質粒體分析 的英文怎麼說

中文拼音 [zhílìtǐfēnxī]
質粒體分析 英文
plasmid analysis
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 粒 : Ⅰ名 （小圓珠形或小碎塊形物） small particles; grain; granule; pellet Ⅱ量詞（用於粒狀物）
  • 體 : 體構詞成分。
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  • 析 : Ⅰ動詞1. （分開; 散開） divide; separate 2. （分析） analyse; dissect; resolve Ⅱ名詞（姓氏） a surname
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. The structures and characteristics of several graphite samples are measured by means of powder x - ray diffraction ( xrd ), brunauer - emmer - teller ( bet ) surface area measurement, inductively coupled plasma ( icp ) spectroscopy, particle size analysis and electrochemical measurements. the effects of origin, structure, impurity, particle size, specific surface area of carbon materials on the electrochemical characteristics are studied. a synthetic graphite with abundant resources, low cost and favorable performance is determined as the raw material for modification of graphite

    採用xrd 、 bet 、 icp 、激光粒徑分析及電化學性能測試等方法，對國內外多種典型石墨樣品的結構與性能進行比較，研究石墨材料的來源、晶體結構、雜質含量、顆粒大小、比表面積等因素對其充放電性能的影響，確定一種性能較好、價格低廉、來源廣泛的普通人造石墨粉作為熱處理與摻雜改性、以及復合結構炭材料研究的原材料。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. From sedimentology symbol such as rock assembled, sediment gradus, sandstone grading analysis, protogene sedimentary structure, ichnofossile and logging facies etc and palaeoenvironment background, it is confirmed that shen84 - an12block s34 segment is braid river delta system, not fan delta system ( abrupt slope ). then it can be more partition as braid river delta plain sedimentary assembled, delta front sedimentary assembled and prodelta sedimentary assembled on core and log assembled. and delta plain sedimentary assembled can be identified four microfacies : braided distributary channel, crevasse splay, overbank deposits and interdistributary bay

    沉積微相研究是儲層非均質性研究的基礎，研究區有豐富的巖芯資料和測井資料，通過巖石組合、沉積韻律，砂巖粒度分析、沉積構造、古生物遺跡和測井相等沉積學標志，結合古環境背景，確定沈84 ?安12塊s _ 3 ~ 4段為辮狀河三角洲體系，而不是前人所認為的扇三角洲（陡坡型）體系，在巖芯和測井組合上可進一步劃分為辮狀河三角洲平原沉積組合、三角洲前緣沉積組合和前三角洲沉積組合，其中三角洲平原沉積組合又可識別出辮狀分流河道、決口扇、分流河道邊部和分流間灣四種微相類型；三角洲前緣沉積組合識別出水下分流河道、水下分流河道邊部和分流河口壩三種微相。
  5. The highest antiviral litre of rpoifn - a was l l08iu / mi. the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro

    Sds - page分析表明，構建的重組表達質粒pqe30 poifn在大腸桿菌jm109中表達的rpoifn占菌體蛋白總量的20 26 。
  6. Many research such as clay minerals, suspend in the sea, the grain size trend analysis of surface sediment, poc 6 cu and 8 c13 in bottom sediments etc. showed that : the yellow sea warm current divided the south yellow sea into two, the terrestrial material of modern sediment on the west shelf plain mainly came from the modem yellow river suspend carried by the alongshore current, and the sediment difference between the west and east side of yellow sea trough was represented by the sedimentation thickness in postglacial period, sedimentation stratum, and oxygen isotope record

    粘土礦物、碳酸鹽、粗碎屑、海域懸浮體、表層沉積物粒度趨勢分析、 poc c ~ （ 13 ）及底質沉積物的c ~ （ 13 ）等多方面研究表明：以黃海暖流為主導，南黃海堆積陸架平原西側現代沉積物陸源物質主要源於黃海沿岸流攜帶的現代黃河懸移物質，黃海槽東西兩側的沉積差異主要表現在冰消期以來沉積厚度、沉積層序、氧同位素記錄上。
  7. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  8. The hydraulic gradients of settling slurry flow transported in three kinds of moving states, i. e. suspension, saltation, or partially suspension were investigated, based on analyzing the changes of solid particles and water in momentum, velocity and their mass related to momentum exchanging over the acceleration period of the solid particles

