質體原基 的英文怎麼說
中文拼音 [zhítǐyuánjī]
質體原基
英文
plastid primordia-
This paper introduced the application of biotechnology in rice genetics and breeding, including tissue culture, cell mutants selection, protoplast fusion, apomixis, molecular mark assisted breeding and genic transformation
簡要綜述了生物技術在水稻遺傳育種中的應用,主要包括組織培養、細胞突變體的篩選、原生質體融合、無融合生殖以及分子標記輔助育種和轉基因技術等方面。Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先,用~ ( 35 ) s標記的氨基酸混合物喂養工程菌成功地制備了~ ( 35 ) s標記的擬南芥鈣調素( ~ ( 35 ) s - cam ) , ~ ( 35 ) s - cam純度高、放射活度高、 ca ~ ( 2 + )與egta存在時的電泳行為與未標記cam相同,可作為一種高靈敏性的探針用於進行受體學分析實驗;用擬南芥種子誘導愈傷,通過酶解制備了大量原生質體。A mixture of catechin and epicatechin, procyanidin b4, 4 - hydroxy - benzoic acid and ethyl gal - late were purified and identified from grape seed procyanidin extract
得到2種原花青素的單體: ( + ) -兒茶素和( - ) -表兒茶素; 1種原花青素二聚體b4 ; 2種其他酚類物質:對羥基苯甲酸及沒食子酸乙酯。Prokaryotes lack organelles such as nucleoli, mitochondria, plastids, golgi apparatus, and do not exhibit cyclosis
原核生物無細胞器,如核仁、線粒體、質體、高爾基體等,不出現胞質環流。Here we studied the relationship of various factors and the quality of protoplasts. which maybe could be the basic of moss gene targeting. results showed : inoculated the spores onto diferrent kinds of media, such as ms, benecke and knop, we found that there was no difference when the spores germinated and differentiated into cauliform soon
通過對立碗蘚的無菌培養和原生質體操作發現: ( 1 )立碗蘚孢朔接種在無菌ms 、 benecke 、 knop培養基上,均可萌發產生原絲體,但不久便分化為莖葉體,很難長期保持其原絲體狀態,不同培養基條件下原絲體狀態有所不同。Ground state energy of a pollution state in polyatomic polar crystals
多原子極性晶體系統中雜質態的基態能量And the viviparous plantlets of both species are formed for the differentiation of floral primodium, but the position if viviparous plantlets is different. for the species of polygonum viviparum l., the viviparous plantlets are formed in the inflorences, while in saxifraga cernua l are in the leaf axils. on the results of observation for morphology and anatomy, vascular bundles of viviparous plantlet are connected with that of their parent
從來源和性質看,二者的胎生苗均由花原基直接分化形成;但從位置上看,珠芽蓼在花序中形成胎生苗,而珍珠虎耳草則在地上莖的葉腋部位形成胎生苗;從形態解剖角度觀察,胎生苗的維管束與母體的維管束直接相連,胎生苗脫落時,其上已長出葉片1 - 3枚。In recent years, the carst collapses that are occurring frequently in qixiashan hill area severely endanger the engineer and human life. there are many factors caused the occurring of the carst collapse in this area. besides the background of geology and geomorphology, the causes of changing of hydrodynamic, stress field of the earth s crust, and structure of rock and ground are more important
近年來,南京棲霞山地區巖溶地面塌陷頻繁發生,造成了巨大的經濟損失。巖溶地面塌陷的產生,除了地質地貌基礎外,更主要原因在於礦山開采改變了水動力條件地應力場及巖土體結構,尤其是礦坑疏干對水動力條件的影響最為顯著。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。" for the achievement of bose - einstein condensation in dilute gases of alkali atoms, and for early fundamental studies of the properties of the condensates
在「堿性原子稀薄氣體的玻色愛因斯坦凝聚態」以及「凝聚態物質性質早期基礎性研究」方面取得成就First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后,考察了影響兩親株原生質體形成和再生的主要因素,確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件:前者用s培養基,在28 、 220r . min ~ ( - 1 )培養24h后,用3mg ml的溶菌酶在32恆溫酶解50 60min ,得到的原生質體在乾燥的r5培養基上28倒置培養5 6天,可得到再生率在20左右的再生菌落;後者採用二級菌絲培養,用1mg ml的溶菌酶在37恆溫酶解1h左右,得到的原生質體也在乾燥的r5培養基上28倒置培養5天,即可得到再生率在20左右的再生菌。But in s. mycarofaciens, there is no distinctive propionate kinase, so that the utilization rate of propionic acid is lower than that of acetic acid. for this reason, it is hoped that the higher producers would be obtained by protoplast interspecies fusion between s. mycarofaciens and s. erythreus, which would transfer propionate kinase of s. erythreus to s. mycarofaciens. therefore, the fusion cells could use propionic acid as precursor in synthesis of mdma _ ( 1 ), which would reduce the production costs
