質體缺失 的英文怎麼說

中文拼音 [zhítǐquēshī]
質體缺失 英文
apoplastidy
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 體 : 體構詞成分。
  • 缺 : Ⅰ動詞1 （缺乏; 短少） be short of; lack 2 （殘缺） be missing; be incomplete 3 （該到而未到） be ...
  • 缺失 : hiatus; deficiency; [遺傳學] deletion
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Most deletions are likely to be the result of two breaks with loss of the chromosomal material between them.

    大多數的缺失可能是由於發生了兩個斷裂，而兩個斷裂之間的那部分染色體物質失去了。
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. This article started with the background of " 3 + x " model, aimed to explore the conception and the essence of " 3 + x ", seeked for the gap between this model and actual biology teaching, disclosed the drawback of " education for tests " that existed in both idea and practice of teaching as well as the lack of role consciousness, efficiency and innovation, and consequently presented relevant teaching strategy and measures

    本課題從「 3 + x 」高考模式產生的背景中追根搠源，探討「 3 + x 」的命題立意及實質，從實際的中學生物學教學現狀的調查研究中找尋與之不相適應的差距，發現了教育教學中從觀念到實施所存在的「應試教育」的遺患，以及課堂教學中存在的主體性、有效性和創新性缺失等問題，進而提出了相應的教學策略和措施。
  5. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  6. Ie protein have multiple functions, such as conversion of the host cell into a metabolically activate states, activation of viral early genes in the early phase, repression of their own transcription. ie 180 proteins can stimulate transcription from both homologous such as the icp4 of hsv ( herpes simplex virus ) and heterologous viral and cellular promoter such as - globe protein

    經分析偽狂犬病病毒立即早期蛋白（ immediate - earlyprotein180 ， ie180 ）的蛋白質結構，發現ie180具有轉錄激活因子的結構特徵，能與類rna聚合酶結合， ie180基因缺失突變體對sv40及cmv啟動子調控作用的研究，是考察ie180能否作為轉錄激活因子應用的關鍵。
  7. However, in view of the legacy of system it has inherited, being eager to stabilize the political order, and the urgent need to resume and develop the economic construction, and the plan system carried out for a long time so that the higher education and national political and economical system formed a coupling body of same quality, resulting in noumenon deficiency, subject diasterna and position transferred passively

    然而，鑒於它所繼承的制度遺產以及急於穩定政治秩序、恢復和發展經濟建設的迫切需要，長期執行的計劃體制使高等教育與國家政治經濟體制構成同質耦合體，造成了高等教育的本體缺失、主體虛位和地位讓渡。尤其是面臨市場經濟體制和知識社會以及由此而導致的大眾化發展趨勢的挑戰，又出現了與其自身自主發展和健康發展不相適應的失范現象。
  8. Without it, nerves and muscles would cease to function, the absorption of major nutrients would be impaired, and the body would not be able to maintain adequate water and mineral balance

    鈉缺失時，神經和肌肉會喪失功能，主要營養成分的吸收會受損，機體也不能保持水和礦物質的適當平衡。
  9. Dental cobalt - chromium alloy casting as cobalt - chromium alloy, my company ' s current production of cobalt chromium alloy to form a rigid cylindrical, rectangular soft for two, mainly for dental patients with tooth loss or defect repair, organize and dense, strong corrosion resistance, biocompatibility, and good liquidity, easy casting, casting and polishing a hard flexible, white aesthetic characteristics of the dental practitioner ' s the ideal dental restoration materials

    牙科用鈷鉻鑄造合金簡稱鈷鉻合金，我公司目前生產的鈷鉻合金外形有硬質為圓柱形、軟質為長方形兩種，主要用於牙病患者牙齒的缺失或者缺損的修復，具有組織緻密、耐腐蝕性強、生物相容性好、流動性好、易於鑄造，鑄件拋光后具有質地堅硬有彈性、白亮美觀的特點，是牙科醫生獲得牙齒修復體的理想材料。
  10. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  11. A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss

    採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。
  12. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  13. This thesis advocates a fair and reasonable new order of ethic in the international culture field after analyzing the erroneous area in theory and the deviation in ethic of " the theory of civilization contradiction ", discussing the conflicts of value and the dialectical relationship between the globalization and localization of culture while points out the cultural nature and ethical shortcoming of the occidental centralism, examines the global ethic and the limit of probability for all people to share the cultural value, and reveals the significance of confucian culture as national symbol and value carrier in this age

