赤醇 的英文怎麼說
中文拼音 [chìchún]
赤醇
英文
erythrol-
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。Ppsa and tkta are the key genes in central metabolism of aromatic amino acids biosynthesis. ppsa encoding phosphoenolpyrucate synthetase a ( ppsa ) which catalyzes pyruvate into pep ; tkta encoding transketolase a which plays a major role in erythrose - 4 - phosphate ( e4p ) production of pentose pathway
Ppsa基因編碼磷酸烯醇式丙酮酸合成酶a （ ppsa ） ，該酶催化丙酮酸合成磷酸烯醇式丙酮酸； tkta基因編碼轉酮酶a ，該酶在磷酸戊糖途徑中生成4 -磷酸赤蘚糖起主要作用。So it has increasing commercial demand. escherichia coli and many other microoganism synthesize aromatic amino acids through the condensation reaction between phosphoenolpyru vate ( pep ) and erythrose - 4 - phosphate ( e4p ) to form 3 - deoxy - d - arabinohep tulosonate 7 - phosphate ( dahp ). pep and e4p are limiting substrates for formation of dahp
大腸桿菌和許多微生物一樣，其合成芳香族氨基酸的起始物是由磷酸烯醇式丙酮酸（ pep ）和4 -磷酸赤癬糖（ e4p ）二者縮合形成的3 -脫氧- -阿拉伯庚酮糖酸- 7 -磷酸（ dahp ） 。Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。Triantafillopoulos n g ， dittman - mcbain c b ， westerman i j ， et al. polyvinyl alcohol copolymer composition ： us ， 6881778 [ p ]. 2005 ? 04 ? 19
夏赤丹，梅繼香.對三異氰酸三苯基硫代磷酸酯交聯聚乙烯醇乳膠[ j ] .中國膠粘劑， 2003 （ 5 ） ： 52Relaxing effects of phytoestrogen alpha - zearanol on the thoracic aorta in rats
玉米赤霉醇對大鼠胸主動脈血管的舒張效應The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +
主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals
首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。Imperial court cabernet sauvignon is distinguished by its deep ruby hue and full - bodied, well - balanced taste, with hints of black currant and berries
赤霞珠皇軒紅深沉動人的酒色，醇厚持久的酒香，都源於獨特的赤霞珠優質葡萄。Pichia pastoris gene expression system is a second generation expression system in yeast after the s. cerevisiae
甲醇畢赤酵母pichiapastoris表達系統是繼釀酒酵母之後的第二代酵母表達系統。Determination of zeranol in chicken rabbit liver by lc - esi - ms - ms
肝中玉米赤霉醇殘留量測定研究分享友人