根據文獻報道,紅黴素鏈黴菌中的丙酸激酶對丙酸的利用率是對乙酸的利用率的13倍;而在生米卡鏈黴菌中,無特異的丙酸激酶,菌體對丙酸的利用低於對乙酸的利用,因此希望利用原生質體種間融合的方法將紅黴素鏈黴菌的丙酸激酶基因轉移到生米卡鏈黴菌中,從而使融合子能夠利用丙酸鹽作為合成mdma _ 1的前體,提高mdma _ 1的產量,降低生產成本。This paper analyzes the percolation mechanism and characteristics of gas under low - velocity percolation state in terms of slippage effect and threshold pressure effect by using molecular dynamics, thermodynamics and percolation mechanics in order to understand the practical reason of low - velocity non - darcy percolation
摘要為明確氣體在低速滲流狀態下的滲流規律以及產生低速非達西滲流的實質性原因,運用分子動力學、熱力學和滲流力學等相關知識,基於滑脫效應和閾壓效應兩方面分析了氣體在低速滲流狀態下的滲流機理及滲流特徵。The results showed that it contains akaloids, phenols, organic acids, reducing sugars and polysaccharides, saponins, sterols and amino acids, but not tannins and protein
結果表明,多裂駱駝蓬水浸液含有生物堿、酚類、有機酸、還原糖和多糖類、皂貳、街體、氨基酸,不含蛋白質和鞣質。A typical bistatic weather radar net work is comprised of one traditional transmitter - receiver radar and one or more passive non - transmitting radar receiver at remote site. this type of multiple radar network offers unique advantages when compared to traditional radar net work. this paper introduces the concepts and principles of bistatic weather radar network and analyses, simulates, calculates the basic concepts of effective scattering particle volume relative to the receiver of bistatic radar network, the doppler shifts of the received signals, gives the formula to conduct the three dimensions wind field
本文從原理上分析了雙基地天氣多普勒雷達的構建、基本幾何關系、有效散射體積及其與雙基地角的關系,有效散射體積和目標與雙基地天氣多普勒雷達主站發射機的距離的變化關系,指出雙基地天氣多普勒雷達有效體積和單基地雷達有效照射體積隨之距離的變化有相似之處,但是由於收發分置的幾何結構,雙基地天氣多普勒雷達的性質與雙基地大氣多普勒雷達的散射角有著密切的關系。The results obtained in our laboratory in the past decade years showed, apoplast calmodulin in plant kingdom may regulate a lot of growth and development process of plant, such as accelerating the proliferation of angelica dahurica suspension cells and the proplast cell regeneration, startup the pollen germination of many plants " pollen and accelerating the elongation of the pollen tube, stimulating the redox of corn root " s cell, inducing the expression of light independent rbss gene, and participating in the regulation of the restraining function of al ~ ( 3 + ) to pollen tube germination
我室多年的研究結果表明,植物質外體cam可能做為多肽第一信使調節著植物體諸多的生長發育過程:如促進白芷懸浮細胞的細胞增殖和原生質體壁再生,啟動並促進多種花粉的萌發和花粉管的伸長,刺激玉米根細胞的氧化還原反應,誘導rbcs基因光不依賴的表達,以及參與調節胞外al ~ ( 3 + )對花粉萌發的抑制效應等。Extro growth hormone releasing factor mediated by protoplast could more than 28 days retention in rabbit and mouse muscle that is condition of long - time expression of grf
通過提取注射部位dna后進行pcr鑒定,可以確認原生質體介導的grf基因至少可以在受試動物體內滯留28天以上,為grf的長期表達提供了條件。Lovrin 10 and cv. 5389. by using this system, following questions were investigated : the change of the pattern of microtubules when treated with iwf ; the change of cytosolic calcium levels in the protoplasts when treated with iwf and the influence of microtubule depolymerization prior to iwf treatment on the levels of cytosolic calcium
試驗的目的是以激發子?原生質體簡化試驗系統來模擬葉銹菌侵染小麥葉片的互作體系,探討激發子刺激后寄主植物微管骨架的動態變化及胞內ca ~ ( 2 + )水平的變化規律,為進一步探討激發子誘導防衛反應的信號轉導途徑奠定基礎。Based on these observations, we isolated protoplasts from female germ unit and performed extracellular ca2 + influx experiment in order to check whether the cytological event similar to what happens during fertilization will be induced by extracellular ca2 + influx
表明細胞壁的合成是受精后早期發生的細胞學事件。在此基礎上,我們分離了胚囊細胞的原生質體。通過外源導入鈣離子檢查鈣能否引發類似受精過程發生的細胞學事件。Comparing with the old basic accounting standard, the new one has developed in many aspects : the property and status of the basic accounting standard are more explicit, the expression of objectives of financial reporting is more scientific, the expression of accounting principles is more refined and precise, the basic accounting standard generally constitute accounting elements and add the part of accounting measurement
摘要新的《企業會計準則基本準則》與原基本準則相比,主要在以下方面有了新發展:基本準則的性質與地位更加明確;財務會計報告目標的表述更加科學;會計核算一般原則的表述更加精練和準確;對會計要素從總體上加以規范;增加了會計計量部分。分享友人