質子受體 的英文怎麼說
中文拼音 [zhízishòutǐ]
質子受體
英文
proton acceptor-
First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先,用~ ( 35 ) s標記的氨基酸混合物喂養工程菌成功地制備了~ ( 35 ) s標記的擬南芥鈣調素( ~ ( 35 ) s - cam ) , ~ ( 35 ) s - cam純度高、放射活度高、 ca ~ ( 2 + )與egta存在時的電泳行為與未標記cam相同,可作為一種高靈敏性的探針用於進行受體學分析實驗;用擬南芥種子誘導愈傷,通過酶解制備了大量原生質體。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。This ion channel, present in the membrane of many nociceptors, responds not only to capsaicin, the pungent ingredient in chili peppers, but also to distressful heat and to protons ( the hydrogen ions that make substances acidic ) ; protons are unusually abundant in inflamed tissue
該受體是一種離子通道,出現在許多痛覺受器的細胞膜上,不只對辣椒的辛辣成份起反應,也對傷人的熱及質子(造成酸性的氫離子,在發炎組織中特別多)起反應。Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物,對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明,蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用,也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中,凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物,對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明,蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用,也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中,凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Vascular endothelial growth factor and its receptor in brain glioma
血管內皮生長因子及其受體與膠質瘤Expression of vascular endothelial growth factor receptors in gliomas
血管內皮生長因子受體在膠質瘤中的表達According to medical research, when vitamin b12 enters the body, it forms a compound with an intrinsic factor secreted by the parietal cells large cells of the peptic glands on the gastric mucosa mucous membrane of the stomach, before being absorbed by receptors in the ileum lower part of the small intestine in the presence of calcium ions
在維他命b12的吸收方面,根據醫學報導,維他命b12在吃進人體后,首先會在胃部,和胃壁細胞parietal cells分泌的一種蛋白質內因子intrinsic factor結合,形成復合物后,再由小腸段中的回腸ileum吸收。在回腸之接受體receptors吸收時,需要有鈣離子之存在。Using the young seedlings of wild type ( wt ) and four mutants ( phya, phyb, phyaphyb and hy4 ) of arabidopsis thaliana as plant materials, the effects of light quality and kinetin ( kt ) on some photomorphogenesis responses such as cotyledon enlargement, hypocotyl growth inhibition, accumulation of chlorophyll and anthocyanin were investigated in the present study. photoreceptor functions and interaction of light and kt in the responses was examined and discussed
本文以擬南芥( arabidopsisthaliana )野生型( wt )和四種光突變體phya 、 phyb 、 phyaphyb和砂hy4為材料研究不同光質與細胞分裂素( kt )對幼苗子葉擴大、下胚軸伸長、葉綠素和花色素苷積累的影響,分析光受體參與的反應以及探討光與kt在調節各種反應中的相互關系。Ip3 - ip3 receptor ( ip3r ) interaction mediates the release of ca2 + from the endoplasmic reticulum in response to many different extracellular stimulus. for higher plants, however, though it is now generally accepted that ip3 participates in signal transduction in many important cellular processes, only limited evidence is available for the presence and properties of the ip3r - like protein so far. here, using the immunological methods with an antibody raised against a mammalian inositol 1, 4, 5 - triphophate receptor ( type 1 ), we found that, 1 ) the antibody across - reacted the proteins with about 200kd in microsomes from oryza sativa and about 200kd from arabidopsis thaliana respectively
本實驗用sds - page電泳和免疫印跡的方法,用哺乳動物大鼠三磷酸肌醇受體的多肽做抗體對類三磷酸肌醇受體蛋白鑒定,結果表明:抗體與水稻和擬南芥微粒體蛋白分子量大約為200kd的蛋白交叉反應,同時還發現在水稻微粒體蛋白62kd和擬南芥微粒體蛋白45kd處有交叉反應的蛋白條帶存在,表明在植物中有類三磷酸肌醇受體蛋白的存在;用免疫膠體金方法,發現類三磷酸肌醇受體蛋白主要分佈於液泡膜和細胞質膜上。Xanthophyli cycle has been widely concerned since the finding that it has the function in heat dissipation. with the trans - thylakoid ph gradient, zeaxanthin ( z ) together with antheraxanthin ( a ) can absorb excess energy from chlorophyll and release it as heat, thus protect the photosynthetic apparatus from photodamage by high light. violaxathin de - epoxidase ( vde ) is the key enzyme in xanthophylls cycle
目前普遍認為葉黃素循環的色素定位於天線色素蛋白復合體上,在跨膜質子梯度( ph )形成后,玉米黃質( z )和環氧玉米黃質( a )能夠從葉綠素中吸收過多的激發能,並以熱能的形式耗散到體外,從而保護光合器官免受強光的破壞。Significance of expression of vegf - c and vegfr - 3 in different histopathological grade of astrocytoma