    從固體顆粒加速期間清水與固體顆粒的速度變化、動量傳遞、相關質量等基本問題分析入手，研究了沉降性漿體在水平管道內流動時其固體顆粒在3種流動狀態下的水力坡度，提出了沉降性漿體在水平管道內流動的機理模型，進而用該模型對一些輸送條件下的沉降性漿體的水力坡度進行了計算。
  9. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  10. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  11. The centre of the dark sector soliton evolution equation is derived by means of particle analytical method, equivalent form dark soliton ' s evolution equation in the photorefractive crystal, esceptialy between tow dark soliton ; the potential expression and it ' s numerically calculation is achieved, the conclusion is drawn : when the distance between two dark soliton = 0, its potential function has minimum, shows there is an stead soliton in the photorefractive maternal, and interaction between two dark soliton raches biggest ; when the distance between two dark soliton 5, its potential function is nearly equal zero, shows that the interaction between two lark soliton is very weak, nearly zero

    摘要本文從光折變晶體中能得出暗孤子所滿足的演化方程出發，用粒子分析力法得出了兩暗孤子相互作用時，其質心間的距離所滿足的演化方程，並得出了兩暗孤子相互作用勢函數的表達式，得到其數值解，並得出如下結論：當兩暗孤子間距離= 0時，其相互作用勢函數有最小值，表示在光折變晶體中有穩定的暗孤子對，並且相互作用達到最大；當兩暗孤子距離5時，其相互作用勢函數幾乎為零，表示兩暗孤子間相互作用很弱，幾乎為零。
  12. This paper conbined with the indoor test and the scene test road, through the synthetical analysis of the factors of influencing densification effect for the densification thickness of filling stone roadbed. the maximum grain size of fill material, densification machinery and densification frequency et al. advanced a control norm of the maximum grain size and piy of compression, established ration evaluating the control standard of densification quality of filling stone roadbed under overload. comparing with result of indoor test and the scene test road, studied on theory of densification in different kinds of stone material and perfected the control system of the densification of filling stone roadbed under overload

    結合室內試驗和現場試驗路的實測數據，通過對填石路基的壓實厚度，填料最大粒徑，壓實機械及壓實遍數等影響壓實效果的因素的綜合分析，提出填石路基的最大粒徑和攤鋪厚度的控制指標，建立定量評定超重載交通下填石路基壓實質量的控制標準，並對照室內試驗和現場試驗結果，研究不同石料條件下的壓實理論，補充完善了填石路基壓實控制指標體系。
  13. Libs has power potential to be used to detect trace elements in the sample matrix that include gases, aerosols, and liquids

    因此libs可運用於氣體、顆粒和液體基質中的痕量分析。
  14. In 2005, 61 physical and chemical parameters were measured and analysed in sediments. these included particle size, electrochemical potential as highly anoxic sediment with negative potential is related to organic pollution, chemical oxygen demand which indicates organic pollutants, total sulphide inorganic constituents, source of the unpleasant - smelling gas hydrogen sulphide, 15 metals and metalloids aluminium, arsenic, barium, boron, cadmium, chromium, copper, iron, lead, manganese, mercury, nickel, silver, vanadium and zinc, and trace toxic organics pahs and pcbs - 16 compounds and 18 congeners respectively

    2005年，沉積物監測包括分析61種物理及化學參數，其中有粒子大小電化學勢有機污染促使沉積物缺氧，而讓電化學勢呈負數值化學需氧量顯示有機污染程度總硫化物無機物，是造成難聞氣體硫化氫的主因15種金屬及準金屬鋁砷鋇硼鎘鉻銅鐵鉛錳汞鎳銀釩及鋅和痕量毒性有機物多環芳烴及多氯聯苯分別為16種復合物及18種同質物。
  15. Several new physics experiments in 1998 were performed and analyzed to showthe subtlety of quantum theory, including the “ wave - particle duality ” and the nonseparability of two - particle entangled s tate. here it is shown that the measurement is bound to change the object by dest roying the original quantum coherence between the object and its environment. so the “ physical reality ” should be defined at two levels, the “ thing in itself ” and the “ thing for us ”. the wave function in quantum mechanics is just playing the role for connecting the two levels of matter via the fictitious measurement