    本文分析了「文明沖突論」的理論誤區和倫理偏頗，討論了文化全球化與文化本土化的價值沖突和辯證關系，指出了西方中心主義的文化本質和倫理缺失，探詢了全球倫理及其人類嘗試文化價值共享的可能性限度，揭示了中國儒家文化作為民族標識和價值載體的時代意義，主張在國際文化領域建立公正合理的倫理新秩序。
  14. The author thinks, the advantage of the quality of our entrepreneurial business ethics includes : pursue the confucian business ideal state, recognize entrepreneurial character and morals, pursue the entrepreneur spirit ; the disadvantage of the quality of our entrepreneurial business ethics incarnates : state entrepreneurial search for non - productive rent, private entrepreneurial non - standard management and the deficiency of credit

    筆者認為，我國企業家經濟倫理素質的優點主要是追求儒商境界、認同企業家品格和追求企業家精神；我國企業家經濟倫理素質的缺點主要體現為：國企企業家的非生產性尋租、私營企業家的錯位經營以及信用缺失。
  15. Investigation on agronomic characters and quality characters of these new soybean varieties

    大豆脂肪氧化酶缺失體的農藝和品質性狀鑒定
  16. The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )

    將gd 、 ge基因連接于質粒puc18獲得pugdge ，缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段，構建了通用轉移載體ppd m pe 。
  17. On improving teaching quality under the conditions of individualized learning

    對在集體缺失環境下提高教學質量的認識和實踐
  18. The quality education signs the spiritual concern lacking of the subject, object and content in the examination - oriented education system

    摘要素質教育時代的到來凸現了應試教育體系中精神關懷的主體、客體和內容的缺失。
  19. This part discusses the dilemma and functional loss of the existing tax law in the e - commerce circumstance through tax equity and legal principle, the rules of establishment of tax jurisdiction, ' the possibility of being traced of e - commerce trade course, the cognizant difficulty of the character of the taxed object in the circumstance and the uncertainty of tax law application

    本部分在第一部分的基礎，從稅收公平與法定原則、稅收管轄權確立規則、電子商務交易過程的可追溯性與電子商務環境下征稅對象性質的認定的困難及由此帶來的稅法適用上的不確定性出發，具體地論述了電子商務環境下現行稅法所面臨的困惑與功能缺失。
  20. The 16 ~ ( th ) national congress of the cpc proposed to further reform our judicial system and ensure the independent operation of procuratorate power, which ushered the reform of judicial system in our country into a new historic phase since then on. in order to realize the reasonable goal of reform of judicial system, we need carefully review our framework of state power from the point of view of law and correctly understand the nature and contents of legal system as well, since they are the base and safeguard of construction and function and implementation of state power, meanwhile the correct legal theory will be the prerequisite for realization of reasonable reform of judicial system. procuratorate power, one of the major basic powers included in our people ’ s congress system, together with the most supreme state power ( i. e. legislative power ) and administrative power and courts ’ judicial power and military power form the structure of a nation ’ s basic powers

    本文通過對檢察權的產生和發展歷史的綜合考察，分析比較了中西現代檢察權與古代檢察的變化與發展，梳理評析了中西已有的檢察權基本理論;從檢察權產生的理論基礎、權力的歸屬、行為特徵、權能組合的異質同構、權力的位階、檢察權功能的現代轉型等視角，剖析了現代檢察權的個性特徵;通過比較分析檢察權與法律監督權在性質、權力功能配置和實現方式等方面的差異，指出了中國檢察權定位為法律監督屬性的歷史局限性;結合現代檢察權的發展規律以及中國的國情，論證了在中國憲政語境中檢察權兼容法律監督權的合理性和正當性理由，提出了在相對合理主義下重新定位和解構中國檢察權的基本思路;深入分析了檢察權獨立行使的合理性和正當性;在比較分析中西檢察權獨立構成要素的基礎上，檢討了中國檢察權獨立的根本缺失在於精神要素、社會獨立主體和內部獨立操作規則的缺失，並提出了改革完善的基本思路。
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