人腦膠質瘤血管內皮細胞生長因子受體表達的研究Objective the present study was to investigate the effects of endotoxin on capacitation and acrosome reaction ( ar ) of mouse, golden hamster and human sperm, on the sperm - oocyte fusion in mouse, and on the development of 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos in vitro. the purpose was to definite the committed step and the mechanism during in vitro fertilization ( ivf ) on which endotoxin affected, and to distinguish the sensitivities to endotoxin of three developmental systems of mouse embryos. all these data would provide the reference to clinical and laboratory quality control
目的研究內毒素對小鼠、金黃地鼠及人精子的體外獲能和頂體反應、小鼠精卵結合及小鼠1 -細胞、 2 -細胞和去卵透明帶2 -細胞胚胎體外發育的影響,探討內毒素影響體外受精結局的環節及可能的機制,確定三個體外培養系統對內毒素的敏感性,以期為臨床和實驗室質量控制提供參考。Layered and pillared material are a kind of multifunctional material which were developed in recent years, much attention has been paid to this kind of material for its application in ion - exchange catalysts solid state proton conductivity, nonlinear optics and physic. a lot of literature have reported the intercalation behavior of a - zirconium phosphate ( abbreviated as a - zrp ), different guest molecules inserted into a - zrp have been studied in detail, those guest molecules include amine, alcohok amino acid protein, enzyme coornadiate compound and coronal compound. the intercalation guest is restricted by their size and basicity
層柱材料是近年來發展起來的一類多功能材料,由於其在離子交換、催化、固態質子導體、非線性光學以及醫學等方面的廣泛應用而受到國內外研究者的重視,大量文獻報道了-磷酸氫鋯zr ( hpo _ 4 ) _ 2 ? h _ 2o ( - zirconiumphosphate ,縮寫為- zrp )的超分子插層化合物及插層性能,其中對不同的客體分子對磷酸鋯的嵌入做了詳細的報道,客體分子的種類包括氨、醇、氨基酸、蛋白質、酶、配合物、冠狀化合物等。Rubber tree ( hevea brasiliensis ) is an important economic woody - crop in tropical areas. its latex is the unique source of crude rubber used in current industry. because of its special and important use, the rubber tree has been extensively planted in tropical areas. increase production is always the main target in rubber tree cultivation. since the ethrel was applied in increasing latex production in 1968 for the first time as a chemical stimulant, not only the latex production had been increased largely, but also a new set of rubber tapping system had been established, leading to a series of economic benefit. owing to ethrel " s extensive application, its side effects had been found more and more obviously, such as tapping dry, speeding up senescence, shortening the life span of rubber tree etc. in order to overcome the side effects and increase production more availably, for a long time, people had carried out lots of research work on cell level, membrane level, physiology and biochemistry of laticifer contents. but the mech anism why ethrel increased latex production was not yet understood completely. this study had cloned the ethylene receptor gene ( efrl ) from rubber tree, and researched the relationship between etrl expression in laticifers and ethrel stimulation on transcription level and protein translation level. the results were as follows : 1
但是,由於乙烯利應用的普及,乙烯利刺激割膠引起橡膠樹發生死皮病及加速膠樹衰老,縮短膠樹壽命等副作用也越來越明顯。為了克服這些副作用,使乙烯利能更有效地刺激增產,長期以來,人們在細胞水平、膜水平和乳管細胞內含物的生理生化層面上進行了大量的研究,但仍未完全了解乙烯對膠樹的作用機制。本研究從分子水平入手,克隆橡膠樹的乙烯受體基因( etr1 ) ,並在轉錄水平和蛋白質翻譯水平上研究etr1基因在乳管細胞中的表達及與乙烯刺激的關系,取得了以下結果: 1It is necessary to study aerobic treatment with in - situ bioremediation by means of inoculated microorganism and added nutrition and co - metabolism substrate
接種微生物、添加電子受體、營養鹽和共代謝基質是生物修復的主要技術手段。The mrd, firstly discovered by us with neuroanatomical and immunohistochemical methods, was a flat, pan - shaped structure at the caudomedial margin of the neostriatum and surrounding the rostrolateral edge of the globus pallidus in brains of the rat [ 12 ], cat [ 12 ], monkey [ 13 ] and human
研究的目的及意義:谷氨酸是哺乳類動物中樞神經系統的一種興奮性神經遞質,其受體分為離子型和代謝型兩類。離子型具有完整的陽離子通道,而代謝型( mglut )則通過蛋白轉到胞內信號系統而起作用。The neurotrophic and morphogenic activities of gdnf are mediated by its interaction with a multicomponent receptor complex formed by the ret receptor tyrosine kinase and a glycosylphosphatidylinositol ( gpi ) - anchored " accessory " receptor, gdnf family receptor alpha - 1 ( gfral )
Gdnf的神經營養作用主要是通過兩類受體亞基介導,即膠質細胞源性神經營養因子受體alpha ( gfr s )亞基和受體酪氨酸激酶ret亞基。Differentiation of 3t3 - l1 preadipocytes into adipocytes is induced by a combination of inducers, including a glucocorticoid, an agent that elevates cellular camp, and a ligand of the insulin - like growth factor - 1 receptor
3t3 - l1前脂肪細胞是在三種誘導劑(一種糖皮質激素,一種能夠提升胞內camp水平的試劑和一種類胰島素生長因子受體的配體)的誘導下分化成為脂肪細胞。分享友人