載物片 的英文怎麼說

中文拼音 [zǎiwùpiān]
載物片 英文
micro slide
  • 載 : 載Ⅰ名詞（年） year : 一年半載 six to twelve months; six months to a year; 三年五載 three to five ...
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 片 : 片構詞成分。
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise

    本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。
  3. The chapter filmdom anecdotes written by george shen contains detailed information on yuen yang - an, a key figure of early great wall days. 416 page with over a hundred precious photos

    書中更收錄了沈鑒治撰寫的舊影話，篇中對早期長城的關鍵人物袁仰安有詳盡的介紹。全書416頁，中英對照，載有百多幀珍貴照片，並附各人的作品年表。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii

    並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。
  6. Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding pcr product from brussel sprouts, kohlrabi and kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon

    對各pcr產物分別進行回收、純化、載體連接和序列測定及基因結構分析等，結果表明，該片段在抱子甘藍、羽衣甘藍和球莖甘藍三種作物中的全長分別為1665bp 、 1650bp和1650bp ，包括相應基因的前兩個外顯子和內含子以及第三個外顯子的22bp序列。
  7. A comparison of the two movies as well as the characters nadia from nadia and sheeta from laputa

    -故事內容人物介紹圖片桌布區及提供音樂下載等。
  8. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  9. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  10. The leec biochip can be connected with pcb ( printed circuit board ), thus it can generate a moving electric field by changing time, scope and field intensity discretionarily under single chip processor ' s control. meanwhile it is probable to reduce driving voltage and decrease temperature greatly, and so increase resolution of dna separation

    研究內容包括線性分散式電極陣列的理論設計，以普通載波片和有機高聚物pdms （ polydimethylsiloxane ）為基本材料的晶元製作工藝， leec晶元和pcb板的連接方式，硬體控制系統的設計以及控制晶元工作的單片機程序編制等，此外還包括電化學檢測方法的研究。
  11. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  12. Information on u. c. gundam side story advance of zeta, galleries of gundam emblems, and notes on bandai s entertainment bible reference series

    -機動武鬥傳g高達g鋼彈的網站，有人物介紹，故事內容，圖片及mp3下載。
  13. Interview with all of the major voice talent for the english version of oh ! my goddess : scott simpson, juliet cesario, lanelle markgraf, and pamela weidner

    -介紹幸運女神的人物劇情電影音樂等基本資訊外，另含與女神有關的美術創作，並附有精品地圖和電影片預告下載。
  14. At the same time i also did many primary experments on separator and these polymer membrane can be used in as soft packaging li - ion battery ’ s separator. used polyvinylidene fluoride ( pvdf ) as the basic material, added cotton fibre and starch in, cooked with 100 in boiling water afer 3 hours, the starch inflate, and then acquire porous polymer membrane. in the process of coating polyester film, copper foil and aluminum boil were used to as carrier,

    選用的基體材料為pvdf ，同時向其中添加棉纖維和澱粉，最後利用100沸水對隔膜進行3小時蒸煮處理使澱粉溶脹，從而達到造孔的目的。在塗布工藝的篩選中，通過對麥臘片、銅箔、鋁箔進行篩選，最後選定以銅箔為載體來進行塗布處理。當以銅箔為載體時，可以使隔膜正反兩面物理性質達到基本一致。
  15. Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed

    構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。
  16. The full length cdnas of 17 - hsd1 and 17 - hsd3, 17 - hsd8 were obtained by library screening and race, respectively. expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot. the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3. 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell

    首先從genebank下載在其他脊椎動物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，並在序列保守區域設計簡並引物，然後分別以羅非魚卵巢和精巢cdna為模板進行rt - pcr擴增得到17 - hsd1 ， 17 - hsd3的中間片段。
  17. Muscle is comprised of thick and thin myofilaments that slide across each other during contraction

    肌肉是由厚與薄的肌原絲所組成，這些肌原絲透過其他在收縮之間的載物片。
  18. A five - phase numerical model based on prism coupled is derived from maxwell equation and fresnel equation. using the professional software - mathcad, characteristic of this numerical model is simulated. the relationships between depths of the metal film, bio - sensitive film, cover slide, the refractive index of bio - sensitive film and the resonant angle ospr has been discussed respectively

    根據maxwell方程和fresnel方程推導出5層棱鏡耦合spr數值化模型，利用數學軟體mathcad對該數學模型進行了模擬，討論了金屬膜厚度d _ 2 、生物膜厚度d _ 3和折射率n _ 3 、載玻片厚度d _ 1與諧振峰值_ （ spr ）的關系。
  19. This paper mainly introduces technologies of bending carbon fiber reinforced plastic ( cfrp ) plates or fabrics to the web of reinforced concrete members, and also this paper discusses the design principles of carbon fiber reinforced plastic ( cfrp ) used in the reinforced concrete members, as well as considering the preload effect to the bending capacity of the reinforced concrete members. finally, a kind of new practical method is put forward, and may be a reference for practical engineering designer. in the meantime, this paper analyzes the " local failure " caused by shear and normal stress concentrations at the plate ends that can easily incorporated into design equations. furthermore, the bonding and anchoring conformation of concrete members strengthened by carbon fiber reinforced plastics also be set forth

    本文重點研究碳纖維製成織物片材粘貼到混凝土表面用於結構的補強與加固的技術特點，從理論上探討cfrp加固混凝土結構的設計原理，分析二次受力對加固結構的彎矩承載力的影響；同時對外貼碳纖維加固混凝土的抗彎和抗剪提供一種簡單、實用的設計方法，供工程設計人員參考，對碳纖維布加固混凝土梁中出現剝離破壞也進行了分析，提供了最大剝離正應力的計算公式，以及對外貼cfrp加固結構構件的粘貼構造與錨固進行了闡述。
  20. It is proved by the simulation that the temperature of samples can be estimated from the thermo - couple placed on the cover glass and the planar temperature distribution of the cryostage is approximately uniform

    分析結果表明：可以利用布置在蓋玻片上表面的熱電偶測量並推算實驗樣品層的溫度；低溫臺載物表面溫度均勻。
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