機體產生較強的細胞免疫和體液免疫應答反應。These essential component, hemagglutination, toxicity, immunogenicity of mycoplasma hyopneumoniae strain xy - 1 membrane protein were studied. the range of membrane protein mw is from 14kd to 100kd by means of sodium dodecyl sulfate - polyacrylamide gel electrophoresis, and molecular weights of the protective antigens were 36kd and 64kd by sds - page
本研究以分離豬肺炎支原體野毒株( xy - 1株)的膜蛋白為試驗材料,系統地研究了膜蛋白的分子量范圍、血凝性、毒性及免疫原性等。The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza
結果表明所獲得的重組病毒( rprvha )遺傳性狀穩定,在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白,為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria. the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit, and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128, 000
從制備性聚丙烯酰胺凝膠中回收融合蛋白,用mtt法測定重組蛋白刺激小鼠脾淋巴母細胞增殖反應活性,並以重組蛋白免疫家兔制備抗豬il - 6滴度為128 , 000的高免兔血清。Classical swine fever ( csf ) is one of major contagious diseases in pig breeding industry. in order to gain massive diagnostic antigen csfv, and large scale of csfv specific major protective antigen protein ( e2 envelope glycoprotein ) in eucaryotic cells, which are prepared into subunit vaccine for effective prevention against csf
豬瘟是危害養豬業的主要傳染病,為了得到大量的豬瘟病毒特異性診斷抗原,並試圖在真核細胞中大量表達豬瘟病毒特有的免疫保護性抗原,即e2囊膜糖蛋白,為進一步制備豬瘟亞單位疫苗有效預防本病奠定基礎。The rabbits were haintmed with purified recombinant protein and the sera were examined for anti - tgev neutralwi astivity on the continuous porcine ibrs2 cells. the result showed that neutraldrig activity was 1 : 22. therefore, the neuthelizing activity of anti n protein antibody against infectious tgev in vitro was very iow from the result of neutra1hation ( vn ) test
將純化的融合蛋白免疫家兔,制備的抗血清在豬ibrs _ 2傳代細胞上進行中和實驗,結果顯示中和效價為1 : 22 ,表明n蛋白所產生的抗體對tgev的中和活性較低。分享友人