    在1998年完成和分析的幾個新的物理實驗顯示了量子理論的微妙性，包括「波粒二重性」以及二粒子纏結態的不可分性.本文的分析表明：測量在破壞原來存在於客體及其環境間的量子相干性時必然要改變客體.因而「物理實在」應在兩個層次上定義： 「自在之物」與「為我之物」 .量子力學中的波函數則正起了通過「虛擬的測量」將這兩個層次的物質聯系起來的作用
  16. Abstract : several new physics experiments in 1998 were performed and analyzed to showthe subtlety of quantum theory, including the “ wave - particle duality ” and the nonseparability of two - particle entangled s tate. here it is shown that the measurement is bound to change the object by dest roying the original quantum coherence between the object and its environment. so the “ physical reality ” should be defined at two levels, the “ thing in itself ” and the “ thing for us ”. the wave function in quantum mechanics is just playing the role for connecting the two levels of matter via the fictitious measurement

    文摘:在1998年完成和分析的幾個新的物理實驗顯示了量子理論的微妙性，包括「波粒二重性」以及二粒子纏結態的不可分性.本文的分析表明：測量在破壞原來存在於客體及其環境間的量子相干性時必然要改變客體.因而「物理實在」應在兩個層次上定義： 「自在之物」與「為我之物」 .量子力學中的波函數則正起了通過「虛擬的測量」將這兩個層次的物質聯系起來的作用
  17. Based on the experiment of dust removal in a granular layer at normal temperature and the analysis of the experimental results, the influence of filter particle diameter, layer thickness and filtration velocity on the dust removal efficiency was researched

    摘要在常溫下，採用固體顆粒層進行過濾除塵的實驗研究，並對實驗結果進行分析，探索了過濾介質粒徑、過濾層厚度和過濾速度對除塵效率和床層壓力差的影響。
  18. Abstract : the authors discuss roundly source of loose earth fo r impact - deposit debris flow to develop taking pingchuan debris flow, one of the quite typical impact - deposit debris flows in southwest sichuan province ， as an example in this paper. on the basis of detailed investigations in - situ, geotechn ial testing in lab and theory analysis, all results indicate obviously that moder ate and bulky loose earth in giant debris flow situating in southwest sichuan mo untainous area are main institute, whose tribulation are consistent with landform s. loose earth locating in mid - forepart catchment are quite unstable, so often be comes main source of loose earth of debris flow. formation of loose earth is cont rolled principally by geologic and geotechnical environment while human actions is the secondary factor for loose earth to form. in many times, human actions prov ide loose earth through loose earth stability

    文摘:本文以極具典型的平川泥石流為例比較全面的分析了沖淤變動型泥石流的物源問題.根據現場調研及室內巖土分析進行的研究成果顯示，川西南高山深谷地區的大型泥石流溝中的鬆散土體以中、粗粒段為主，其分佈與地形密切相關；位於泥石流溝中、前部位的鬆散土體的穩定性最差，是補給泥石流的重要物源；鬆散土體的發育主要受控于地質及巖土環境，人類活動只能影響鬆散土體的穩定性
  19. Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area, in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china, thus providing theoretic basis for the study of vaccine against it. the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages. when the cpe was obvious, the virus was harvested and purified

    為了弄清我國不同地區prrsv的來源以及其遺傳學背景，為疫苗學研究提供理論根據，本研究在ch - 1a株完整的基因組獲得以後，從流行於我國山東（ sd ）和黑龍江大慶（ dq ）地區疑似prrs的豬體內分離到prrsv ，在mark - 145細胞上盲傳5 6代，細胞出現明顯病變以後，收獲病毒液，然後提純，提取全病毒rna ，經過反轉錄、 pcr擴增獲得結構基因orf2 7的目的基因片斷，然後與pmd - t載體連接，轉化，得到陽性質粒后進行測序，並將其與ch - 1a株進行了比較分析，同時對這兩個毒株的結構基因組的理化性質進行分析。
  20. The properties of these doped powders, the microstructure and composition of these rare - earth co - doped tungsten matrices and cathodes have been investigated by size analysis, xrd, sem and edax. the electronic emission performances of these cathodes are measured in uhv electron emission surveyor. aes is adopted to analyze the atom composition and diffusion behavior of active elements on cathode surfaces

    通過粒度分析、 xrd 、 sem 、 edax研究了摻雜粉末的特性、燒結基體和陰極的微觀結構和成分;用動態真空電子發射測試儀對上述陰極進行了電子發射水平的測試;採用aes對陰極表面原子組成和活性物質的擴散行為進行了研究，分析了陰極發射水平與表面原子組成的關系。
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